Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_16230 and IAI46_16235 are separated by 63 nucleotides IAI46_16235 and IAI46_16240 are separated by 2 nucleotides IAI46_16240 and IAI46_16245 are separated by 100 nucleotides
IAI46_16230: IAI46_16230 - glycoside hydrolase, at 3,401,651 to 3,403,834
_16230
IAI46_16235: IAI46_16235 - CoA transferase, at 3,403,898 to 3,405,283
_16235
IAI46_16240: IAI46_16240 - LysR family transcriptional regulator, at 3,405,286 to 3,406,158
_16240
IAI46_16245: IAI46_16245 - aspartate aminotransferase family protein, at 3,406,259 to 3,407,674
_16245
Position (kb)
3403
3404
3405
3406 Strain fitness (log2 ratio)
-2
-1
0
1 at 3402.977 kb on - strand, within IAI46_16230 at 3403.002 kb on - strand, within IAI46_16230 at 3403.002 kb on - strand, within IAI46_16230 at 3403.002 kb on - strand, within IAI46_16230 at 3403.032 kb on - strand, within IAI46_16230 at 3403.198 kb on + strand, within IAI46_16230 at 3403.318 kb on + strand, within IAI46_16230 at 3403.318 kb on + strand, within IAI46_16230 at 3403.319 kb on - strand, within IAI46_16230 at 3403.319 kb on - strand, within IAI46_16230 at 3403.332 kb on - strand, within IAI46_16230 at 3403.334 kb on + strand, within IAI46_16230 at 3403.335 kb on - strand, within IAI46_16230 at 3403.335 kb on - strand, within IAI46_16230 at 3403.335 kb on - strand, within IAI46_16230 at 3403.445 kb on + strand, within IAI46_16230 at 3403.493 kb on + strand, within IAI46_16230 at 3403.494 kb on - strand, within IAI46_16230 at 3403.550 kb on + strand, within IAI46_16230 at 3403.550 kb on + strand, within IAI46_16230 at 3403.555 kb on + strand, within IAI46_16230 at 3403.832 kb on + strand at 3403.833 kb on - strand at 3403.855 kb on + strand at 3403.855 kb on + strand at 3403.856 kb on - strand at 3403.856 kb on - strand at 3403.860 kb on + strand at 3403.861 kb on - strand at 3403.861 kb on - strand at 3403.910 kb on - strand at 3403.910 kb on - strand at 3404.148 kb on + strand, within IAI46_16235 at 3404.158 kb on - strand, within IAI46_16235 at 3404.233 kb on + strand, within IAI46_16235 at 3404.254 kb on + strand, within IAI46_16235 at 3404.254 kb on + strand, within IAI46_16235 at 3404.589 kb on + strand, within IAI46_16235 at 3404.728 kb on + strand, within IAI46_16235 at 3405.038 kb on + strand, within IAI46_16235 at 3405.223 kb on - strand at 3405.313 kb on + strand at 3405.314 kb on - strand at 3405.314 kb on - strand at 3405.314 kb on - strand at 3405.531 kb on - strand, within IAI46_16240 at 3405.574 kb on - strand, within IAI46_16240 at 3405.574 kb on - strand, within IAI46_16240 at 3405.606 kb on + strand, within IAI46_16240 at 3405.789 kb on + strand, within IAI46_16240 at 3405.789 kb on + strand, within IAI46_16240 at 3405.790 kb on - strand, within IAI46_16240 at 3405.790 kb on - strand, within IAI46_16240 at 3405.790 kb on - strand, within IAI46_16240 at 3405.833 kb on - strand, within IAI46_16240 at 3406.115 kb on - strand at 3406.232 kb on + strand at 3406.232 kb on + strand at 3406.242 kb on + strand at 3406.255 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 3,402,977 - IAI46_16230 0.61 +0.8 3,403,002 - IAI46_16230 0.62 -0.8 3,403,002 - IAI46_16230 0.62 -0.8 3,403,002 - IAI46_16230 0.62 -0.6 3,403,032 - IAI46_16230 0.63 -0.1 3,403,198 + IAI46_16230 0.71 +0.6 3,403,318 + IAI46_16230 0.76 -0.5 3,403,318 + IAI46_16230 0.76 +0.8 3,403,319 - IAI46_16230 0.76 +0.1 3,403,319 - IAI46_16230 0.76 -0.1 3,403,332 - IAI46_16230 0.77 +0.9 3,403,334 + IAI46_16230 0.77 -0.1 3,403,335 - IAI46_16230 0.77 +0.2 3,403,335 - IAI46_16230 0.77 -0.8 3,403,335 - IAI46_16230 0.77 -0.4 3,403,445 + IAI46_16230 0.82 +1.3 3,403,493 + IAI46_16230 0.84 -0.1 3,403,494 - IAI46_16230 0.84 +0.3 3,403,550 + IAI46_16230 0.87 +0.1 3,403,550 + IAI46_16230 0.87 -0.0 3,403,555 + IAI46_16230 0.87 +0.1 3,403,832 + -0.8 3,403,833 - -0.6 3,403,855 + +0.5 3,403,855 + +0.4 3,403,856 - +0.5 3,403,856 - +0.1 3,403,860 + +0.8 3,403,861 - +0.1 3,403,861 - -0.4 3,403,910 - -0.1 3,403,910 - +0.5 3,404,148 + IAI46_16235 0.18 +0.6 3,404,158 - IAI46_16235 0.19 +0.5 3,404,233 + IAI46_16235 0.24 +0.0 3,404,254 + IAI46_16235 0.26 +0.1 3,404,254 + IAI46_16235 0.26 -1.1 3,404,589 + IAI46_16235 0.50 -0.7 3,404,728 + IAI46_16235 0.60 +0.3 3,405,038 + IAI46_16235 0.82 -0.3 3,405,223 - +0.1 3,405,313 + +1.0 3,405,314 - +0.2 3,405,314 - +1.1 3,405,314 - +0.4 3,405,531 - IAI46_16240 0.28 +0.1 3,405,574 - IAI46_16240 0.33 +0.2 3,405,574 - IAI46_16240 0.33 -0.5 3,405,606 + IAI46_16240 0.37 +0.3 3,405,789 + IAI46_16240 0.58 +1.5 3,405,789 + IAI46_16240 0.58 +0.7 3,405,790 - IAI46_16240 0.58 -0.4 3,405,790 - IAI46_16240 0.58 +0.5 3,405,790 - IAI46_16240 0.58 -0.8 3,405,833 - IAI46_16240 0.63 -0.5 3,406,115 - -2.0 3,406,232 + +0.6 3,406,232 + -0.5 3,406,242 + +0.7 3,406,255 - +1.1
Or see this region's nucleotide sequence