Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23890 and AMB_RS02500 are separated by 57 nucleotides AMB_RS02500 and AMB_RS02505 overlap by 4 nucleotides AMB_RS02505 and AMB_RS02510 are separated by 512 nucleotides AMB_RS02510 and AMB_RS02515 are separated by 222 nucleotides AMB_RS02515 and AMB_RS02520 are separated by 48 nucleotides
AMB_RS23890: AMB_RS23890 - hypothetical protein, at 517,361 to 518,557
_RS23890
AMB_RS02500: AMB_RS02500 - hypothetical protein, at 518,615 to 518,851
_RS02500
AMB_RS02505: AMB_RS02505 - hypothetical protein, at 518,848 to 519,033
_RS02505
AMB_RS02510: AMB_RS02510 - DUF4102 domain-containing protein, at 519,546 to 520,784
_RS02510
AMB_RS02515: AMB_RS02515 - tRNA-Ala, at 521,007 to 521,082
_RS02515
AMB_RS02520: AMB_RS02520 - hypothetical protein, at 521,131 to 522,141
_RS02520
Position (kb) 519
520
521 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 518.585 kb on + strand at 518.616 kb on + strand at 518.655 kb on + strand, within AMB_RS02500 at 518.655 kb on + strand, within AMB_RS02500 at 518.655 kb on + strand, within AMB_RS02500 at 518.700 kb on - strand, within AMB_RS02500 at 518.700 kb on - strand, within AMB_RS02500 at 518.700 kb on - strand, within AMB_RS02500 at 518.700 kb on - strand, within AMB_RS02500 at 518.892 kb on + strand, within AMB_RS02505 at 518.892 kb on + strand, within AMB_RS02505 at 518.892 kb on + strand, within AMB_RS02505 at 518.893 kb on - strand, within AMB_RS02505 at 518.895 kb on - strand, within AMB_RS02505 at 518.949 kb on + strand, within AMB_RS02505 at 518.950 kb on - strand, within AMB_RS02505 at 518.957 kb on + strand, within AMB_RS02505 at 519.008 kb on - strand, within AMB_RS02505 at 519.077 kb on + strand at 519.078 kb on - strand at 519.078 kb on - strand at 519.078 kb on - strand at 519.078 kb on - strand at 519.085 kb on + strand at 519.085 kb on + strand at 519.124 kb on - strand at 519.286 kb on + strand at 519.287 kb on - strand at 519.287 kb on - strand at 519.468 kb on - strand at 519.499 kb on + strand at 519.499 kb on + strand at 519.499 kb on + strand at 519.500 kb on - strand at 519.500 kb on - strand at 519.536 kb on - strand at 519.536 kb on - strand at 519.585 kb on + strand at 519.617 kb on - strand at 519.617 kb on - strand at 519.658 kb on + strand at 519.658 kb on + strand at 519.658 kb on + strand at 519.659 kb on - strand at 519.659 kb on - strand at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.811 kb on + strand, within AMB_RS02510 at 519.837 kb on + strand, within AMB_RS02510 at 519.945 kb on + strand, within AMB_RS02510 at 520.046 kb on + strand, within AMB_RS02510 at 520.046 kb on + strand, within AMB_RS02510 at 520.046 kb on + strand, within AMB_RS02510 at 520.047 kb on - strand, within AMB_RS02510 at 520.047 kb on - strand, within