Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_15085 and IAI46_15090 are separated by 512 nucleotides IAI46_15090 and IAI46_15095 are separated by 8 nucleotides IAI46_15095 and IAI46_15100 are separated by 13 nucleotides IAI46_15100 and IAI46_15105 overlap by 8 nucleotides
IAI46_15085: IAI46_15085 - hypothetical protein, at 3,179,312 to 3,179,680
_15085
IAI46_15090: IAI46_15090 - hypothetical protein, at 3,180,193 to 3,180,591
_15090
IAI46_15095: IAI46_15095 - lysozyme, at 3,180,600 to 3,181,040
_15095
IAI46_15100: IAI46_15100 - hypothetical protein, at 3,181,054 to 3,181,374
_15100
IAI46_15105: IAI46_15105 - phage holin, lambda family, at 3,181,367 to 3,181,702
_15105
Position (kb)
3180
3181
3182 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3179.612 kb on + strand, within IAI46_15085 at 3179.632 kb on - strand, within IAI46_15085 at 3179.646 kb on - strand at 3179.663 kb on + strand at 3179.707 kb on + strand at 3179.723 kb on - strand at 3179.753 kb on + strand at 3179.754 kb on - strand at 3179.760 kb on - strand at 3179.760 kb on - strand at 3179.760 kb on - strand at 3179.763 kb on + strand at 3179.764 kb on - strand at 3179.764 kb on - strand at 3179.790 kb on + strand at 3179.798 kb on - strand at 3179.817 kb on - strand at 3179.953 kb on - strand at 3179.988 kb on - strand at 3180.040 kb on + strand at 3180.110 kb on + strand at 3180.123 kb on + strand at 3180.143 kb on + strand at 3180.144 kb on - strand at 3180.194 kb on + strand at 3180.238 kb on + strand, within IAI46_15090 at 3180.239 kb on - strand, within IAI46_15090 at 3180.243 kb on + strand, within IAI46_15090 at 3180.244 kb on - strand, within IAI46_15090 at 3180.326 kb on + strand, within IAI46_15090 at 3180.419 kb on - strand, within IAI46_15090 at 3180.419 kb on - strand, within IAI46_15090 at 3180.527 kb on - strand, within IAI46_15090 at 3180.527 kb on - strand, within IAI46_15090 at 3180.575 kb on + strand at 3180.576 kb on - strand at 3180.580 kb on - strand at 3180.585 kb on + strand at 3180.617 kb on + strand at 3180.617 kb on + strand at 3180.618 kb on - strand at 3180.769 kb on + strand, within IAI46_15095 at 3180.770 kb on - strand, within IAI46_15095 at 3180.784 kb on - strand, within IAI46_15095 at 3180.823 kb on - strand, within IAI46_15095 at 3180.845 kb on - strand, within IAI46_15095 at 3180.864 kb on - strand, within IAI46_15095 at 3180.917 kb on + strand, within IAI46_15095 at 3180.944 kb on - strand, within IAI46_15095 at 3180.958 kb on - strand, within IAI46_15095 at 3180.977 kb on - strand, within IAI46_15095 at 3181.032 kb on + strand at 3181.033 kb on - strand at 3181.047 kb on + strand at 3181.052 kb on - strand at 3181.119 kb on + strand, within IAI46_15100 at 3181.120 kb on - strand, within IAI46_15100 at 3181.196 kb on - strand, within IAI46_15100 at 3181.196 kb on - strand, within IAI46_15100 at 3181.232 kb on + strand, within IAI46_15100 at 3181.232 kb on + strand, within IAI46_15100 at 3181.253 kb on + strand, within IAI46_15100 at 3181.328 kb on + strand, within IAI46_15100 at 3181.329 kb