Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_16375 and IAI46_16380 are separated by 3 nucleotides IAI46_16380 and IAI46_16385 are separated by 2 nucleotides IAI46_16385 and IAI46_16390 overlap by 1 nucleotides IAI46_16390 and IAI46_16395 overlap by 4 nucleotides
IAI46_16375: IAI46_16375 - 2,3-dehydroadipyl-CoA hydratase, at 3,430,948 to 3,431,721
_16375
IAI46_16380: IAI46_16380 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 3,431,725 to 3,432,516
_16380
IAI46_16385: IAI46_16385 - 3-hydroxyacyl-CoA dehydrogenase, at 3,432,519 to 3,434,039
_16385
IAI46_16390: IAI46_16390 - hydroxyphenylacetyl-CoA thioesterase PaaI, at 3,434,039 to 3,434,479
_16390
IAI46_16395: IAI46_16395 - 3-oxoadipyl-CoA thiolase, at 3,434,476 to 3,435,681
_16395
Position (kb)
3432
3433
3434
3435 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3431.707 kb on + strand at 3431.802 kb on + strand at 3431.802 kb on + strand at 3431.870 kb on + strand, within IAI46_16380 at 3431.870 kb on + strand, within IAI46_16380 at 3431.978 kb on + strand, within IAI46_16380 at 3431.990 kb on - strand, within IAI46_16380 at 3432.346 kb on + strand, within IAI46_16380 at 3432.346 kb on + strand, within IAI46_16380 at 3432.791 kb on - strand, within IAI46_16385 at 3433.229 kb on + strand, within IAI46_16385 at 3433.252 kb on - strand, within IAI46_16385 at 3433.448 kb on + strand, within IAI46_16385 at 3433.448 kb on + strand, within IAI46_16385 at 3433.449 kb on - strand, within IAI46_16385 at 3433.449 kb on - strand, within IAI46_16385 at 3433.519 kb on + strand, within IAI46_16385 at 3433.520 kb on - strand, within IAI46_16385 at 3433.520 kb on - strand, within IAI46_16385 at 3433.721 kb on - strand, within IAI46_16385 at 3433.884 kb on - strand, within IAI46_16385 at 3433.888 kb on - strand at 3433.921 kb on + strand at 3433.921 kb on + strand at 3433.968 kb on + strand at 3434.010 kb on + strand at 3434.011 kb on - strand at 3434.050 kb on + strand at 3434.050 kb on + strand at 3434.298 kb on + strand, within IAI46_16390 at 3434.298 kb on + strand, within IAI46_16390 at 3434.299 kb on - strand, within IAI46_16390 at 3434.381 kb on + strand, within IAI46_16390 at 3434.896 kb on + strand, within IAI46_16395
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 3,431,707 + -0.2 3,431,802 + +0.4 3,431,802 + -0.6 3,431,870 + IAI46_16380 0.18 -0.1 3,431,870 + IAI46_16380 0.18 +0.3 3,431,978 + IAI46_16380 0.32 +0.0 3,431,990 - IAI46_16380 0.33 -0.3 3,432,346 + IAI46_16380 0.78 -2.9 3,432,346 + IAI46_16380 0.78 -0.2 3,432,791 - IAI46_16385 0.18 -1.5 3,433,229 + IAI46_16385 0.47 +1.4 3,433,252 - IAI46_16385 0.48 -0.2 3,433,448 + IAI46_16385 0.61 +0.3 3,433,448 + IAI46_16385 0.61 +0.5 3,433,449 - IAI46_16385 0.61 -0.4 3,433,449 - IAI46_16385 0.61 -0.3 3,433,519 + IAI46_16385 0.66 +0.5 3,433,520 - IAI46_16385 0.66 -0.1 3,433,520 - IAI46_16385 0.66 +0.1 3,433,721 - IAI46_16385 0.79 +0.2 3,433,884 - IAI46_16385 0.90 -0.4 3,433,888 - -1.6 3,433,921 + -0.1 3,433,921 + +0.2 3,433,968 + +0.4 3,434,010 + -0.8 3,434,011 - +0.8 3,434,050 + -0.3 3,434,050 + +0.2 3,434,298 + IAI46_16390 0.59 +0.8 3,434,298 + IAI46_16390 0.59 +0.1 3,434,299 - IAI46_16390 0.59 +1.7 3,434,381 + IAI46_16390 0.78 -0.5 3,434,896 + IAI46_16395 0.35 +0.7
Or see this region's nucleotide sequence