Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_16930 and IAI46_16935 are separated by 25 nucleotides IAI46_16935 and IAI46_16940 are separated by 69 nucleotides
IAI46_16930: IAI46_16930 - L-idonate 5-dehydrogenase, at 3,550,243 to 3,551,289
_16930
IAI46_16935: IAI46_16935 - alanyl-tRNA editing protein, at 3,551,315 to 3,551,944
_16935
IAI46_16940: IAI46_16940 - LysR family transcriptional regulator, at 3,552,014 to 3,552,964
_16940
Position (kb)
3551
3552 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3550.344 kb on - strand at 3550.387 kb on + strand, within IAI46_16930 at 3550.387 kb on + strand, within IAI46_16930 at 3550.388 kb on - strand, within IAI46_16930 at 3550.509 kb on - strand, within IAI46_16930 at 3550.533 kb on + strand, within IAI46_16930 at 3550.578 kb on + strand, within IAI46_16930 at 3550.579 kb on - strand, within IAI46_16930 at 3550.590 kb on + strand, within IAI46_16930 at 3550.620 kb on + strand, within IAI46_16930 at 3550.639 kb on + strand, within IAI46_16930 at 3550.639 kb on + strand, within IAI46_16930 at 3550.673 kb on - strand, within IAI46_16930 at 3550.698 kb on + strand, within IAI46_16930 at 3550.730 kb on + strand, within IAI46_16930 at 3550.730 kb on + strand, within IAI46_16930 at 3550.731 kb on - strand, within IAI46_16930 at 3550.731 kb on - strand, within IAI46_16930 at 3550.731 kb on - strand, within IAI46_16930 at 3550.731 kb on - strand, within IAI46_16930 at 3550.775 kb on + strand, within IAI46_16930 at 3550.775 kb on + strand, within IAI46_16930 at 3550.779 kb on + strand, within IAI46_16930 at 3550.779 kb on + strand, within IAI46_16930 at 3550.806 kb on - strand, within IAI46_16930 at 3550.961 kb on - strand, within IAI46_16930 at 3551.158 kb on - strand, within IAI46_16930 at 3551.288 kb on - strand at 3551.288 kb on - strand at 3551.298 kb on + strand at 3551.299 kb on - strand at 3551.424 kb on + strand, within IAI46_16935 at 3551.424 kb on + strand, within IAI46_16935 at 3551.425 kb on - strand, within IAI46_16935 at 3551.447 kb on + strand, within IAI46_16935 at 3551.795 kb on - strand, within IAI46_16935 at 3551.909 kb on + strand at 3551.923 kb on - strand at 3551.925 kb on + strand at 3551.925 kb on + strand at 3551.925 kb on + strand at 3551.926 kb on - strand at 3551.926 kb on - strand at 3551.926 kb on - strand at 3551.929 kb on - strand at 3551.929 kb on - strand at 3551.974 kb on + strand at 3551.975 kb on - strand at 3552.008 kb on - strand at 3552.161 kb on - strand, within IAI46_16940 at 3552.193 kb on - strand, within IAI46_16940 at 3552.309 kb on + strand, within IAI46_16940 at 3552.309 kb on + strand, within IAI46_16940 at 3552.309 kb on + strand, within IAI46_16940 at 3552.310 kb on - strand, within IAI46_16940 at 3552.360 kb on - strand, within IAI46_16940 at 3552.360 kb on - strand, within IAI46_16940 at 3552.379 kb on - strand, within IAI46_16940 at 3552.379 kb on - strand, within IAI46_16940 at 3552.408 kb on + strand, within IAI46_16940 at 3552.409 kb on - strand, within IAI46_16940 at 3552.409 kb on - strand, within IAI46_16940 at 3552.437 kb on + strand, within IAI46_16940 at 3552.437 kb on + strand, within IAI46_16940 at 3552.439 kb on + strand, within IAI46_16940 at 3552.440 kb on - strand, within IAI46_16940 at 3552.558 kb on - strand, within IAI46_16940 at 3552.558 kb on - strand, within IAI46_16940 at 3552.617 kb on + strand, within IAI46_16940 at 3552.617 kb on + strand, within IAI46_16940 at 3552.618 kb on - strand, within IAI46_16940 at 3552.687 kb on + strand, within IAI46_16940 at 3552.687 kb on + strand, within IAI46_16940 at 3552.688 kb on - strand, within IAI46_16940 at 3552.688 kb on - strand, within IAI46_16940 at 3552.768 kb on - strand, within IAI46_16940 at 3552.842 kb on + strand, within IAI46_16940 at 3552.866 kb on + strand, within IAI46_16940 at 3552.867 kb on - strand, within IAI46_16940 at 3552.881 kb on + strand at 3552.882 kb on - strand at 3552.907 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 3,550,344 - -1.5 3,550,387 + IAI46_16930 0.14 +1.5 3,550,387 + IAI46_16930 0.14 +0.4 3,550,388 - IAI46_16930 0.14 +0.2 3,550,509 - IAI46_16930 0.25 +1.3 3,550,533 + IAI46_16930 0.28 +0.4 3,550,578 + IAI46_16930 0.32 +0.2 3,550,579 - IAI46_16930 0.32 +1.6 3,550,590 + IAI46_16930 0.33 -0.1 3,550,620 + IAI46_16930 0.36 -0.5 3,550,639 + IAI46_16930 0.38 -1.0 3,550,639 + IAI46_16930 0.38 +1.4 3,550,673 - IAI46_16930 0.41 -0.1 3,550,698 + IAI46_16930 0.43 -1.0 3,550,730 + IAI46_16930 0.47 +1.3 3,550,730 + IAI46_16930 0.47 -0.1 3,550,731 - IAI46_16930 0.47 +0.9 3,550,731 - IAI46_16930 0.47 +0.5 3,550,731 - IAI46_16930 0.47 +1.0 3,550,731 - IAI46_16930 0.47 +0.5 3,550,775 + IAI46_16930 0.51 +0.5 3,550,775 + IAI46_16930 0.51 +0.8 3,550,779 + IAI46_16930 0.51 +0.6 3,550,779 + IAI46_16930 0.51 -0.0 3,550,806 - IAI46_16930 0.54 -0.0 3,550,961 - IAI46_16930 0.69 +1.0 3,551,158 - IAI46_16930 0.87 +1.1 3,551,288 - +0.5 3,551,288 - +1.0 3,551,298 + -0.4 3,551,299 - +0.3 3,551,424 + IAI46_16935 0.17 -0.7 3,551,424 + IAI46_16935 0.17 -0.2 3,551,425 - IAI46_16935 0.17 +0.9 3,551,447 + IAI46_16935 0.21 +0.1 3,551,795 - IAI46_16935 0.76 +0.2 3,551,909 + +0.3 3,551,923 - -0.3 3,551,925 + -0.7 3,551,925 + +0.2 3,551,925 + +0.3 3,551,926 - +0.5 3,551,926 - -0.4 3,551,926 - +0.4 3,551,929 - -0.5 3,551,929 - +0.0 3,551,974 + -1.5 3,551,975 - +1.0 3,552,008 - -0.2 3,552,161 - IAI46_16940 0.15 +1.4 3,552,193 - IAI46_16940 0.19 -0.1 3,552,309 + IAI46_16940 0.31 +0.6 3,552,309 + IAI46_16940 0.31 +0.4 3,552,309 + IAI46_16940 0.31 +0.2 3,552,310 - IAI46_16940 0.31 -0.7 3,552,360 - IAI46_16940 0.36 +0.4 3,552,360 - IAI46_16940 0.36 -1.1 3,552,379 - IAI46_16940 0.38 +0.6 3,552,379 - IAI46_16940 0.38 -0.7 3,552,408 + IAI46_16940 0.41 -0.7 3,552,409 - IAI46_16940 0.42 -0.1 3,552,409 - IAI46_16940 0.42 -0.3 3,552,437 + IAI46_16940 0.44 +0.3 3,552,437 + IAI46_16940 0.44 -0.2 3,552,439 + IAI46_16940 0.45 +1.0 3,552,440 - IAI46_16940 0.45 +0.9 3,552,558 - IAI46_16940 0.57 -1.3 3,552,558 - IAI46_16940 0.57 +1.0 3,552,617 + IAI46_16940 0.63 -0.3 3,552,617 + IAI46_16940 0.63 +1.4 3,552,618 - IAI46_16940 0.64 +0.8 3,552,687 + IAI46_16940 0.71 +0.0 3,552,687 + IAI46_16940 0.71 +0.6 3,552,688 - IAI46_16940 0.71 -0.2 3,552,688 - IAI46_16940 0.71 -3.0 3,552,768 - IAI46_16940 0.79 -1.4 3,552,842 + IAI46_16940 0.87 +0.6 3,552,866 + IAI46_16940 0.90 +1.5 3,552,867 - IAI46_16940 0.90 -1.2 3,552,881 + +0.1 3,552,882 - -0.1 3,552,907 + -0.1
Or see this region's nucleotide sequence