Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03130 and AMB_RS03135 are separated by 248 nucleotides AMB_RS03135 and AMB_RS03140 are separated by 106 nucleotides
AMB_RS03130: AMB_RS03130 - LysM peptidoglycan-binding domain-containing protein, at 652,555 to 653,499
_RS03130
AMB_RS03135: AMB_RS03135 - NADP-dependent isocitrate dehydrogenase, at 653,748 to 655,973
_RS03135
AMB_RS03140: AMB_RS03140 - isocitrate dehydrogenase (NADP(+)), at 656,080 to 657,300
_RS03140
Position (kb) 653
654
655
656 Strain fitness (log2 ratio) -2
-1
0
1 at 652.905 kb on - strand, within AMB_RS03130 at 653.487 kb on + strand at 653.487 kb on + strand at 653.488 kb on - strand at 653.546 kb on + strand at 653.546 kb on + strand at 653.547 kb on - strand at 653.641 kb on - strand at 653.721 kb on + strand at 653.740 kb on + strand at 655.542 kb on + strand, within AMB_RS03135 at 655.734 kb on + strand, within AMB_RS03135 at 655.734 kb on + strand, within AMB_RS03135 at 655.734 kb on + strand, within AMB_RS03135 at 655.746 kb on + strand, within AMB_RS03135 at 655.746 kb on + strand, within AMB_RS03135 at 655.893 kb on + strand at 655.893 kb on + strand at 655.896 kb on + strand at 655.896 kb on + strand at 655.974 kb on + strand at 656.032 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 652,905 - AMB_RS03130 0.37 +0.2 653,487 + +0.8 653,487 + -0.6 653,488 - +0.3 653,546 + -0.3 653,546 + -0.1 653,547 - +0.3 653,641 - +0.5 653,721 + +0.4 653,740 + +0.1 655,542 + AMB_RS03135 0.81 -0.6 655,734 + AMB_RS03135 0.89 -2.3 655,734 + AMB_RS03135 0.89 -1.1 655,734 + AMB_RS03135 0.89 -0.8 655,746 + AMB_RS03135 0.90 -0.8 655,746 + AMB_RS03135 0.90 -0.7 655,893 + +0.4 655,893 + -0.7 655,896 + +0.5 655,896 + -0.4 655,974 + -0.3 656,032 + -0.5
Or see this region's nucleotide sequence