Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04450 and AMB_RS04455 are separated by 10 nucleotides AMB_RS04455 and AMB_RS04460 are separated by 76 nucleotides AMB_RS04460 and AMB_RS04465 are separated by 112 nucleotides
AMB_RS04450: AMB_RS04450 - ankyrin repeat domain-containing protein, at 932,664 to 933,629
_RS04450
AMB_RS04455: AMB_RS04455 - energy-dependent translational throttle protein EttA, at 933,640 to 935,319
_RS04455
AMB_RS04460: AMB_RS04460 - hypothetical protein, at 935,396 to 935,791
_RS04460
AMB_RS04465: AMB_RS04465 - ribonuclease R, at 935,904 to 938,132
_RS04465
Position (kb) 933
934
935
936 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 932.668 kb on + strand at 932.668 kb on + strand at 932.669 kb on - strand at 932.669 kb on - strand at 932.670 kb on + strand at 932.670 kb on + strand at 932.670 kb on + strand at 932.670 kb on + strand at 932.670 kb on + strand at 932.671 kb on - strand at 932.671 kb on - strand at 932.671 kb on - strand at 932.671 kb on - strand at 932.728 kb on + strand at 932.729 kb on - strand at 932.729 kb on - strand at 933.112 kb on + strand, within AMB_RS04450 at 933.113 kb on - strand, within AMB_RS04450 at 933.113 kb on - strand, within AMB_RS04450 at 933.113 kb on - strand, within AMB_RS04450 at 933.193 kb on + strand, within AMB_RS04450 at 933.193 kb on + strand, within AMB_RS04450 at 933.194 kb on - strand, within AMB_RS04450 at 933.194 kb on - strand, within AMB_RS04450 at 933.194 kb on - strand, within AMB_RS04450 at 933.194 kb on - strand, within AMB_RS04450 at 933.194 kb on - strand, within AMB_RS04450 at 933.194 kb on - strand, within AMB_RS04450 at 933.194 kb on - strand, within AMB_RS04450 at 933.194 kb on - strand, within AMB_RS04450 at 933.248 kb on - strand, within AMB_RS04450 at 933.618 kb on - strand at 933.659 kb on + strand at 933.660 kb on - strand at 933.660 kb on - strand at 933.660 kb on - strand at 933.660 kb on - strand at 933.719 kb on + strand at 933.719 kb on + strand at 933.719 kb on + strand at 933.720 kb on - strand at 933.720 kb on - strand at 933.720 kb on - strand at 933.720 kb on - strand at 933.720 kb on - strand at 933.720 kb on - strand at 933.728 kb on + strand at 933.728 kb on + strand at 933.728 kb on + strand at 933.729 kb on - strand at 933.729 kb on - strand at 934.028 kb on + strand, within AMB_RS04455 at 934.140 kb on - strand, within AMB_RS04455 at 934.140 kb on - strand, within AMB_RS04455 at 934.140 kb on - strand, within AMB_RS04455 at 934.140 kb on - strand, within AMB_RS04455 at 934.140 kb on - strand, within AMB_RS04455 at 934.140 kb on - strand, within AMB_RS04455 at 934.292 kb on + strand, within AMB_RS04455 at 934.292 kb on + strand, within AMB_RS04455 at 934.292 kb on + strand, within AMB_RS04455 at 934.292 kb on + strand, within AMB_RS04455 at 934.292 kb on + strand, within AMB_RS04455 at 934.293 kb on - strand, within AMB_RS04455 at 934.293 kb on - strand, within AMB_RS04455 at 934.293 kb on - strand, within AMB_RS04455 at 934.438 kb on - strand, within AMB_RS04455 at 934.438 kb on - strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.577 kb on + strand, within AMB_RS04455 at 934.578 kb on - strand, within