Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03255 and AMB_RS03260 are separated by 15 nucleotides AMB_RS03260 and AMB_RS03265 are separated by 24 nucleotides AMB_RS03265 and AMB_RS03270 are separated by 115 nucleotides AMB_RS03270 and AMB_RS03275 are separated by 503 nucleotides
AMB_RS03255: AMB_RS03255 - bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase, at 680,941 to 681,780
_RS03255
AMB_RS03260: AMB_RS03260 - hypothetical protein, at 681,796 to 682,155
_RS03260
AMB_RS03265: AMB_RS03265 - enoyl-CoA hydratase, at 682,180 to 682,956
_RS03265
AMB_RS03270: AMB_RS03270 - 30S ribosomal protein S20, at 683,072 to 683,344
_RS03270
AMB_RS03275: AMB_RS03275 - chromosomal replication initiator protein DnaA, at 683,848 to 685,248
_RS03275
Position (kb)
682
683 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.641 kb on - strand, within AMB_RS03255 at 681.664 kb on + strand, within AMB_RS03255 at 681.665 kb on - strand, within AMB_RS03255 at 681.665 kb on - strand, within AMB_RS03255 at 681.665 kb on - strand, within AMB_RS03255 at 681.757 kb on + strand at 681.758 kb on - strand at 681.758 kb on - strand at 681.758 kb on - strand at 681.758 kb on - strand at 681.784 kb on + strand at 681.784 kb on + strand at 681.784 kb on + strand at 681.784 kb on + strand at 681.784 kb on + strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.786 kb on + strand at 681.786 kb on + strand at 681.786 kb on + strand at 681.786 kb on + strand at 681.787 kb on - strand at 681.787 kb on - strand at 681.787 kb on - strand at 681.787 kb on - strand at 681.787 kb on - strand at 681.787 kb on - strand at 681.886 kb on + strand, within AMB_RS03260 at 681.886 kb on + strand, within AMB_RS03260 at 681.977 kb on - strand, within AMB_RS03260 at 681.977 kb on - strand, within AMB_RS03260 at 681.977 kb on - strand, within AMB_RS03260 at 681.977 kb on - strand, within AMB_RS03260 at 681.977 kb on - strand, within AMB_RS03260 at 681.977 kb on - strand, within AMB_RS03260 at 681.977 kb on - strand, within AMB_RS03260 at 681.977 kb on - strand, within AMB_RS03260 at 682.005 kb on + strand, within AMB_RS03260 at 682.018 kb on + strand, within AMB_RS03260 at 682.070 kb on - strand, within AMB_RS03260 at 682.161 kb on + strand at 682.162 kb on - strand at 682.402 kb on + strand, within AMB_RS03265 at 682.402 kb on + strand, within AMB_RS03265 at 682.402 kb on + strand, within AMB_RS03265 at 682.402 kb on + strand, within AMB_RS03265 at 682.402 kb on + strand, within AMB_RS03265 at 682.402 kb on + strand, within AMB_RS03265 at 682.403 kb on - strand, within AMB_RS03265 at 682.403 kb on - strand, within AMB_RS03265 at 682.403 kb on - strand, within AMB_RS03265 at 682.403 kb on - strand, within AMB_RS03265 at 682.403 kb on - strand, within AMB_RS03265 at 682.403 kb on - strand, within AMB_RS03265 at 682.403 kb on - strand, within AMB_RS03265 at 682.403 kb on - strand, within AMB_RS03265 at 682.414 kb on + strand, within AMB_RS03265 at 682.414 kb on + strand, within AMB_RS03265 at 682.414 kb on + strand, within AMB_RS03265 at 682.414 kb on + strand, within AMB_RS03265 at 682.414 kb on + strand, within AMB_RS03265 at 682.414 kb on + strand, within AMB_RS03265 at 682.415 kb on - strand, within