Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03200 and AMB_RS03205 are separated by 197 nucleotides AMB_RS03205 and AMB_RS03210 are separated by 435 nucleotides
AMB_RS03200: AMB_RS03200 - hypothetical protein, at 666,407 to 668,098
_RS03200
AMB_RS03205: AMB_RS03205 - DNA recombination/repair protein RecA, at 668,296 to 669,375
_RS03205
AMB_RS03210: AMB_RS03210 - DNA polymerase IV, at 669,811 to 670,992
_RS03210
Position (kb) 668
669
670 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 667.668 kb on + strand, within AMB_RS03200 at 667.669 kb on - strand, within AMB_RS03200 at 667.669 kb on - strand, within AMB_RS03200 at 667.764 kb on + strand, within AMB_RS03200 at 667.786 kb on - strand, within AMB_RS03200 at 667.786 kb on - strand, within AMB_RS03200 at 668.136 kb on + strand at 668.136 kb on + strand at 668.137 kb on - strand at 668.155 kb on + strand at 668.155 kb on + strand at 668.155 kb on + strand at 668.155 kb on + strand at 668.156 kb on - strand at 668.156 kb on - strand at 668.277 kb on + strand at 668.332 kb on - strand at 668.522 kb on - strand, within AMB_RS03205 at 669.119 kb on - strand, within AMB_RS03205 at 669.205 kb on + strand, within AMB_RS03205 at 669.205 kb on + strand, within AMB_RS03205 at 669.206 kb on - strand, within AMB_RS03205 at 669.373 kb on + strand at 669.374 kb on - strand at 669.380 kb on + strand at 669.513 kb on + strand at 669.513 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.578 kb on + strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.579 kb on - strand at 669.791 kb on + strand at 669.799 kb on + strand at 669.800 kb on - strand at 669.800 kb on - strand at 669.800 kb on - strand at 669.800 kb on - strand at 669.801 kb on + strand at 669.801 kb on + strand at 669.802 kb on - strand at 669.821 kb on - strand at 669.831 kb on + strand at 670.067 kb on - strand, within AMB_RS03210 at 670.067 kb on - strand, within AMB_RS03210 at 670.067 kb on - strand, within AMB_RS03210 at 670.067 kb on - strand, within AMB_RS03210 at 670.165 kb on + strand, within AMB_RS03210 at 670.165 kb on + strand, within AMB_RS03210 at 670.165 kb on + strand, within AMB_RS03210 at 670.165 kb on + strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.166 kb on - strand, within AMB_RS03210 at 670.246 kb on + strand, within AMB_RS03210 at 670.246 kb on + strand, within AMB_RS03210 at 670.247 kb on - strand, within AMB_RS03210 at 670.247 kb on - strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.363 kb on + strand, within AMB_RS03210 at 670.364 kb on - strand, within AMB_RS03210
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 667,668 + AMB_RS03200 0.75 +0.3 667,669 - AMB_RS03200 0.75 -1.2 667,669 - AMB_RS03200 0.75 -0.0 667,764 + AMB_RS03200 0.80 -0.3 667,786 - AMB_RS03200 0.82 -0.1 667,786 - AMB_RS03200 0.82 +0.2 668,136 + -0.5 668,136 + +0.7 668,137 - -0.1 668,155 + -0.3 668,155 + -0.4 668,155 + -0.6 668,155 + +0.1 668,156 - -0.9 668,156 - +0.1 668,277 + +0.3 668,332 - +0.1 668,522 - AMB_RS03205 0.21 -0.6 669,119 - AMB_RS03205 0.76 -0.6 669,205 + AMB_RS03205 0.84 -1.6 669,205 + AMB_RS03205 0.84 -3.3 669,206 - AMB_RS03205 0.84 -0.3 669,373 + +0.3 669,374 - -0.5 669,380 + -0.3 669,513 + -0.1 669,513 + +0.7 669,578 + +1.5 669,578 + -0.2 669,578 + +0.9 669,578 + -0.1 669,578 + -1.1 669,578 + +0.2 669,578 + +0.4 669,579 - -0.4 669,579 - -1.4 669,579 - +0.2 669,579 - -1.5 669,579 - -0.3 669,579 - +1.1 669,579 - +0.4 669,579 - +1.1 669,579 - +0.6 669,579 - -1.9 669,791 + +0.2 669,799 + -0.1 669,800 - -0.5 669,800 - -0.6 669,800 - -0.7 669,800 - +0.2 669,801 + -0.1 669,801 + -0.8 669,802 - +0.2 669,821 - -0.2 669,831 + -1.5 670,067 - AMB_RS03210 0.22 -1.1 670,067 - AMB_RS03210 0.22 +0.7 670,067 - AMB_RS03210 0.22 -0.2 670,067 - AMB_RS03210 0.22 +0.8 670,165 + AMB_RS03210 0.30 +0.2 670,165 + AMB_RS03210 0.30 +0.0 670,165 + AMB_RS03210 0.30 +1.4 670,165 + AMB_RS03210 0.30 +0.0 670,166 - AMB_RS03210 0.30 -0.4 670,166 - AMB_RS03210 0.30 +0.4 670,166 - AMB_RS03210 0.30 +0.7 670,166 - AMB_RS03210 0.30 -0.4 670,166 - AMB_RS03210 0.30 +0.1 670,246 + AMB_RS03210 0.37 -0.5 670,246 + AMB_RS03210 0.37 +0.4 670,247 - AMB_RS03210 0.37 -1.0 670,247 - AMB_RS03210 0.37 +0.5 670,363 + AMB_RS03210 0.47 -1.1 670,363 + AMB_RS03210 0.47 +0.7 670,363 + AMB_RS03210 0.47 -2.4 670,363 + AMB_RS03210 0.47 +0.4 670,363 + AMB_RS03210 0.47 -0.5 670,363 + AMB_RS03210 0.47 +0.1 670,364 - AMB_RS03210 0.47 -2.2
Or see this region's nucleotide sequence