Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_20270 and IAI46_20275 are separated by 43 nucleotides IAI46_20275 and IAI46_20280 are separated by 128 nucleotides IAI46_20280 and IAI46_20285 are separated by 95 nucleotides IAI46_20285 and IAI46_20290 are separated by 33 nucleotides
IAI46_20270: IAI46_20270 - bifunctional uridylyltransferase/uridylyl-removing protein GlnD, at 4,276,809 to 4,279,487
_20270
IAI46_20275: IAI46_20275 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 4,279,531 to 4,280,355
_20275
IAI46_20280: IAI46_20280 - DUF3461 family protein, at 4,280,484 to 4,280,873
_20280
IAI46_20285: IAI46_20285 - flavodoxin, at 4,280,969 to 4,281,418
_20285
IAI46_20290: IAI46_20290 - tRNA pseudouridine(65) synthase TruC, at 4,281,452 to 4,282,222
_20290
Position (kb)
4280
4281 Strain fitness (log2 ratio)
-1
0
1 at 4279.488 kb on + strand at 4279.495 kb on - strand at 4280.358 kb on + strand at 4280.358 kb on + strand at 4280.359 kb on - strand at 4280.399 kb on + strand at 4280.400 kb on - strand at 4280.400 kb on - strand at 4280.429 kb on + strand at 4280.456 kb on + strand at 4280.464 kb on - strand at 4280.467 kb on + strand at 4280.470 kb on - strand at 4280.481 kb on - strand at 4280.483 kb on + strand at 4280.483 kb on + strand at 4280.483 kb on + strand at 4280.483 kb on + strand at 4280.484 kb on - strand at 4280.484 kb on - strand at 4280.484 kb on - strand at 4280.484 kb on - strand at 4280.484 kb on - strand at 4280.484 kb on - strand at 4280.487 kb on + strand at 4280.487 kb on + strand at 4280.488 kb on - strand at 4280.540 kb on + strand, within IAI46_20280 at 4280.541 kb on - strand, within IAI46_20280 at 4280.541 kb on - strand, within IAI46_20280 at 4280.541 kb on - strand, within IAI46_20280 at 4280.581 kb on - strand, within IAI46_20280 at 4280.679 kb on + strand, within IAI46_20280 at 4280.679 kb on + strand, within IAI46_20280 at 4280.715 kb on + strand, within IAI46_20280 at 4280.715 kb on + strand, within IAI46_20280 at 4280.716 kb on - strand, within IAI46_20280 at 4280.716 kb on - strand, within IAI46_20280 at 4280.868 kb on - strand at 4280.871 kb on + strand at 4280.872 kb on - strand at 4280.872 kb on - strand at 4280.894 kb on - strand at 4280.909 kb on + strand at 4280.910 kb on - strand at 4280.934 kb on + strand at 4281.062 kb on + strand, within IAI46_20285 at 4281.130 kb on - strand, within IAI46_20285 at 4281.196 kb on + strand, within IAI46_20285 at 4281.197 kb on - strand, within IAI46_20285 at 4281.197 kb on - strand, within IAI46_20285 at 4281.253 kb on + strand, within IAI46_20285 at 4281.253 kb on + strand, within IAI46_20285 at 4281.253 kb on + strand, within IAI46_20285 at 4281.254 kb on - strand, within IAI46_20285 at 4281.254 kb on - strand, within IAI46_20285 at 4281.257 kb on + strand, within IAI46_20285 at 4281.259 kb on - strand, within IAI46_20285 at 4281.267 kb on + strand, within IAI46_20285 