AMB_RS03265 at 682.415 kb on - strand, within AMB_RS03265 at 682.415 kb on - strand, within AMB_RS03265 at 682.415 kb on - strand, within AMB_RS03265 at 682.415 kb on - strand, within AMB_RS03265 at 683.025 kb on + strand at 683.342 kb on + strand at 683.356 kb on + strand at 683.356 kb on + strand at 683.356 kb on + strand at 683.357 kb on - strand at 683.359 kb on + strand at 683.676 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 681,466 + AMB_RS03255 0.62 +0.0 681,466 + AMB_RS03255 0.62 -0.1 681,466 + AMB_RS03255 0.62 -0.6 681,466 + AMB_RS03255 0.62 -0.1 681,466 + AMB_RS03255 0.62 -0.7 681,466 + AMB_RS03255 0.62 +0.1 681,466 + AMB_RS03255 0.62 -0.4 681,467 - AMB_RS03255 0.63 +0.5 681,467 - AMB_RS03255 0.63 -0.2 681,467 - AMB_RS03255 0.63 +0.9 681,467 - AMB_RS03255 0.63 +0.3 681,467 - AMB_RS03255 0.63 -0.6 681,467 - AMB_RS03255 0.63 -0.3 681,467 - AMB_RS03255 0.63 +0.1 681,467 - AMB_RS03255 0.63 -0.7 681,467 - AMB_RS03255 0.63 -0.0 681,467 - AMB_RS03255 0.63 -0.4 681,467 - AMB_RS03255 0.63 -0.1 681,467 - AMB_RS03255 0.63 -0.5 681,467 - AMB_RS03255 0.63 +0.1 681,467 - AMB_RS03255 0.63 -0.0 681,467 - AMB_RS03255 0.63 -0.7 681,467 - AMB_RS03255 0.63 +1.7 681,467 - AMB_RS03255 0.63 -0.9 681,467 - AMB_RS03255 0.63 -0.1 681,641 - AMB_RS03255 0.83 -1.4 681,664 + AMB_RS03255 0.86 -1.0 681,665 - AMB_RS03255 0.86 -0.4 681,665 - AMB_RS03255 0.86 -0.5 681,665 - AMB_RS03255 0.86 -0.7 681,757 + +0.9 681,758 - -0.2 681,758 - +0.6 681,758 - -0.3 681,758 - +0.2 681,784 + +0.5 681,784 + -0.4 681,784 + -0.3 681,784 + -0.1 681,784 + +1.9 681,785 - +0.5 681,785 - -0.3 681,785 - -0.5 681,785 - -0.8 681,785 - +0.0 681,785 - -0.1 681,785 - +2.4 681,786 + +0.2 681,786 + -0.9 681,786 + +0.2 681,786 + -0.1 681,787 - -0.8 681,787 - -0.3 681,787 - +0.8 681,787 - -0.6 681,787 - -0.3 681,787 - -0.4 681,886 + AMB_RS03260 0.25 -1.2 681,886 + AMB_RS03260 0.25 -0.3 681,977 - AMB_RS03260 0.50 -0.5 681,977 - AMB_RS03260 0.50 +0.4 681,977 - AMB_RS03260 0.50 +0.0 681,977 - AMB_RS03260 0.50 +0.4 681,977 - AMB_RS03260 0.50 +0.5 681,977 - AMB_RS03260 0.50 -1.4 681,977 - AMB_RS03260 0.50 -0.1 681,977 - AMB_RS03260 0.50 -0.0 682,005 + AMB_RS03260 0.58 +0.1 682,018 + AMB_RS03260 0.62 -0.0 682,070 - AMB_RS03260 0.76 -0.2 682,161 + -0.6 682,162 - -0.6 682,402 + AMB_RS03265 0.29 +0.0 682,402 + AMB_RS03265 0.29 -0.1 682,402 + AMB_RS03265 0.29 +0.1 682,402 + AMB_RS03265 0.29 -0.4 682,402 + AMB_RS03265 0.29 +0.9 682,402 + AMB_RS03265 0.29 +0.0 682,403 - AMB_RS03265 0.29 -0.4 682,403 - AMB_RS03265 0.29 -1.4 682,403 - AMB_RS03265 0.29 +0.4 682,403 - AMB_RS03265 0.29 +0.2 682,403 - AMB_RS03265 0.29 -0.0 682,403 - AMB_RS03265 0.29 -0.1 682,403 - AMB_RS03265 0.29 +0.1 682,403 - AMB_RS03265 0.29 -0.5 682,414 + AMB_RS03265 0.30 -1.2 682,414 + AMB_RS03265 0.30 -0.5 682,414 + AMB_RS03265 0.30 -0.4 682,414 + AMB_RS03265 0.30 -0.9 682,414 + AMB_RS03265 0.30 -1.2 682,414 + AMB_RS03265 0.30 +1.2 682,415 - AMB_RS03265 0.30 -0.2 682,415 - AMB_RS03265 0.30 -0.2 682,415 - AMB_RS03265 0.30 -0.1 682,415 - AMB_RS03265 0.30 -0.1 682,415 - AMB_RS03265 0.30 -0.3 683,025 + -0.2 683,342 + +0.2 683,356 + -1.8 683,356 + -0.6 683,356 + -0.6 683,357 - -0.9 683,359 + -0.5 683,676 + -0.1
Or see this region's nucleotide sequence