Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_08270 and IAI46_08275 are separated by 69 nucleotides IAI46_08275 and IAI46_08280 are separated by 14 nucleotides
IAI46_08270: IAI46_08270 - isopenicillin N synthase family oxygenase, at 1,759,469 to 1,760,488
_08270
IAI46_08275: IAI46_08275 - PLP-dependent transferase, at 1,760,558 to 1,761,703
_08275
IAI46_08280: IAI46_08280 - cystathionine beta-synthase, at 1,761,718 to 1,763,085
_08280
Position (kb)
1760
1761
1762 Strain fitness (log2 ratio)
-1
0
1
2 at 1759.618 kb on + strand, within IAI46_08270 at 1759.724 kb on + strand, within IAI46_08270 at 1759.726 kb on + strand, within IAI46_08270 at 1759.726 kb on + strand, within IAI46_08270 at 1759.726 kb on + strand, within IAI46_08270 at 1759.893 kb on + strand, within IAI46_08270 at 1760.014 kb on + strand, within IAI46_08270 at 1760.015 kb on - strand, within IAI46_08270 at 1760.179 kb on + strand, within IAI46_08270 at 1760.409 kb on + strand at 1760.409 kb on + strand at 1760.416 kb on + strand at 1760.417 kb on - strand at 1760.466 kb on - strand at 1760.466 kb on - strand at 1760.468 kb on + strand at 1760.641 kb on + strand at 1760.644 kb on + strand at 1760.645 kb on - strand at 1760.675 kb on - strand, within IAI46_08275 at 1760.744 kb on - strand, within IAI46_08275 at 1760.779 kb on - strand, within IAI46_08275 at 1760.878 kb on - strand, within IAI46_08275 at 1760.878 kb on - strand, within IAI46_08275 at 1760.878 kb on - strand, within IAI46_08275 at 1760.880 kb on + strand, within IAI46_08275 at 1760.923 kb on + strand, within IAI46_08275 at 1760.923 kb on + strand, within IAI46_08275 at 1760.924 kb on - strand, within IAI46_08275 at 1760.954 kb on - strand, within IAI46_08275 at 1761.064 kb on + strand, within IAI46_08275 at 1761.114 kb on + strand, within IAI46_08275 at 1761.134 kb on + strand, within IAI46_08275 at 1761.134 kb on + strand, within IAI46_08275 at 1761.169 kb on + strand, within IAI46_08275 at 1761.170 kb on - strand, within IAI46_08275 at 1761.170 kb on - strand, within IAI46_08275 at 1761.171 kb on + strand, within IAI46_08275 at 1761.172 kb on - strand, within IAI46_08275 at 1761.172 kb on - strand, within IAI46_08275 at 1761.172 kb on - strand, within IAI46_08275 at 1761.172 kb on - strand, within IAI46_08275 at 1761.172 kb on - strand, within IAI46_08275 at 1761.348 kb on + strand, within IAI46_08275 at 1761.349 kb on - strand, within IAI46_08275 at 1761.349 kb on - strand, within IAI46_08275 at 1761.400 kb on - strand, within IAI46_08275 at 1761.400 kb on - strand, within IAI46_08275 at 1761.498 kb on + strand, within IAI46_08275 at 1761.573 kb on + strand, within IAI46_08275 at 1761.574 kb on - strand, within IAI46_08275 at 1761.613 kb on - strand at 1761.666 kb on + strand at 1761.689 kb on - strand at 1761.703 kb on + strand at 1761.716 kb on + strand at 1761.717 kb on - strand at 1761.717 kb on - strand at 1761.830 kb on - strand at 1761.840 kb on - strand at 1762.002 kb on - strand, within IAI46_08280 at 1762.021 kb on + strand, within IAI46_08280 at 1762.021 kb on + strand, within IAI46_08280 at 1762.021 kb on + strand, within IAI46_08280 at 1762.021 kb on + strand, within IAI46_08280 at 1762.022 kb on - strand, within IAI46_08280 at 1762.022 kb on - strand, within IAI46_08280 at 1762.022 kb on - strand, within IAI46_08280 at 1762.022 kb on - strand, within IAI46_08280 at 1762.022 kb on - strand, within IAI46_08280 at 1762.022 kb on - strand, within IAI46_08280 at 