Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_10440 and IAI46_10445 are separated by 95 nucleotides IAI46_10445 and IAI46_10450 are separated by 9 nucleotides IAI46_10450 and IAI46_10455 are separated by 21 nucleotides IAI46_10455 and IAI46_10460 are separated by 132 nucleotides
IAI46_10440: IAI46_10440 - tRNA 2-thiouridine(34) synthase MnmA, at 2,207,584 to 2,208,687
_10440
IAI46_10445: IAI46_10445 - NUDIX hydrolase, at 2,208,783 to 2,209,229
_10445
IAI46_10450: IAI46_10450 - hypothetical protein, at 2,209,239 to 2,209,574
_10450
IAI46_10455: IAI46_10455 - 23S rRNA pseudouridine(2457) synthase RluE, at 2,209,596 to 2,210,222
_10455
IAI46_10460: IAI46_10460 - NADP-dependent isocitrate dehydrogenase, at 2,210,355 to 2,211,608
_10460
Position (kb)
2209
2210
2211 Strain fitness (log2 ratio)
-1
0
1
2 at 2208.775 kb on + strand at 2208.776 kb on - strand at 2208.797 kb on + strand at 2208.797 kb on + strand at 2208.798 kb on - strand at 2208.798 kb on - strand at 2209.001 kb on + strand, within IAI46_10445 at 2209.154 kb on + strand, within IAI46_10445 at 2209.155 kb on - strand, within IAI46_10445 at 2209.224 kb on - strand at 2209.229 kb on + strand at 2209.230 kb on - strand at 2209.256 kb on + strand at 2209.256 kb on + strand at 2209.257 kb on - strand at 2209.264 kb on + strand at 2209.264 kb on + strand at 2209.284 kb on - strand, within IAI46_10450 at 2209.408 kb on - strand, within IAI46_10450 at 2209.417 kb on + strand, within IAI46_10450 at 2209.450 kb on - strand, within IAI46_10450 at 2209.454 kb on + strand, within IAI46_10450 at 2209.455 kb on - strand, within IAI46_10450 at 2209.564 kb on - strand at 2209.598 kb on - strand at 2209.598 kb on - strand at 2209.658 kb on + strand at 2209.658 kb on + strand at 2209.659 kb on - strand, within IAI46_10455 at 2209.659 kb on - strand, within IAI46_10455 at 2209.663 kb on + strand, within IAI46_10455 at 2209.682 kb on + strand, within IAI46_10455 at 2209.925 kb on - strand, within IAI46_10455 at 2210.253 kb on + strand at 2210.297 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 2,208,775 + -0.4 2,208,776 - +0.3 2,208,797 + +0.8 2,208,797 + +0.2 2,208,798 - -0.3 2,208,798 - +0.6 2,209,001 + IAI46_10445 0.49 +1.0 2,209,154 + IAI46_10445 0.83 +0.4 2,209,155 - IAI46_10445 0.83 +0.9 2,209,224 - +1.9 2,209,229 + +0.1 2,209,230 - -0.6 2,209,256 + -0.8 2,209,256 + +1.3 2,209,257 - -0.9 2,209,264 + -0.4 2,209,264 + +0.1 2,209,284 - IAI46_10450 0.13 +0.6 2,209,408 - IAI46_10450 0.50 -0.3 2,209,417 + IAI46_10450 0.53 +1.7 2,209,450 - IAI46_10450 0.63 +0.2 2,209,454 + IAI46_10450 0.64 -0.3 2,209,455 - IAI46_10450 0.64 -0.5 2,209,564 - +0.0 2,209,598 - +1.0 2,209,598 - +0.5 2,209,658 + +0.6 2,209,658 + +0.4 2,209,659 - IAI46_10455 0.10 +0.2 2,209,659 - IAI46_10455 0.10 +0.2 2,209,663 + IAI46_10455 0.11 +0.8 2,209,682 + IAI46_10455 0.14 -0.4 2,209,925 - IAI46_10455 0.52 -0.6 2,210,253 + +0.7 2,210,297 + +0.4
Or see this region's nucleotide sequence