at 4281.279 kb on + strand, within IAI46_20285 at 4281.279 kb on + strand, within IAI46_20285 at 4281.425 kb on + strand at 4281.434 kb on - strand at 4281.434 kb on - strand at 4281.437 kb on + strand at 4281.437 kb on + strand at 4281.438 kb on - strand at 4281.443 kb on + strand at 4281.469 kb on + strand at 4281.532 kb on + strand, within IAI46_20290 at 4281.532 kb on + strand, within IAI46_20290 at 4281.532 kb on + strand, within IAI46_20290 at 4281.532 kb on + strand, within IAI46_20290 at 4281.533 kb on - strand, within IAI46_20290 at 4281.533 kb on - strand, within IAI46_20290 at 4281.533 kb on - strand, within IAI46_20290 at 4281.712 kb on + strand, within IAI46_20290 at 4281.721 kb on + strand, within IAI46_20290 at 4281.722 kb on - strand, within IAI46_20290 at 4281.722 kb on - strand, within IAI46_20290
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 4,279,488 + -1.1 4,279,495 - +1.5 4,280,358 + +0.5 4,280,358 + -1.4 4,280,359 - +0.7 4,280,399 + -0.1 4,280,400 - -0.3 4,280,400 - +1.0 4,280,429 + -0.5 4,280,456 + +0.1 4,280,464 - +0.6 4,280,467 + +0.7 4,280,470 - +0.1 4,280,481 - -0.1 4,280,483 + -0.3 4,280,483 + +0.4 4,280,483 + +0.4 4,280,483 + -0.9 4,280,484 - -0.6 4,280,484 - -0.7 4,280,484 - +1.4 4,280,484 - +1.0 4,280,484 - -1.0 4,280,484 - +0.3 4,280,487 + +0.4 4,280,487 + -0.6 4,280,488 - +1.3 4,280,540 + IAI46_20280 0.14 -0.1 4,280,541 - IAI46_20280 0.15 +0.7 4,280,541 - IAI46_20280 0.15 -1.0 4,280,541 - IAI46_20280 0.15 +0.5 4,280,581 - IAI46_20280 0.25 -0.2 4,280,679 + IAI46_20280 0.50 +0.5 4,280,679 + IAI46_20280 0.50 -1.0 4,280,715 + IAI46_20280 0.59 -0.8 4,280,715 + IAI46_20280 0.59 +0.2 4,280,716 - IAI46_20280 0.59 -0.2 4,280,716 - IAI46_20280 0.59 +0.4 4,280,868 - -0.3 4,280,871 + +0.3 4,280,872 - -0.5 4,280,872 - +0.3 4,280,894 - +0.3 4,280,909 + -0.1 4,280,910 - -0.3 4,280,934 + +0.7 4,281,062 + IAI46_20285 0.21 +0.7 4,281,130 - IAI46_20285 0.36 +0.5 4,281,196 + IAI46_20285 0.50 -0.1 4,281,197 - IAI46_20285 0.51 -0.3 4,281,197 - IAI46_20285 0.51 +0.7 4,281,253 + IAI46_20285 0.63 -0.1 4,281,253 + IAI46_20285 0.63 -0.1 4,281,253 + IAI46_20285 0.63 -0.8 4,281,254 - IAI46_20285 0.63 -0.1 4,281,254 - IAI46_20285 0.63 -0.4 4,281,257 + IAI46_20285 0.64 +0.8 4,281,259 - IAI46_20285 0.64 +0.3 4,281,267 + IAI46_20285 0.66 -1.3 4,281,279 + IAI46_20285 0.69 +0.7 4,281,279 + IAI46_20285 0.69 +0.0 4,281,425 + +0.1 4,281,434 - -0.1 4,281,434 - -0.9 4,281,437 + -0.7 4,281,437 + -1.2 4,281,438 - -0.3 4,281,443 + +0.3 4,281,469 + -0.5 4,281,532 + IAI46_20290 0.10 +1.1 4,281,532 + IAI46_20290 0.10 -0.5 4,281,532 + IAI46_20290 0.10 +0.7 4,281,532 + IAI46_20290 0.10 -1.6 4,281,533 - IAI46_20290 0.11 +0.1 4,281,533 - IAI46_20290 0.11 +0.5 4,281,533 - IAI46_20290 0.11 -1.0 4,281,712 + IAI46_20290 0.34 -0.5 4,281,721 + IAI46_20290 0.35 +0.9 4,281,722 - IAI46_20290 0.35 -0.5 4,281,722 - IAI46_20290 0.35 +0.5
Or see this region's nucleotide sequence