Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01845 and AMB_RS01850 overlap by 14 nucleotides AMB_RS01850 and AMB_RS01855 are separated by 258 nucleotides AMB_RS01855 and AMB_RS01860 are separated by 90 nucleotides AMB_RS01860 and AMB_RS01865 are separated by 3 nucleotides AMB_RS01865 and AMB_RS01870 overlap by 25 nucleotides
AMB_RS01845: AMB_RS01845 - DNA methylase, at 397,748 to 399,019
_RS01845
AMB_RS01850: AMB_RS01850 - hypothetical protein, at 399,006 to 399,221
_RS01850
AMB_RS01855: AMB_RS01855 - hypothetical protein, at 399,480 to 399,689
_RS01855
AMB_RS01860: AMB_RS01860 - HU family DNA-binding protein, at 399,780 to 400,034
_RS01860
AMB_RS01865: AMB_RS01865 - hypothetical protein, at 400,038 to 400,226
_RS01865
AMB_RS01870: AMB_RS01870 - DUF3489 domain-containing protein, at 400,202 to 400,792
_RS01870
Position (kb) 399
400 Strain fitness (log2 ratio) -2
-1
0
1 at 398.495 kb on + strand, within AMB_RS01845 at 398.495 kb on + strand, within AMB_RS01845 at 398.496 kb on - strand, within AMB_RS01845 at 398.496 kb on - strand, within AMB_RS01845 at 398.496 kb on - strand, within AMB_RS01845 at 398.615 kb on + strand, within AMB_RS01845 at 398.642 kb on + strand, within AMB_RS01845 at 398.642 kb on + strand, within AMB_RS01845 at 398.642 kb on + strand, within AMB_RS01845 at 398.642 kb on + strand, within AMB_RS01845 at 398.643 kb on - strand, within AMB_RS01845 at 398.903 kb on + strand at 398.904 kb on - strand at 398.904 kb on - strand at 399.040 kb on + strand, within AMB_RS01850 at 399.053 kb on + strand, within AMB_RS01850 at 399.054 kb on - strand, within AMB_RS01850 at 399.127 kb on + strand, within AMB_RS01850 at 399.128 kb on - strand, within AMB_RS01850 at 399.145 kb on + strand, within AMB_RS01850 at 399.426 kb on + strand at 399.427 kb on - strand at 399.469 kb on - strand at 399.537 kb on + strand, within AMB_RS01855 at 399.568 kb on + strand, within AMB_RS01855 at 399.568 kb on + strand, within AMB_RS01855 at 399.654 kb on + strand, within AMB_RS01855 at 399.654 kb on + strand, within AMB_RS01855 at 399.655 kb on - strand, within AMB_RS01855 at 399.655 kb on - strand, within AMB_RS01855 at 399.685 kb on + strand at 399.685 kb on + strand at 399.685 kb on + strand at 399.686 kb on - strand at 399.686 kb on - strand at 399.696 kb on + strand at 399.729 kb on + strand at 399.729 kb on + strand at 399.729 kb on + strand at 399.770 kb on + strand at 399.770 kb on + strand at 399.771 kb on - strand at 400.032 kb on + strand at 400.032 kb on + strand at 400.033 kb on - strand at 400.092 kb on + strand, within AMB_RS01865 at 400.139 kb on - strand, within AMB_RS01865 at 400.324 kb on + strand, within AMB_RS01870 at 400.324 kb on + strand, within AMB_RS01870 at 400.324 kb on + strand, within AMB_RS01870 at 400.430 kb on - strand, within AMB_RS01870 at 400.430 kb on - strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 398,495 + AMB_RS01845 0.59 -0.1 398,495 + AMB_RS01845 0.59 +0.1 398,496 - AMB_RS01845 0.59 -1.0 398,496 - AMB_RS01845 0.59 -0.2 398,496 - AMB_RS01845 0.59 -0.9 398,615 + AMB_RS01845 0.68 +0.4 398,642 + AMB_RS01845 0.70 -0.1 398,642 + AMB_RS01845 0.70 +0.3 398,642 + AMB_RS01845 0.70 -0.8 398,642 + AMB_RS01845 0.70 +0.3 398,643 - AMB_RS01845 0.70 -1.2 398,903 + -0.2 398,904 - +0.3 398,904 - -0.4 399,040 + AMB_RS01850 0.16 +0.6 399,053 + AMB_RS01850 0.22 -0.4 399,054 - AMB_RS01850 0.22 +0.1 399,127 + AMB_RS01850 0.56 +0.0 399,128 - AMB_RS01850 0.56 -0.5 399,145 + AMB_RS01850 0.64 -0.2 399,426 + +0.0 399,427 - -0.0 399,469 - -0.3 399,537 + AMB_RS01855 0.27 -0.3 399,568 + AMB_RS01855 0.42 -1.0 399,568 + AMB_RS01855 0.42 -1.3 399,654 + AMB_RS01855 0.83 -0.2 399,654 + AMB_RS01855 0.83 -0.1 399,655 - AMB_RS01855 0.83 -0.7 399,655 - AMB_RS01855 0.83 -1.1 399,685 + +0.5 399,685 + +1.1 399,685 + +0.6 399,686 - -0.4 399,686 - -0.6 399,696 + +0.1 399,729 + -2.2 399,729 + -1.7 399,729 + -1.6 399,770 + -0.4 399,770 + -1.0 399,771 - -1.3 400,032 + +0.7 400,032 + -1.1 400,033 - +1.3 400,092 + AMB_RS01865 0.29 -0.2 400,139 - AMB_RS01865 0.53 -0.5 400,324 + AMB_RS01870 0.21 +0.1 400,324 + AMB_RS01870 0.21 +0.6 400,324 + AMB_RS01870 0.21 -1.0 400,430 - AMB_RS01870 0.39 +0.2 400,430 - AMB_RS01870 0.39 -0.6 400,554 + AMB_RS01870 0.60 -0.0 400,554 + AMB_RS01870 0.60 -0.6 400,554 + AMB_RS01870 0.60 -0.1 400,554 + AMB_RS01870 0.60 +0.6 400,554 + AMB_RS01870 0.60 +0.8 400,555 - AMB_RS01870 0.60 +0.7 400,555 - AMB_RS01870 0.60 -0.3 400,555 - AMB_RS01870 0.60 +0.1 400,555 - AMB_RS01870 0.60 -0.9 400,555 - AMB_RS01870 0.60 +0.1
Or see this region's nucleotide sequence