Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03875 and AMB_RS03880 overlap by 6 nucleotides AMB_RS03880 and AMB_RS03885 are separated by 107 nucleotides AMB_RS03885 and AMB_RS03890 are separated by 13 nucleotides
AMB_RS03875: AMB_RS03875 - NUDIX domain-containing protein, at 814,370 to 814,876
_RS03875
AMB_RS03880: AMB_RS03880 - tetratricopeptide repeat-containing protein, at 814,871 to 815,638
_RS03880
AMB_RS03885: AMB_RS03885 - pentapeptide repeat-containing protein, at 815,746 to 816,240
_RS03885
AMB_RS03890: AMB_RS03890 - GGDEF domain-containing protein, at 816,254 to 817,312
_RS03890
Position (kb) 815
816
817 Strain fitness (log2 ratio) -2
-1
0
1
2 at 814.799 kb on + strand, within AMB_RS03875 at 814.800 kb on - strand, within AMB_RS03875 at 814.800 kb on - strand, within AMB_RS03875 at 814.826 kb on + strand at 814.826 kb on + strand at 814.826 kb on + strand at 814.826 kb on + strand at 814.873 kb on - strand at 814.873 kb on - strand at 814.873 kb on - strand at 814.874 kb on + strand at 814.874 kb on + strand at 814.875 kb on - strand at 814.875 kb on - strand at 815.293 kb on - strand, within AMB_RS03880 at 815.293 kb on - strand, within AMB_RS03880 at 815.293 kb on - strand, within AMB_RS03880 at 815.293 kb on - strand, within AMB_RS03880 at 815.293 kb on - strand, within AMB_RS03880 at 815.313 kb on + strand, within AMB_RS03880 at 815.313 kb on + strand, within AMB_RS03880 at 815.314 kb on - strand, within AMB_RS03880 at 815.314 kb on - strand, within AMB_RS03880 at 815.314 kb on - strand, within AMB_RS03880 at 815.434 kb on - strand, within AMB_RS03880 at 815.434 kb on - strand, within AMB_RS03880 at 815.434 kb on - strand, within AMB_RS03880 at 815.679 kb on + strand at 815.699 kb on - strand at 815.705 kb on + strand at 815.705 kb on + strand at 815.705 kb on + strand at 815.714 kb on + strand at 815.715 kb on - strand at 815.734 kb on + strand at 815.745 kb on + strand at 815.746 kb on - strand at 815.746 kb on - strand at 815.854 kb on + strand, within AMB_RS03885 at 815.855 kb on - strand, within AMB_RS03885 at 815.855 kb on - strand, within AMB_RS03885 at 815.855 kb on - strand, within AMB_RS03885 at 815.979 kb on + strand, within AMB_RS03885 at 815.979 kb on + strand, within AMB_RS03885 at 816.100 kb on + strand, within AMB_RS03885 at 816.100 kb on + strand, within AMB_RS03885 at 816.249 kb on + strand at 816.250 kb on - strand at 816.350 kb on + strand at 816.350 kb on + strand at 816.351 kb on - strand at 816.351 kb on - strand at 816.351 kb on - strand at 816.351 kb on - strand at 816.351 kb on - strand at 816.368 kb on + strand, within AMB_RS03890 at 816.368 kb on + strand, within AMB_RS03890 at 816.368 kb on + strand, within AMB_RS03890 at 816.368 kb on + strand, within AMB_RS03890 at 816.369 kb on - strand, within AMB_RS03890 at 816.369 kb on - strand, within AMB_RS03890 at 816.369 kb on - strand, within AMB_RS03890 at 816.369 kb on - strand, within AMB_RS03890 at 816.461 kb on + strand, within AMB_RS03890 at 816.462 kb on - strand, within AMB_RS03890 at 816.552 kb on - strand, within AMB_RS03890 at 816.552 kb on - strand, within AMB_RS03890 at 816.552 kb on - strand, within AMB_RS03890 at 816.584 kb on + strand, within AMB_RS03890 at 816.585 kb on - strand, within AMB_RS03890 at 816.929 kb on + strand, within AMB_RS03890 at 816.929 kb on + strand, within AMB_RS03890 at 816.930 kb on - strand, within AMB_RS03890 at 816.930 kb on - strand, within AMB_RS03890 at 816.930 kb on - strand, within AMB_RS03890 at 817.232 kb on + strand at 817.232 kb on + strand at 817.232 kb on + strand at 817.232 kb on + strand at 817.232 kb on + strand at 817.232 kb on + strand at 817.232 kb on + strand at 817.233 kb on - strand at 817.233 kb on - strand at 817.233 kb on - strand at 817.233 kb on - strand at 817.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 814,799 + AMB_RS03875 0.85 -1.1 814,800 - AMB_RS03875 0.85 +0.4 814,800 - AMB_RS03875 0.85 -0.2 814,826 + -0.9 814,826 + -0.3 814,826 + -0.5 814,826 + +0.0 814,873 - +0.0 814,873 - -0.4 814,873 - -0.2 814,874 + -0.2 814,874 + -1.2 814,875 - +0.7 814,875 - +0.3 815,293 - AMB_RS03880 0.55 -0.3 815,293 - AMB_RS03880 0.55 +0.2 815,293 - AMB_RS03880 0.55 +0.2 815,293 - AMB_RS03880 0.55 -0.4 815,293 - AMB_RS03880 0.55 +0.3 815,313 + AMB_RS03880 0.58 -0.2 815,313 + AMB_RS03880 0.58 +0.3 815,314 - AMB_RS03880 0.58 -0.4 815,314 - AMB_RS03880 0.58 -1.0 815,314 - AMB_RS03880 0.58 -0.3 815,434 - AMB_RS03880 0.73 +0.3 815,434 - AMB_RS03880 0.73 -0.2 815,434 - AMB_RS03880 0.73 +0.1 815,679 + +0.2 815,699 - -0.1 815,705 + +0.1 815,705 + -1.2 815,705 + -0.5 815,714 + +0.4 815,715 - -1.6 815,734 + -0.3 815,745 + -1.1 815,746 - -0.4 815,746 - +0.4 815,854 + AMB_RS03885 0.22 -0.1 815,855 - AMB_RS03885 0.22 -1.2 815,855 - AMB_RS03885 0.22 -0.1 815,855 - AMB_RS03885 0.22 +0.3 815,979 + AMB_RS03885 0.47 -2.6 815,979 + AMB_RS03885 0.47 +0.4 816,100 + AMB_RS03885 0.72 -0.1 816,100 + AMB_RS03885 0.72 -0.2 816,249 + +0.2 816,250 - -0.0 816,350 + +0.8 816,350 + -1.6 816,351 - -2.6 816,351 - +0.4 816,351 - -1.5 816,351 - -1.0 816,351 - -0.5 816,368 + AMB_RS03890 0.11 -0.1 816,368 + AMB_RS03890 0.11 -0.3 816,368 + AMB_RS03890 0.11 +0.7 816,368 + AMB_RS03890 0.11 -0.5 816,369 - AMB_RS03890 0.11 -0.0 816,369 - AMB_RS03890 0.11 +0.5 816,369 - AMB_RS03890 0.11 -0.1 816,369 - AMB_RS03890 0.11 +0.1 816,461 + AMB_RS03890 0.20 -0.6 816,462 - AMB_RS03890 0.20 +0.2 816,552 - AMB_RS03890 0.28 -0.6 816,552 - AMB_RS03890 0.28 -0.2 816,552 - AMB_RS03890 0.28 -0.1 816,584 + AMB_RS03890 0.31 -0.5 816,585 - AMB_RS03890 0.31 -0.3 816,929 + AMB_RS03890 0.64 -0.5 816,929 + AMB_RS03890 0.64 +0.1 816,930 - AMB_RS03890 0.64 -0.5 816,930 - AMB_RS03890 0.64 -0.1 816,930 - AMB_RS03890 0.64 -1.1 817,232 + -0.6 817,232 + -0.5 817,232 + +0.1 817,232 + +2.5 817,232 + -0.1 817,232 + -0.6 817,232 + -0.3 817,233 - -0.4 817,233 - +0.1 817,233 - -0.2 817,233 - +0.4 817,233 - +0.2
Or see this region's nucleotide sequence