1762.152 kb on + strand, within IAI46_08280 at 1762.154 kb on + strand, within IAI46_08280 at 1762.154 kb on + strand, within IAI46_08280 at 1762.155 kb on - strand, within IAI46_08280 at 1762.169 kb on + strand, within IAI46_08280 at 1762.169 kb on + strand, within IAI46_08280 at 1762.170 kb on - strand, within IAI46_08280 at 1762.335 kb on + strand, within IAI46_08280 at 1762.340 kb on + strand, within IAI46_08280 at 1762.360 kb on - strand, within IAI46_08280 at 1762.360 kb on - strand, within IAI46_08280 at 1762.445 kb on + strand, within IAI46_08280 at 1762.445 kb on + strand, within IAI46_08280 at 1762.446 kb on - strand, within IAI46_08280 at 1762.657 kb on - strand, within IAI46_08280 at 1762.659 kb on - strand, within IAI46_08280
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 1,759,618 + IAI46_08270 0.15 +0.4 1,759,724 + IAI46_08270 0.25 +0.4 1,759,726 + IAI46_08270 0.25 +0.9 1,759,726 + IAI46_08270 0.25 -0.4 1,759,726 + IAI46_08270 0.25 -0.6 1,759,893 + IAI46_08270 0.42 -0.1 1,760,014 + IAI46_08270 0.53 +0.7 1,760,015 - IAI46_08270 0.54 -0.8 1,760,179 + IAI46_08270 0.70 +0.3 1,760,409 + -0.4 1,760,409 + -0.4 1,760,416 + +0.4 1,760,417 - -0.2 1,760,466 - -0.9 1,760,466 - +0.6 1,760,468 + +0.1 1,760,641 + +0.3 1,760,644 + +0.5 1,760,645 - -0.3 1,760,675 - IAI46_08275 0.10 +0.4 1,760,744 - IAI46_08275 0.16 +0.2 1,760,779 - IAI46_08275 0.19 -0.5 1,760,878 - IAI46_08275 0.28 -0.6 1,760,878 - IAI46_08275 0.28 +0.0 1,760,878 - IAI46_08275 0.28 -0.1 1,760,880 + IAI46_08275 0.28 -0.3 1,760,923 + IAI46_08275 0.32 +0.2 1,760,923 + IAI46_08275 0.32 +0.1 1,760,924 - IAI46_08275 0.32 +0.4 1,760,954 - IAI46_08275 0.35 -0.7 1,761,064 + IAI46_08275 0.44 -0.0 1,761,114 + IAI46_08275 0.49 +2.0 1,761,134 + IAI46_08275 0.50 -0.0 1,761,134 + IAI46_08275 0.50 +0.8 1,761,169 + IAI46_08275 0.53 +0.9 1,761,170 - IAI46_08275 0.53 +0.2 1,761,170 - IAI46_08275 0.53 -1.3 1,761,171 + IAI46_08275 0.53 +0.5 1,761,172 - IAI46_08275 0.54 +0.3 1,761,172 - IAI46_08275 0.54 +0.4 1,761,172 - IAI46_08275 0.54 -0.2 1,761,172 - IAI46_08275 0.54 +0.8 1,761,172 - IAI46_08275 0.54 -0.1 1,761,348 + IAI46_08275 0.69 +0.1 1,761,349 - IAI46_08275 0.69 -0.9 1,761,349 - IAI46_08275 0.69 -0.6 1,761,400 - IAI46_08275 0.73 -0.6 1,761,400 - IAI46_08275 0.73 +0.3 1,761,498 + IAI46_08275 0.82 -0.2 1,761,573 + IAI46_08275 0.89 +0.2 1,761,574 - IAI46_08275 0.89 -0.2 1,761,613 - +1.3 1,761,666 + -0.0 1,761,689 - +0.1 1,761,703 + +0.8 1,761,716 + +0.6 1,761,717 - +0.1 1,761,717 - -1.6 1,761,830 - -0.2 1,761,840 - -0.3 1,762,002 - IAI46_08280 0.21 +0.7 1,762,021 + IAI46_08280 0.22 -0.3 1,762,021 + IAI46_08280 0.22 -0.2 1,762,021 + IAI46_08280 0.22 +0.1 1,762,021 + IAI46_08280 0.22 -1.6 1,762,022 - IAI46_08280 0.22 +0.3 1,762,022 - IAI46_08280 0.22 +0.0 1,762,022 - IAI46_08280 0.22 +1.7 1,762,022 - IAI46_08280 0.22 +0.2 1,762,022 - IAI46_08280 0.22 +0.3 1,762,022 - IAI46_08280 0.22 +1.0 1,762,152 + IAI46_08280 0.32 -0.5 1,762,154 + IAI46_08280 0.32 +1.7 1,762,154 + IAI46_08280 0.32 -1.5 1,762,155 - IAI46_08280 0.32 +0.5 1,762,169 + IAI46_08280 0.33 -0.0 1,762,169 + IAI46_08280 0.33 -0.7 1,762,170 - IAI46_08280 0.33 -0.4 1,762,335 + IAI46_08280 0.45 +0.1 1,762,340 + IAI46_08280 0.45 +0.6 1,762,360 - IAI46_08280 0.47 -0.3 1,762,360 - IAI46_08280 0.47 -0.4 1,762,445 + IAI46_08280 0.53 +0.4 1,762,445 + IAI46_08280 0.53 -1.3 1,762,446 - IAI46_08280 0.53 +0.5 1,762,657 - IAI46_08280 0.69 -0.2 1,762,659 - IAI46_08280 0.69 -0.8
Or see this region's nucleotide sequence