Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04315 and AMB_RS04320 are separated by 62 nucleotides AMB_RS04320 and AMB_RS04325 are separated by 3 nucleotides AMB_RS04325 and AMB_RS04330 overlap by 11 nucleotides
AMB_RS04315: AMB_RS04315 - PAS domain S-box protein, at 907,203 to 909,743
_RS04315
AMB_RS04320: AMB_RS04320 - DNA-binding response regulator, at 909,806 to 910,528
_RS04320
AMB_RS04325: AMB_RS04325 - TetR/AcrR family transcriptional regulator, at 910,532 to 911,140
_RS04325
AMB_RS04330: AMB_RS04330 - xanthine and CO dehydrogenase maturation factor, at 911,130 to 911,909
_RS04330
Position (kb) 909
910
911 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 908.920 kb on - strand, within AMB_RS04315 at 908.920 kb on - strand, within AMB_RS04315 at 909.135 kb on + strand, within AMB_RS04315 at 909.136 kb on - strand, within AMB_RS04315 at 909.136 kb on - strand, within AMB_RS04315 at 909.170 kb on - strand, within AMB_RS04315 at 909.170 kb on - strand, within AMB_RS04315 at 909.559 kb on - strand at 909.606 kb on + strand at 909.606 kb on + strand at 909.607 kb on - strand at 909.607 kb on - strand at 909.607 kb on - strand at 909.607 kb on - strand at 909.607 kb on - strand at 909.679 kb on + strand at 909.680 kb on - strand at 909.688 kb on + strand at 909.689 kb on - strand at 909.762 kb on - strand at 909.817 kb on - strand at 909.854 kb on - strand at 909.854 kb on - strand at 909.863 kb on + strand at 909.863 kb on + strand at 910.097 kb on + strand, within AMB_RS04320 at 910.097 kb on + strand, within AMB_RS04320 at 910.097 kb on + strand, within AMB_RS04320 at 910.098 kb on - strand, within AMB_RS04320 at 910.379 kb on + strand, within AMB_RS04320 at 910.390 kb on + strand, within AMB_RS04320 at 910.391 kb on - strand, within AMB_RS04320 at 910.391 kb on - strand, within AMB_RS04320 at 910.391 kb on - strand, within AMB_RS04320 at 910.481 kb on + strand at 910.482 kb on - strand at 910.482 kb on - strand at 910.482 kb on - strand at 910.527 kb on - strand at 910.604 kb on + strand, within AMB_RS04325 at 910.605 kb on - strand, within AMB_RS04325 at 910.605 kb on - strand, within AMB_RS04325 at 910.676 kb on + strand, within AMB_RS04325 at 910.676 kb on + strand, within AMB_RS04325 at 910.676 kb on + strand, within AMB_RS04325 at 910.677 kb on - strand, within AMB_RS04325 at 910.677 kb on - strand, within AMB_RS04325 at 910.709 kb on + strand, within AMB_RS04325 at 910.710 kb on - strand, within AMB_RS04325 at 910.729 kb on + strand, within AMB_RS04325 at 910.729 kb on + strand, within AMB_RS04325 at 910.829 kb on + strand, within AMB_RS04325 at 910.829 kb on + strand, within AMB_RS04325 at 910.830 kb on - strand, within AMB_RS04325 at 910.830 kb on - strand, within AMB_RS04325 at 910.899 kb on - strand, within AMB_RS04325 at 910.899 kb on - strand, within AMB_RS04325 at 910.929 kb on - strand, within AMB_RS04325 at 911.009 kb on + strand, within AMB_RS04325 at 911.009 kb on + strand, within AMB_RS04325 at 911.010 kb on - strand, within AMB_RS04325 at 911.093 kb on + strand at 911.093 kb on + strand at 911.094 kb on - strand at 911.094 kb on - strand at 911.094 kb on - strand at 911.274 kb on + strand, within AMB_RS04330 at 911.274 kb on + strand, within AMB_RS04330 at 911.274 kb on + strand, within AMB_RS04330 at 911.275 kb on - strand, within AMB_RS04330 at 911.275 kb on - strand, within AMB_RS04330
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 908,920 - AMB_RS04315 0.68 +0.6 908,920 - AMB_RS04315 0.68 -1.0 909,135 + AMB_RS04315 0.76 -1.6 909,136 - AMB_RS04315 0.76 -0.1 909,136 - AMB_RS04315 0.76 -0.2 909,170 - AMB_RS04315 0.77 -0.1 909,170 - AMB_RS04315 0.77 -0.2 909,559 - -0.4 909,606 + +0.1 909,606 + -0.9 909,607 - -0.3 909,607 - -1.0 909,607 - +0.2 909,607 - +2.5 909,607 - -0.8 909,679 + -0.4 909,680 - -0.2 909,688 + -0.2 909,689 - -0.5 909,762 - -0.5 909,817 - +0.2 909,854 - -1.9 909,854 - +0.1 909,863 + -0.1 909,863 + +0.2 910,097 + AMB_RS04320 0.40 -0.4 910,097 + AMB_RS04320 0.40 -0.2 910,097 + AMB_RS04320 0.40 -2.4 910,098 - AMB_RS04320 0.40 -0.0 910,379 + AMB_RS04320 0.79 +0.5 910,390 + AMB_RS04320 0.81 -1.1 910,391 - AMB_RS04320 0.81 +1.1 910,391 - AMB_RS04320 0.81 +0.7 910,391 - AMB_RS04320 0.81 -2.6 910,481 + -0.3 910,482 - -0.9 910,482 - -2.2 910,482 - -0.9 910,527 - -0.7 910,604 + AMB_RS04325 0.12 +1.0 910,605 - AMB_RS04325 0.12 -0.3 910,605 - AMB_RS04325 0.12 -0.3 910,676 + AMB_RS04325 0.24 +0.7 910,676 + AMB_RS04325 0.24 -0.0 910,676 + AMB_RS04325 0.24 -0.3 910,677 - AMB_RS04325 0.24 -1.8 910,677 - AMB_RS04325 0.24 -1.8 910,709 + AMB_RS04325 0.29 -0.4 910,710 - AMB_RS04325 0.29 -1.0 910,729 + AMB_RS04325 0.32 -0.0 910,729 + AMB_RS04325 0.32 -0.5 910,829 + AMB_RS04325 0.49 -2.0 910,829 + AMB_RS04325 0.49 -0.8 910,830 - AMB_RS04325 0.49 -0.0 910,830 - AMB_RS04325 0.49 +0.3 910,899 - AMB_RS04325 0.60 -2.0 910,899 - AMB_RS04325 0.60 +0.3 910,929 - AMB_RS04325 0.65 +0.6 911,009 + AMB_RS04325 0.78 -2.9 911,009 + AMB_RS04325 0.78 -1.2 911,010 - AMB_RS04325 0.78 +0.5 911,093 + -0.2 911,093 + -0.9 911,094 - -0.0 911,094 - -0.5 911,094 - -0.3 911,274 + AMB_RS04330 0.18 -0.4 911,274 + AMB_RS04330 0.18 -0.2 911,274 + AMB_RS04330 0.18 +1.0 911,275 - AMB_RS04330 0.19 +0.1 911,275 - AMB_RS04330 0.19 -0.7
Or see this region's nucleotide sequence