Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01345 and AMB_RS01350 overlap by 73 nucleotides AMB_RS01350 and AMB_RS01355 are separated by 221 nucleotides
AMB_RS01345: AMB_RS01345 - glycosyltransferase family 1 protein, at 297,090 to 298,133
_RS01345
AMB_RS01350: AMB_RS01350 - UDP-2,3-diacylglucosamine diphosphatase, at 298,061 to 298,897
_RS01350
AMB_RS01355: AMB_RS01355 - sphingosine kinase, at 299,119 to 300,000
_RS01355
Position (kb) 298
299 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 297.086 kb on + strand at 297.087 kb on - strand at 297.132 kb on + strand at 297.133 kb on - strand at 297.302 kb on - strand, within AMB_RS01345 at 297.398 kb on - strand, within AMB_RS01345 at 297.671 kb on + strand, within AMB_RS01345 at 297.766 kb on + strand, within AMB_RS01345 at 297.766 kb on + strand, within AMB_RS01345 at 297.767 kb on - strand, within AMB_RS01345 at 297.767 kb on - strand, within AMB_RS01345 at 297.805 kb on + strand, within AMB_RS01345 at 297.806 kb on - strand, within AMB_RS01345 at 297.806 kb on - strand, within AMB_RS01345 at 297.826 kb on + strand, within AMB_RS01345 at 297.826 kb on + strand, within AMB_RS01345 at 297.826 kb on + strand, within AMB_RS01345 at 297.827 kb on - strand, within AMB_RS01345 at 298.125 kb on + strand at 298.125 kb on + strand at 298.125 kb on + strand at 298.125 kb on + strand at 298.125 kb on + strand at 298.233 kb on + strand, within AMB_RS01350 at 298.234 kb on - strand, within AMB_RS01350 at 298.234 kb on - strand, within AMB_RS01350 at 298.347 kb on + strand, within AMB_RS01350 at 298.347 kb on + strand, within AMB_RS01350 at 298.347 kb on + strand, within AMB_RS01350 at 298.347 kb on + strand, within AMB_RS01350 at 298.348 kb on - strand, within AMB_RS01350 at 298.348 kb on - strand, within AMB_RS01350 at 298.348 kb on - strand, within AMB_RS01350 at 298.348 kb on - strand, within AMB_RS01350 at 298.348 kb on - strand, within AMB_RS01350 at 298.461 kb on + strand, within AMB_RS01350 at 298.461 kb on + strand, within AMB_RS01350 at 298.461 kb on + strand, within AMB_RS01350 at 298.461 kb on + strand, within AMB_RS01350 at 298.462 kb on - strand, within AMB_RS01350 at 298.462 kb on - strand, within AMB_RS01350 at 298.497 kb on + strand, within AMB_RS01350 at 298.497 kb on + strand, within AMB_RS01350 at 298.768 kb on - strand, within AMB_RS01350 at 298.768 kb on - strand, within AMB_RS01350 at 298.768 kb on - strand, within AMB_RS01350 at 298.940 kb on - strand at 299.035 kb on - strand at 299.057 kb on - strand at 299.605 kb on + strand, within AMB_RS01355 at 299.606 kb on - strand, within AMB_RS01355 at 299.606 kb on - strand, within AMB_RS01355 at 299.606 kb on - strand, within AMB_RS01355 at 299.681 kb on - strand, within AMB_RS01355 at 299.719 kb on + strand, within AMB_RS01355 at 299.719 kb on + strand, within AMB_RS01355 at 299.719 kb on + strand, within AMB_RS01355
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 297,086 + +0.3 297,087 - -0.8 297,132 + -0.8 297,133 - -0.2 297,302 - AMB_RS01345 0.20 +0.8 297,398 - AMB_RS01345 0.30 +0.8 297,671 + AMB_RS01345 0.56 +0.6 297,766 + AMB_RS01345 0.65 -0.1 297,766 + AMB_RS01345 0.65 +0.1 297,767 - AMB_RS01345 0.65 +0.7 297,767 - AMB_RS01345 0.65 +0.3 297,805 + AMB_RS01345 0.68 +0.5 297,806 - AMB_RS01345 0.69 -0.4 297,806 - AMB_RS01345 0.69 +0.2 297,826 + AMB_RS01345 0.70 -2.0 297,826 + AMB_RS01345 0.70 -3.2 297,826 + AMB_RS01345 0.70 +0.6 297,827 - AMB_RS01345 0.71 -0.3 298,125 + -0.5 298,125 + -0.1 298,125 + -0.3 298,125 + +0.1 298,125 + +0.1 298,233 + AMB_RS01350 0.21 -0.7 298,234 - AMB_RS01350 0.21 +0.9 298,234 - AMB_RS01350 0.21 -1.6 298,347 + AMB_RS01350 0.34 -1.3 298,347 + AMB_RS01350 0.34 +1.0 298,347 + AMB_RS01350 0.34 -1.0 298,347 + AMB_RS01350 0.34 +0.1 298,348 - AMB_RS01350 0.34 +0.4 298,348 - AMB_RS01350 0.34 -0.4 298,348 - AMB_RS01350 0.34 +0.1 298,348 - AMB_RS01350 0.34 -0.2 298,348 - AMB_RS01350 0.34 -0.5 298,461 + AMB_RS01350 0.48 +0.3 298,461 + AMB_RS01350 0.48 -0.0 298,461 + AMB_RS01350 0.48 -1.0 298,461 + AMB_RS01350 0.48 -1.6 298,462 - AMB_RS01350 0.48 -0.7 298,462 - AMB_RS01350 0.48 -1.3 298,497 + AMB_RS01350 0.52 -0.6 298,497 + AMB_RS01350 0.52 -0.0 298,768 - AMB_RS01350 0.84 +0.1 298,768 - AMB_RS01350 0.84 -0.4 298,768 - AMB_RS01350 0.84 -1.3 298,940 - +0.2 299,035 - +0.9 299,057 - -0.2 299,605 + AMB_RS01355 0.55 -0.5 299,606 - AMB_RS01355 0.55 -0.7 299,606 - AMB_RS01355 0.55 -0.6 299,606 - AMB_RS01355 0.55 +0.5 299,681 - AMB_RS01355 0.64 +0.0 299,719 + AMB_RS01355 0.68 +1.8 299,719 + AMB_RS01355 0.68 -0.8 299,719 + AMB_RS01355 0.68 -0.2
Or see this region's nucleotide sequence