Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02550 and AMB_RS02555 are separated by 7 nucleotides AMB_RS02555 and AMB_RS02560 overlap by 4 nucleotides AMB_RS02560 and AMB_RS02565 are separated by 130 nucleotides
AMB_RS02550: AMB_RS02550 - DNA polymerase III, at 527,873 to 529,633
_RS02550
AMB_RS02555: AMB_RS02555 - cobyrinic acid a,c-diamide synthase, at 529,641 to 530,438
_RS02555
AMB_RS02560: AMB_RS02560 - GTP-binding protein, at 530,435 to 531,436
_RS02560
AMB_RS02565: AMB_RS02565 - flagellar biosynthesis protein FlhA, at 531,567 to 533,678
_RS02565
Position (kb) 530
531
532 Strain fitness (log2 ratio) -2
-1
0
1 at 529.642 kb on + strand at 529.828 kb on + strand, within AMB_RS02555 at 529.828 kb on + strand, within AMB_RS02555 at 529.828 kb on + strand, within AMB_RS02555 at 529.829 kb on - strand, within AMB_RS02555 at 529.829 kb on - strand, within AMB_RS02555 at 529.829 kb on - strand, within AMB_RS02555 at 529.924 kb on + strand, within AMB_RS02555 at 529.924 kb on + strand, within AMB_RS02555 at 529.924 kb on + strand, within AMB_RS02555 at 529.925 kb on - strand, within AMB_RS02555 at 529.925 kb on - strand, within AMB_RS02555 at 530.044 kb on + strand, within AMB_RS02555 at 530.044 kb on + strand, within AMB_RS02555 at 530.045 kb on - strand, within AMB_RS02555 at 530.773 kb on - strand, within AMB_RS02560 at 531.154 kb on + strand, within AMB_RS02560 at 531.154 kb on + strand, within AMB_RS02560 at 531.155 kb on - strand, within AMB_RS02560 at 531.427 kb on + strand at 531.427 kb on + strand at 531.427 kb on + strand at 531.427 kb on + strand at 531.428 kb on - strand at 531.428 kb on - strand at 531.428 kb on - strand at 531.428 kb on - strand at 531.428 kb on - strand at 531.428 kb on - strand at 531.667 kb on + strand at 531.667 kb on + strand at 531.929 kb on + strand, within AMB_RS02565 at 532.215 kb on - strand, within AMB_RS02565 at 532.215 kb on - strand, within AMB_RS02565 at 532.216 kb on + strand, within AMB_RS02565 at 532.216 kb on + strand, within AMB_RS02565 at 532.216 kb on + strand, within AMB_RS02565 at 532.216 kb on + strand, within AMB_RS02565 at 532.216 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.399 kb on + strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565 at 532.400 kb on - strand, within AMB_RS02565
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 529,642 + +0.1 529,828 + AMB_RS02555 0.23 -1.7 529,828 + AMB_RS02555 0.23 -0.0 529,828 + AMB_RS02555 0.23 -0.1 529,829 - AMB_RS02555 0.24 +0.2 529,829 - AMB_RS02555 0.24 +1.1 529,829 - AMB_RS02555 0.24 -0.3 529,924 + AMB_RS02555 0.35 +0.3 529,924 + AMB_RS02555 0.35 -0.0 529,924 + AMB_RS02555 0.35 -0.7 529,925 - AMB_RS02555 0.36 -0.0 529,925 - AMB_RS02555 0.36 -0.5 530,044 + AMB_RS02555 0.51 -0.3 530,044 + AMB_RS02555 0.51 -0.2 530,045 - AMB_RS02555 0.51 +0.5 530,773 - AMB_RS02560 0.34 -1.2 531,154 + AMB_RS02560 0.72 -0.8 531,154 + AMB_RS02560 0.72 -0.4 531,155 - AMB_RS02560 0.72 -0.3 531,427 + -0.3 531,427 + -0.7 531,427 + +0.7 531,427 + -0.5 531,428 - -0.3 531,428 - -1.3 531,428 - +0.0 531,428 - +1.2 531,428 - -0.2 531,428 - -0.1 531,667 + -2.0 531,667 + -0.1 531,929 + AMB_RS02565 0.17 -0.0 532,215 - AMB_RS02565 0.31 -0.5 532,215 - AMB_RS02565 0.31 +1.5 532,216 + AMB_RS02565 0.31 +0.1 532,216 + AMB_RS02565 0.31 +1.0 532,216 + AMB_RS02565 0.31 +0.7 532,216 + AMB_RS02565 0.31 -0.1 532,216 + AMB_RS02565 0.31 +1.1 532,399 + AMB_RS02565 0.39 -0.1 532,399 + AMB_RS02565 0.39 +0.1 532,399 + AMB_RS02565 0.39 -1.0 532,399 + AMB_RS02565 0.39 -1.2 532,399 + AMB_RS02565 0.39 -0.0 532,399 + AMB_RS02565 0.39 +1.0 532,399 + AMB_RS02565 0.39 +1.4 532,399 + AMB_RS02565 0.39 -0.6 532,399 + AMB_RS02565 0.39 +0.4 532,399 + AMB_RS02565 0.39 +0.9 532,399 + AMB_RS02565 0.39 -0.2 532,400 - AMB_RS02565 0.39 -0.3 532,400 - AMB_RS02565 0.39 +0.6 532,400 - AMB_RS02565 0.39 -0.6 532,400 - AMB_RS02565 0.39 -0.2 532,400 - AMB_RS02565 0.39 -0.9 532,400 - AMB_RS02565 0.39 +0.0 532,400 - AMB_RS02565 0.39 -0.3 532,400 - AMB_RS02565 0.39 -0.4 532,400 - AMB_RS02565 0.39 -0.3 532,400 - AMB_RS02565 0.39 +1.2 532,400 - AMB_RS02565 0.39 -0.2
Or see this region's nucleotide sequence