AMB_RS02510 at 520.047 kb on - strand, within AMB_RS02510 at 520.047 kb on - strand, within AMB_RS02510 at 520.087 kb on - strand, within AMB_RS02510 at 520.087 kb on - strand, within AMB_RS02510 at 520.087 kb on - strand, within AMB_RS02510 at 520.087 kb on - strand, within AMB_RS02510 at 520.255 kb on + strand, within AMB_RS02510 at 520.262 kb on + strand, within AMB_RS02510 at 520.263 kb on - strand, within AMB_RS02510 at 520.263 kb on - strand, within AMB_RS02510 at 520.263 kb on - strand, within AMB_RS02510 at 520.263 kb on - strand, within AMB_RS02510 at 520.289 kb on + strand, within AMB_RS02510 at 520.355 kb on + strand, within AMB_RS02510 at 520.356 kb on - strand, within AMB_RS02510 at 520.356 kb on - strand, within AMB_RS02510 at 520.457 kb on + strand, within AMB_RS02510 at 520.458 kb on - strand, within AMB_RS02510 at 520.458 kb on - strand, within AMB_RS02510 at 520.560 kb on + strand, within AMB_RS02510 at 520.561 kb on - strand, within AMB_RS02510 at 520.661 kb on - strand at 520.675 kb on + strand at 520.676 kb on - strand at 520.732 kb on - strand at 520.732 kb on - strand at 520.732 kb on - strand at 520.760 kb on + strand at 520.793 kb on - strand at 520.814 kb on - strand at 520.814 kb on - strand at 520.827 kb on + strand at 520.827 kb on + strand at 520.827 kb on + strand at 520.827 kb on + strand at 520.828 kb on - strand at 520.828 kb on - strand at 520.828 kb on - strand at 520.899 kb on + strand at 520.900 kb on - strand at 520.900 kb on - strand at 520.949 kb on - strand at 521.214 kb on - strand at 521.282 kb on - strand, within AMB_RS02520 at 521.357 kb on + strand, within AMB_RS02520 at 521.357 kb on + strand, within AMB_RS02520 at 521.358 kb on - strand, within AMB_RS02520 at 521.358 kb on - strand, within AMB_RS02520 at 521.429 kb on + strand, within AMB_RS02520 at 521.429 kb on + strand, within AMB_RS02520 at 521.429 kb on + strand, within AMB_RS02520 at 521.430 kb on - strand, within AMB_RS02520 at 521.510 kb on + strand, within AMB_RS02520 at 521.510 kb on + strand, within AMB_RS02520 at 521.510 kb on + strand, within AMB_RS02520 at 521.511 kb on - strand, within AMB_RS02520 at 521.511 kb on - strand, within AMB_RS02520 at 521.511 kb on - strand, within AMB_RS02520 at 521.583 kb on + strand, within AMB_RS02520 at 521.585 kb on + strand, within AMB_RS02520 at 521.586 kb on - strand, within AMB_RS02520 at 521.615 kb on + strand, within AMB_RS02520 at 521.615 kb on + strand, within AMB_RS02520
