Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_17550 and IAI46_17555 are separated by 11 nucleotides IAI46_17555 and IAI46_17560 overlap by 1 nucleotides IAI46_17560 and IAI46_17565 are separated by 251 nucleotides
IAI46_17550: IAI46_17550 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 3,690,729 to 3,691,586
_17550
IAI46_17555: IAI46_17555 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 3,691,598 to 3,692,365
_17555
IAI46_17560: IAI46_17560 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 3,692,365 to 3,694,038
_17560
IAI46_17565: IAI46_17565 - isochorismate synthase MenF, at 3,694,290 to 3,695,582
_17565
Position (kb)
3692
3693
3694
3695 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3691.370 kb on + strand, within IAI46_17550 at 3691.371 kb on - strand, within IAI46_17550 at 3691.469 kb on + strand, within IAI46_17550 at 3691.507 kb on + strand at 3691.515 kb on - strand at 3691.559 kb on - strand at 3691.579 kb on + strand at 3691.666 kb on + strand at 3692.041 kb on + strand, within IAI46_17555 at 3692.042 kb on - strand, within IAI46_17555 at 3692.042 kb on - strand, within IAI46_17555 at 3692.093 kb on + strand, within IAI46_17555 at 3692.094 kb on - strand, within IAI46_17555 at 3692.094 kb on - strand, within IAI46_17555 at 3692.094 kb on - strand, within IAI46_17555 at 3692.127 kb on + strand, within IAI46_17555 at 3692.367 kb on - strand at 3692.367 kb on - strand at 3692.571 kb on - strand, within IAI46_17560 at 3692.631 kb on + strand, within IAI46_17560 at 3692.632 kb on - strand, within IAI46_17560 at 3692.697 kb on - strand, within IAI46_17560 at 3693.122 kb on + strand, within IAI46_17560 at 3693.122 kb on + strand, within IAI46_17560 at 3693.244 kb on + strand, within IAI46_17560 at 3693.494 kb on + strand, within IAI46_17560 at 3693.543 kb on + strand, within IAI46_17560 at 3693.543 kb on + strand, within IAI46_17560 at 3693.543 kb on + strand, within IAI46_17560 at 3693.544 kb on - strand, within IAI46_17560 at 3693.544 kb on - strand, within IAI46_17560 at 3693.612 kb on + strand, within IAI46_17560 at 3693.613 kb on - strand, within IAI46_17560 at 3693.889 kb on - strand at 3694.038 kb on + strand at 3694.039 kb on - strand at 3694.039 kb on - strand at 3694.117 kb on + strand at 3694.119 kb on + strand at 3694.119 kb on + strand at 3694.120 kb on - strand at 3694.120 kb on - strand at 3694.125 kb on + strand at 3694.125 kb on + strand at 3694.126 kb on - strand at 3694.135 kb on + strand at 3694.166 kb on - strand at 3694.264 kb on + strand at 3694.324 kb on - strand at 3694.408 kb on - strand at 3694.475 kb on - strand, within IAI46_17565 at 3694.492 kb on + strand, within IAI46_17565 at 3694.493 kb on - strand, within IAI46_17565 at 3694.694 kb on + strand, within IAI46_17565 at 3694.694 kb on + strand, within IAI46_17565 at 3694.695 kb on - strand, within IAI46_17565 at 3694.814 kb on + strand, within IAI46_17565 at 3694.864 kb on - strand, within IAI46_17565 at 3694.864 kb on - strand, within IAI46_17565 at 3694.867 kb on + strand, within IAI46_17565 at 3694.868 kb on - strand, within IAI46_17565 at 3694.922 kb on + strand, within IAI46_17565 at 3694.922 kb on + strand, within IAI46_17565 at 3694.923 kb on - strand, within IAI46_17565 at 3694.923 kb on - strand, within IAI46_17565 at 3694.923 kb on - strand, within IAI46_17565 at 3694.925 kb on - strand, within IAI46_17565 at 3694.925 kb on - strand, within IAI46_17565 at 3694.925 kb on - strand, within IAI46_17565 at 3695.002 kb on - strand, within IAI46_17565
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 3,691,370 + IAI46_17550 0.75 -0.1 3,691,371 - IAI46_17550 0.75 +0.4 3,691,469 + IAI46_17550 0.86 -0.0 3,691,507 + +0.4 3,691,515 - +0.5 3,691,559 - -0.8 3,691,579 + -0.2 3,691,666 + -0.5 3,692,041 + IAI46_17555 0.58 +0.5 3,692,042 - IAI46_17555 0.58 -0.8 3,692,042 - IAI46_17555 0.58 +0.5 3,692,093 + IAI46_17555 0.64 +1.5 3,692,094 - IAI46_17555 0.65 +0.7 3,692,094 - IAI46_17555 0.65 +0.5 3,692,094 - IAI46_17555 0.65 +0.7 3,692,127 + IAI46_17555 0.69 +0.2 3,692,367 - -0.3 3,692,367 - +0.5 3,692,571 - IAI46_17560 0.12 +2.1 3,692,631 + IAI46_17560 0.16 -0.2 3,692,632 - IAI46_17560 0.16 -0.3 3,692,697 - IAI46_17560 0.20 +0.3 3,693,122 + IAI46_17560 0.45 -0.5 3,693,122 + IAI46_17560 0.45 -0.0 3,693,244 + IAI46_17560 0.53 -0.6 3,693,494 + IAI46_17560 0.67 -0.1 3,693,543 + IAI46_17560 0.70 -1.3 3,693,543 + IAI46_17560 0.70 -0.1 3,693,543 + IAI46_17560 0.70 +0.7 3,693,544 - IAI46_17560 0.70 -0.1 3,693,544 - IAI46_17560 0.70 -0.6 3,693,612 + IAI46_17560 0.74 -0.3 3,693,613 - IAI46_17560 0.75 -0.3 3,693,889 - +0.1 3,694,038 + -0.2 3,694,039 - +0.2 3,694,039 - +0.7 3,694,117 + +1.2 3,694,119 + +0.9 3,694,119 + +0.4 3,694,120 - -0.4 3,694,120 - +1.7 3,694,125 + -2.1 3,694,125 + -0.9 3,694,126 - -0.6 3,694,135 + +1.0 3,694,166 - +1.1 3,694,264 + -0.0 3,694,324 - -0.1 3,694,408 - +0.1 3,694,475 - IAI46_17565 0.14 +0.4 3,694,492 + IAI46_17565 0.16 -1.0 3,694,493 - IAI46_17565 0.16 -0.6 3,694,694 + IAI46_17565 0.31 +0.5 3,694,694 + IAI46_17565 0.31 +0.4 3,694,695 - IAI46_17565 0.31 +0.4 3,694,814 + IAI46_17565 0.41 -0.4 3,694,864 - IAI46_17565 0.44 -0.2 3,694,864 - IAI46_17565 0.44 -0.2 3,694,867 + IAI46_17565 0.45 -0.5 3,694,868 - IAI46_17565 0.45 +0.4 3,694,922 + IAI46_17565 0.49 +0.4 3,694,922 + IAI46_17565 0.49 +1.5 3,694,923 - IAI46_17565 0.49 +0.4 3,694,923 - IAI46_17565 0.49 -1.4 3,694,923 - IAI46_17565 0.49 +0.1 3,694,925 - IAI46_17565 0.49 -0.2 3,694,925 - IAI46_17565 0.49 +0.5 3,694,925 - IAI46_17565 0.49 +0.9 3,695,002 - IAI46_17565 0.55 +1.3
Or see this region's nucleotide sequence