on - strand, within IAI46_15100 at 3181.358 kb on - strand at 3181.368 kb on + strand at 3181.369 kb on - strand at 3181.422 kb on + strand, within IAI46_15105 at 3181.490 kb on + strand, within IAI46_15105 at 3181.491 kb on - strand, within IAI46_15105 at 3181.497 kb on - strand, within IAI46_15105 at 3181.497 kb on - strand, within IAI46_15105 at 3181.599 kb on + strand, within IAI46_15105 at 3181.604 kb on + strand, within IAI46_15105 at 3181.605 kb on - strand, within IAI46_15105 at 3181.717 kb on + strand at 3181.728 kb on - strand at 3181.728 kb on - strand at 3181.739 kb on + strand at 3181.747 kb on + strand at 3181.847 kb on + strand at 3181.847 kb on + strand at 3181.847 kb on + strand at 3181.848 kb on - strand at 3181.848 kb on - strand at 3181.854 kb on - strand at 3181.854 kb on - strand at 3181.879 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 3,179,612 + IAI46_15085 0.81 +0.1 3,179,632 - IAI46_15085 0.87 -0.7 3,179,646 - +0.4 3,179,663 + +1.0 3,179,707 + +1.0 3,179,723 - +0.4 3,179,753 + -0.1 3,179,754 - -0.4 3,179,760 - -0.4 3,179,760 - +0.1 3,179,760 - +0.9 3,179,763 + +0.1 3,179,764 - -2.2 3,179,764 - +0.5 3,179,790 + +1.4 3,179,798 - -0.4 3,179,817 - -0.0 3,179,953 - -0.4 3,179,988 - -0.1 3,180,040 + -0.3 3,180,110 + +0.1 3,180,123 + +1.9 3,180,143 + +0.4 3,180,144 - -0.0 3,180,194 + -0.5 3,180,238 + IAI46_15090 0.11 +0.2 3,180,239 - IAI46_15090 0.12 +1.1 3,180,243 + IAI46_15090 0.13 +0.2 3,180,244 - IAI46_15090 0.13 -0.2 3,180,326 + IAI46_15090 0.33 -0.1 3,180,419 - IAI46_15090 0.57 -0.2 3,180,419 - IAI46_15090 0.57 -1.1 3,180,527 - IAI46_15090 0.84 -0.8 3,180,527 - IAI46_15090 0.84 +1.2 3,180,575 + -1.2 3,180,576 - +1.0 3,180,580 - +0.6 3,180,585 + -0.8 3,180,617 + +0.5 3,180,617 + -1.1 3,180,618 - -0.6 3,180,769 + IAI46_15095 0.38 +0.9 3,180,770 - IAI46_15095 0.39 -0.4 3,180,784 - IAI46_15095 0.42 +0.4 3,180,823 - IAI46_15095 0.51 -0.9 3,180,845 - IAI46_15095 0.56 +0.6 3,180,864 - IAI46_15095 0.60 -0.0 3,180,917 + IAI46_15095 0.72 +1.7 3,180,944 - IAI46_15095 0.78 +0.1 3,180,958 - IAI46_15095 0.81 +0.8 3,180,977 - IAI46_15095 0.85 +0.4 3,181,032 + +1.4 3,181,033 - +1.4 3,181,047 + +0.6 3,181,052 - +0.5 3,181,119 + IAI46_15100 0.20 +0.6 3,181,120 - IAI46_15100 0.21 -0.1 3,181,196 - IAI46_15100 0.44 +1.8 3,181,196 - IAI46_15100 0.44 +1.6 3,181,232 + IAI46_15100 0.55 +1.6 3,181,232 + IAI46_15100 0.55 +0.4 3,181,253 + IAI46_15100 0.62 +1.9 3,181,328 + IAI46_15100 0.85 -0.9 3,181,329 - IAI46_15100 0.86 +0.7 3,181,358 - +0.9 3,181,368 + +1.1 3,181,369 - +0.1 3,181,422 + IAI46_15105 0.16 -0.3 3,181,490 + IAI46_15105 0.37 +0.3 3,181,491 - IAI46_15105 0.37 +0.9 3,181,497 - IAI46_15105 0.39 +0.0 3,181,497 - IAI46_15105 0.39 -0.5 3,181,599 + IAI46_15105 0.69 -2.2 3,181,604 + IAI46_15105 0.71 -0.7 3,181,605 - IAI46_15105 0.71 +0.1 3,181,717 + +0.7 3,181,728 - -0.7 3,181,728 - +0.2 3,181,739 + +0.1 3,181,747 + +1.4 3,181,847 + -1.4 3,181,847 + +0.5 3,181,847 + +0.6 3,181,848 - +0.4 3,181,848 - +0.5 3,181,854 - -1.2 3,181,854 - +0.3 3,181,879 + -0.4
Or see this region's nucleotide sequence