AMB_RS04455 at 934.578 kb on - strand, within AMB_RS04455 at 934.589 kb on + strand, within AMB_RS04455 at 934.590 kb on - strand, within AMB_RS04455 at 934.590 kb on - strand, within AMB_RS04455 at 934.649 kb on + strand, within AMB_RS04455 at 934.650 kb on - strand, within AMB_RS04455 at 934.650 kb on - strand, within AMB_RS04455 at 934.650 kb on - strand, within AMB_RS04455 at 934.685 kb on + strand, within AMB_RS04455 at 934.686 kb on - strand, within AMB_RS04455 at 934.686 kb on - strand, within AMB_RS04455 at 934.686 kb on - strand, within AMB_RS04455 at 934.686 kb on - strand, within AMB_RS04455 at 934.686 kb on - strand, within AMB_RS04455 at 935.096 kb on + strand, within AMB_RS04455 at 935.096 kb on + strand, within AMB_RS04455 at 935.097 kb on - strand, within AMB_RS04455 at 935.097 kb on - strand, within AMB_RS04455 at 935.138 kb on + strand, within AMB_RS04455 at 935.139 kb on - strand, within AMB_RS04455 at 935.270 kb on + strand at 935.297 kb on + strand at 935.297 kb on + strand at 935.297 kb on + strand at 935.297 kb on + strand at 935.297 kb on + strand at 935.297 kb on + strand at 935.297 kb on + strand at 935.297 kb on + strand at 935.297 kb on + strand at 935.297 kb on + strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.298 kb on - strand at 935.303 kb on + strand at 935.303 kb on + strand at 935.303 kb on + strand at 935.303 kb on + strand at 935.303 kb on + strand at 935.303 kb on + strand at 935.303 kb on + strand at 935.303 kb on + strand at 935.304 kb on - strand at 935.304 kb on - strand at 935.304 kb on - strand at 935.304 kb on - strand at 935.304 kb on - strand at 935.309 kb on + strand at 935.309 kb on + strand at 935.309 kb on + strand at 935.310 kb on - strand at 935.310 kb on - strand at 935.310 kb on - strand at 935.310 kb on - strand at 935.361 kb on + strand at 935.582 kb on + strand, within AMB_RS04460 at 935.711 kb on + strand, within AMB_RS04460 at 935.774 kb on + strand at 935.774 kb on + strand at 935.775 kb on - strand at 935.775 kb on - strand at 935.890 kb on + strand at 935.891 kb on - strand at 935.891 kb on - strand at 935.892 kb on + strand at 935.893 kb on - strand at 936.190 kb on - strand, within AMB_RS04465 at 936.190 kb on - strand, within AMB_RS04465 at 936.190 kb on - strand, within AMB_RS04465 at 936.309 kb on + strand, within AMB_RS04465 at 936.309 kb on + strand, within AMB_RS04465 at 936.309 kb on + strand, within AMB_RS04465 at 936.309 kb on + strand, within AMB_RS04465 at 936.310 kb on - strand, within AMB_RS04465 at 936.310 kb on - strand, within AMB_RS04465
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 932,668 + -0.6 932,668 + -0.2 932,669 - -0.1 932,669 - -0.1 932,670 + -0.5 932,670 + +0.2 932,670 + -1.8 932,670 + -0.7 932,670 + -0.3 932,671 - -0.2 932,671 - -0.0 932,671 - -0.6 932,671 - -0.9 932,728 + +0.3 932,729 - +0.4 932,729 - +0.6 933,112 + AMB_RS04450 0.46 -0.5 933,113 - AMB_RS04450 0.46 -1.8 933,113 - AMB_RS04450 0.46 +0.4 933,113 - AMB_RS04450 0.46 -0.3 933,193 + AMB_RS04450 0.55 +0.1 933,193 + AMB_RS04450 0.55 +0.2 933,194 - AMB_RS04450 0.55 -0.6 933,194 - AMB_RS04450 0.55 -0.0 933,194 - AMB_RS04450 0.55 +0.3 933,194 - AMB_RS04450 0.55 -0.1 933,194 - AMB_RS04450 0.55 -1.0 933,194 - AMB_RS04450 0.55 +0.3 933,194 - AMB_RS04450 0.55 +0.3 933,194 - AMB_RS04450 0.55 +0.1 933,248 - AMB_RS04450 0.60 -0.0 933,618 - +0.2 933,659 + +0.1 933,660 - -0.7 933,660 - +2.0 933,660 - +0.6 933,660 - -0.2 933,719 + -0.0 933,719 + +0.6 933,719 + +1.1 933,720 - -1.0 933,720 - -0.3 933,720 - -0.3 933,720 - -0.6 933,720 - -1.9 933,720 - -0.2 933,728 + +0.1 933,728 + -0.0 933,728 + +0.3 933,729 - -0.9 933,729 - -1.6 934,028 + AMB_RS04455 0.23 -0.9 934,140 - AMB_RS04455 0.30 -0.1 934,140 - AMB_RS04455 0.30 -1.4 934,140 - AMB_RS04455 0.30 -0.0 934,140 - AMB_RS04455 0.30 -0.5 934,140 - AMB_RS04455 0.30 -1.5 934,140 - AMB_RS04455 0.30 +0.1 934,292 + AMB_RS04455 0.39 +0.1 934,292 + AMB_RS04455 0.39 -0.3 934,292 + AMB_RS04455 0.39 -3.2 934,292 + AMB_RS04455 0.39 +1.0 934,292 + AMB_RS04455 0.39 -2.0 934,293 - AMB_RS04455 0.39 +0.2 934,293 - AMB_RS04455 0.39 -0.8 934,293 - AMB_RS04455 0.39 -0.7 934,438 - AMB_RS04455 0.47 +0.2 934,438 - AMB_RS04455 0.47 -0.0 934,577 + AMB_RS04455 0.56 -0.4 934,577 + AMB_RS04455 0.56 -1.3 934,577 + AMB_RS04455 0.56 -0.9 934,577 + AMB_RS04455 0.56 -0.0 934,577 + AMB_RS04455 0.56 -0.8 934,577 + AMB_RS04455 0.56 -1.0 934,577 + AMB_RS04455 0.56 -0.8 934,577 + AMB_RS04455 0.56 +0.1 934,577 + AMB_RS04455 0.56 -0.2 934,577 + AMB_RS04455 0.56 -0.4 934,578 - AMB_RS04455 0.56 +1.2 934,578 - AMB_RS04455 0.56 -0.7 934,589 + AMB_RS04455 0.56 -0.6 934,590 - AMB_RS04455 0.57 +0.3 934,590 - AMB_RS04455 0.57 -1.4 934,649 + AMB_RS04455 0.60 -0.5 934,650 - AMB_RS04455 0.60 -0.5 934,650 - AMB_RS04455 0.60 -1.0 934,650 - AMB_RS04455 0.60 -1.6 934,685 + AMB_RS04455 0.62 -0.5 934,686 - AMB_RS04455 0.62 -1.7 934,686 - AMB_RS04455 0.62 -0.3 934,686 - AMB_RS04455 0.62 -1.7 934,686 - AMB_RS04455 0.62 +0.1 934,686 - AMB_RS04455 0.62 -0.8 935,096 + AMB_RS04455 0.87 +0.1 935,096 + AMB_RS04455 0.87 -0.0 935,097 - AMB_RS04455 0.87 -1.0 935,097 - AMB_RS04455 0.87 +0.0 935,138 + AMB_RS04455 0.89 -0.6 935,139 - AMB_RS04455 0.89 -0.4 935,270 + -0.8 935,297 + -2.4 935,297 + -0.9 935,297 + -1.9 935,297 + -2.2 935,297 + -0.8 935,297 + -3.2 935,297 + +0.5 935,297 + -3.5 935,297 + -0.3 935,297 + +0.0 935,298 - -1.0 935,298 - -0.2 935,298 - +0.4 935,298 - -0.9 935,298 - -1.0 935,298 - -0.4 935,298 - +0.3 935,298 - -2.2 935,298 - +0.0 935,298 - -0.4 935,298 - -0.1 935,298 - +1.7 935,298 - -0.1 935,303 + -0.2 935,303 + -0.7 935,303 + -0.7 935,303 + -0.7 935,303 + +0.6 935,303 + -1.4 935,303 + +0.5 935,303 + -1.3 935,304 - +0.3 935,304 - -0.5 935,304 - -0.3 935,304 - -1.4 935,304 - -0.5 935,309 + -0.9 935,309 + -0.4 935,309 + -2.9 935,310 - -0.1 935,310 - -0.0 935,310 - -0.1 935,310 - -0.3 935,361 + +0.1 935,582 + AMB_RS04460 0.47 -2.2 935,711 + AMB_RS04460 0.80 -0.6 935,774 + +0.1 935,774 + -0.5 935,775 - +0.2 935,775 - -0.2 935,890 + -3.9 935,891 - -0.2 935,891 - -0.2 935,892 + -0.5 935,893 - +1.5 936,190 - AMB_RS04465 0.13 -0.4 936,190 - AMB_RS04465 0.13 +0.1 936,190 - AMB_RS04465 0.13 -0.4 936,309 + AMB_RS04465 0.18 -0.6 936,309 + AMB_RS04465 0.18 +0.4 936,309 + AMB_RS04465 0.18 -0.9 936,309 + AMB_RS04465 0.18 +0.0 936,310 - AMB_RS04465 0.18 +0.0 936,310 - AMB_RS04465 0.18 -0.5
Or see this region's nucleotide sequence