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 518,585 + +0.1 518,616 + +0.1 518,655 + AMB_RS02500 0.17 +0.4 518,655 + AMB_RS02500 0.17 +0.5 518,655 + AMB_RS02500 0.17 -2.3 518,700 - AMB_RS02500 0.36 +1.6 518,700 - AMB_RS02500 0.36 -2.2 518,700 - AMB_RS02500 0.36 -1.6 518,700 - AMB_RS02500 0.36 -0.0 518,892 + AMB_RS02505 0.24 -0.4 518,892 + AMB_RS02505 0.24 -0.0 518,892 + AMB_RS02505 0.24 +0.2 518,893 - AMB_RS02505 0.24 +0.3 518,895 - AMB_RS02505 0.25 -1.5 518,949 + AMB_RS02505 0.54 -1.0 518,950 - AMB_RS02505 0.55 -0.3 518,957 + AMB_RS02505 0.59 +1.6 519,008 - AMB_RS02505 0.86 -0.4 519,077 + +2.4 519,078 - -0.2 519,078 - -1.7 519,078 - -2.6 519,078 - -0.2 519,085 + +0.2 519,085 + +0.4 519,124 - +0.3 519,286 + -0.5 519,287 - -1.4 519,287 - -0.3 519,468 - -0.9 519,499 + -2.0 519,499 + -0.4 519,499 + -1.2 519,500 - -1.6 519,500 - +0.3 519,536 - -0.3 519,536 - -0.2 519,585 + -0.3 519,617 - -0.6 519,617 - -1.9 519,658 + +0.0 519,658 + +0.1 519,658 + -0.1 519,659 - -0.2 519,659 - +1.0 519,674 - AMB_RS02510 0.10 -0.1 519,674 - AMB_RS02510 0.10 -0.5 519,674 - AMB_RS02510 0.10 +0.5 519,674 - AMB_RS02510 0.10 -3.0 519,674 - AMB_RS02510 0.10 -0.2 519,674 - AMB_RS02510 0.10 -0.2 519,811 + AMB_RS02510 0.21 -1.0 519,837 + AMB_RS02510 0.23 +0.3 519,945 + AMB_RS02510 0.32 -2.7 520,046 + AMB_RS02510 0.40 -0.2 520,046 + AMB_RS02510 0.40 +0.5 520,046 + AMB_RS02510 0.40 -0.4 520,047 - AMB_RS02510 0.40 -0.2 520,047 - AMB_RS02510 0.40 -0.7 520,047 - AMB_RS02510 0.40 +1.6 520,047 - AMB_RS02510 0.40 -0.2 520,087 - AMB_RS02510 0.44 -0.6 520,087 - AMB_RS02510 0.44 -0.4 520,087 - AMB_RS02510 0.44 -0.1 520,087 - AMB_RS02510 0.44 -0.6 520,255 + AMB_RS02510 0.57 -0.3 520,262 + AMB_RS02510 0.58 -0.2 520,263 - AMB_RS02510 0.58 -0.6 520,263 - AMB_RS02510 0.58 -0.0 520,263 - AMB_RS02510 0.58 +0.3 520,263 - AMB_RS02510 0.58 -1.5 520,289 + AMB_RS02510 0.60 -0.5 520,355 + AMB_RS02510 0.65 -0.5 520,356 - AMB_RS02510 0.65 -0.0 520,356 - AMB_RS02510 0.65 -1.2 520,457 + AMB_RS02510 0.74 -1.7 520,458 - AMB_RS02510 0.74 +0.5 520,458 - AMB_RS02510 0.74 -0.0 520,560 + AMB_RS02510 0.82 -0.1 520,561 - AMB_RS02510 0.82 -0.0 520,661 - -1.2 520,675 + -0.6 520,676 - +0.7 520,732 - -1.7 520,732 - -1.6 520,732 - -0.2 520,760 + -0.1 520,793 - -0.6 520,814 - -0.1 520,814 - -0.1 520,827 + +0.3 520,827 + -0.0 520,827 + -0.1 520,827 + +0.4 520,828 - -0.3 520,828 - -0.3 520,828 - -0.2 520,899 + -0.2 520,900 - -0.6 520,900 - -0.2 520,949 - +0.2 521,214 - -0.5 521,282 - AMB_RS02520 0.15 -1.9 521,357 + AMB_RS02520 0.22 -0.2 521,357 + AMB_RS02520 0.22 -0.3 521,358 - AMB_RS02520 0.22 -0.6 521,358 - AMB_RS02520 0.22 -0.7 521,429 + AMB_RS02520 0.29 +0.1 521,429 + AMB_RS02520 0.29 -0.0 521,429 + AMB_RS02520 0.29 +0.1 521,430 - AMB_RS02520 0.30 -0.9 521,510 + AMB_RS02520 0.37 +0.3 521,510 + AMB_RS02520 0.37 -0.4 521,510 + AMB_RS02520 0.37 -0.2 521,511 - AMB_RS02520 0.38 -0.8 521,511 - AMB_RS02520 0.38 +0.1 521,511 - AMB_RS02520 0.38 -1.4 521,583 + AMB_RS02520 0.45 -1.9 521,585 + AMB_RS02520 0.45 -0.8 521,586 - AMB_RS02520 0.45 -1.7 521,615 + AMB_RS02520 0.48 -0.2 521,615 + AMB_RS02520 0.48 +0.5
Or see this region's nucleotide sequence