Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02695 and AMB_RS02700 are separated by 154 nucleotides AMB_RS02700 and AMB_RS02705 are separated by 95 nucleotides AMB_RS02705 and AMB_RS02710 are separated by 194 nucleotides
AMB_RS02695: AMB_RS02695 - undecaprenyl-diphosphatase 1, at 564,179 to 564,982
_RS02695
AMB_RS02700: AMB_RS02700 - carboxypeptidase, at 565,137 to 567,248
_RS02700
AMB_RS02705: AMB_RS02705 - ribbon-helix-helix protein, CopG family, at 567,344 to 567,547
_RS02705
AMB_RS02710: AMB_RS02710 - 2-nitropropane dioxygenase, at 567,742 to 569,139
_RS02710
Position (kb) 565
566
567
568 Strain fitness (log2 ratio) -1
0
1 at 564.144 kb on + strand at 564.144 kb on + strand at 564.144 kb on + strand at 564.145 kb on - strand at 564.145 kb on - strand at 564.145 kb on - strand at 564.145 kb on - strand at 564.145 kb on - strand at 564.189 kb on + strand at 564.189 kb on + strand at 564.190 kb on - strand at 564.351 kb on - strand, within AMB_RS02695 at 564.416 kb on + strand, within AMB_RS02695 at 564.646 kb on - strand, within AMB_RS02695 at 564.905 kb on - strand at 564.905 kb on - strand at 564.905 kb on - strand at 564.905 kb on - strand at 564.965 kb on + strand at 564.966 kb on - strand at 564.992 kb on - strand at 565.074 kb on + strand at 565.075 kb on - strand at 565.075 kb on - strand at 565.135 kb on + strand at 565.135 kb on + strand at 565.135 kb on + strand at 565.136 kb on - strand at 565.138 kb on + strand at 565.138 kb on + strand at 565.139 kb on - strand at 565.414 kb on + strand, within AMB_RS02700 at 565.414 kb on + strand, within AMB_RS02700 at 565.879 kb on + strand, within AMB_RS02700 at 565.891 kb on + strand, within AMB_RS02700 at 565.892 kb on - strand, within AMB_RS02700 at 565.978 kb on + strand, within AMB_RS02700 at 566.506 kb on + strand, within AMB_RS02700 at 566.506 kb on + strand, within AMB_RS02700 at 566.551 kb on + strand, within AMB_RS02700 at 566.566 kb on + strand, within AMB_RS02700 at 566.566 kb on + strand, within AMB_RS02700 at 566.739 kb on + strand, within AMB_RS02700 at 566.782 kb on + strand, within AMB_RS02700 at 566.969 kb on - strand, within AMB_RS02700 at 566.972 kb on - strand, within AMB_RS02700 at 567.259 kb on - strand at 567.259 kb on - strand at 567.308 kb on + strand at 567.308 kb on + strand at 567.309 kb on - strand at 567.309 kb on - strand at 567.309 kb on - strand at 567.309 kb on - strand at 567.309 kb on - strand at 567.329 kb on - strand at 567.455 kb on + strand, within AMB_RS02705 at 567.456 kb on - strand, within AMB_RS02705 at 567.456 kb on - strand, within AMB_RS02705 at 567.456 kb on - strand, within AMB_RS02705 at 567.456 kb on - strand, within AMB_RS02705 at 567.627 kb on + strand at 567.723 kb on - strand at 567.743 kb on + strand at 567.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 564,144 + +0.0 564,144 + -0.1 564,144 + -1.0 564,145 - -1.1 564,145 - -0.2 564,145 - -0.0 564,145 - +0.3 564,145 - -0.0 564,189 + -1.0 564,189 + -0.0 564,190 - +0.2 564,351 - AMB_RS02695 0.21 -0.4 564,416 + AMB_RS02695 0.29 -0.2 564,646 - AMB_RS02695 0.58 -0.4 564,905 - -0.0 564,905 - -1.0 564,905 - +0.1 564,905 - +0.1 564,965 + +0.5 564,966 - +0.5 564,992 - +0.2 565,074 + -1.0 565,075 - -0.5 565,075 - -0.8 565,135 + -0.0 565,135 + -0.3 565,135 + -0.2 565,136 - +0.1 565,138 + +0.6 565,138 + -0.0 565,139 - +0.3 565,414 + AMB_RS02700 0.13 -0.6 565,414 + AMB_RS02700 0.13 -1.0 565,879 + AMB_RS02700 0.35 -0.9 565,891 + AMB_RS02700 0.36 -0.0 565,892 - AMB_RS02700 0.36 +0.2 565,978 + AMB_RS02700 0.40 -1.2 566,506 + AMB_RS02700 0.65 -0.5 566,506 + AMB_RS02700 0.65 -1.1 566,551 + AMB_RS02700 0.67 -0.6 566,566 + AMB_RS02700 0.68 -0.6 566,566 + AMB_RS02700 0.68 -0.6 566,739 + AMB_RS02700 0.76 +0.0 566,782 + AMB_RS02700 0.78 -0.5 566,969 - AMB_RS02700 0.87 +0.4 566,972 - AMB_RS02700 0.87 -0.2 567,259 - -0.3 567,259 - +1.3 567,308 + -0.0 567,308 + -1.0 567,309 - +0.2 567,309 - -0.0 567,309 - -0.6 567,309 - -0.1 567,309 - -0.3 567,329 - +0.2 567,455 + AMB_RS02705 0.54 -0.4 567,456 - AMB_RS02705 0.55 +0.4 567,456 - AMB_RS02705 0.55 +0.0 567,456 - AMB_RS02705 0.55 +0.1 567,456 - AMB_RS02705 0.55 -0.2 567,627 + -0.2 567,723 - -0.3 567,743 + -0.0 567,744 - -0.7
Or see this region's nucleotide sequence