Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01605 and AMB_RS01610 overlap by 4 nucleotides AMB_RS01610 and AMB_RS01615 overlap by 13 nucleotides AMB_RS01615 and AMB_RS01620 are separated by 61 nucleotides AMB_RS01620 and AMB_RS01625 are separated by 168 nucleotides
AMB_RS01605: AMB_RS01605 - aspartate aminotransferase family protein, at 356,586 to 357,740
_RS01605
AMB_RS01610: AMB_RS01610 - ornithine carbamoyltransferase, at 357,737 to 358,654
_RS01610
AMB_RS01615: AMB_RS01615 - molecular chaperone, at 358,642 to 359,550
_RS01615
AMB_RS01620: AMB_RS01620 - hypothetical protein, at 359,612 to 360,205
_RS01620
AMB_RS01625: AMB_RS01625 - 50S ribosomal protein L28, at 360,374 to 360,679
_RS01625
Position (kb) 358
359
360 Strain fitness (log2 ratio) -1
0
1 at 357.647 kb on + strand at 357.647 kb on + strand at 359.000 kb on - strand, within AMB_RS01615 at 359.000 kb on - strand, within AMB_RS01615 at 359.000 kb on - strand, within AMB_RS01615 at 359.000 kb on - strand, within AMB_RS01615 at 359.049 kb on + strand, within AMB_RS01615 at 359.050 kb on - strand, within AMB_RS01615 at 359.095 kb on + strand, within AMB_RS01615 at 359.096 kb on - strand, within AMB_RS01615 at 359.336 kb on - strand, within AMB_RS01615 at 359.344 kb on + strand, within AMB_RS01615 at 359.344 kb on + strand, within AMB_RS01615 at 359.344 kb on + strand, within AMB_RS01615 at 359.345 kb on - strand, within AMB_RS01615 at 360.183 kb on - strand at 360.203 kb on + strand at 360.203 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 357,647 + +0.4 357,647 + -1.4 359,000 - AMB_RS01615 0.39 +0.1 359,000 - AMB_RS01615 0.39 -0.0 359,000 - AMB_RS01615 0.39 +0.4 359,000 - AMB_RS01615 0.39 +0.9 359,049 + AMB_RS01615 0.45 +0.1 359,050 - AMB_RS01615 0.45 +0.6 359,095 + AMB_RS01615 0.50 -0.3 359,096 - AMB_RS01615 0.50 -0.9 359,336 - AMB_RS01615 0.76 -0.8 359,344 + AMB_RS01615 0.77 -0.0 359,344 + AMB_RS01615 0.77 -0.4 359,344 + AMB_RS01615 0.77 +0.4 359,345 - AMB_RS01615 0.77 +0.6 360,183 - -1.6 360,203 + -0.7 360,203 + -0.6
Or see this region's nucleotide sequence