Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_07520 and IAI46_07525 are separated by 160 nucleotides IAI46_07525 and IAI46_07530 overlap by 4 nucleotides IAI46_07530 and IAI46_07535 are separated by 105 nucleotides
IAI46_07520: IAI46_07520 - mechanosensitive channel protein, at 1,589,487 to 1,591,706
_07520
IAI46_07525: IAI46_07525 - glutamine ABC transporter ATP-binding protein GlnQ, at 1,591,867 to 1,592,589
_07525
IAI46_07530: IAI46_07530 - glutamine ABC transporter permease GlnP, at 1,592,586 to 1,593,245
_07530
IAI46_07535: IAI46_07535 - glutamine ABC transporter substrate-binding protein GlnH, at 1,593,351 to 1,594,094
_07535
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1591.627 kb on + strand at 1591.708 kb on + strand at 1591.708 kb on + strand at 1591.708 kb on + strand at 1591.708 kb on + strand at 1591.709 kb on - strand at 1591.709 kb on - strand at 1591.709 kb on - strand at 1591.728 kb on + strand at 1591.770 kb on + strand at 1591.770 kb on + strand at 1591.770 kb on + strand at 1591.771 kb on - strand at 1591.812 kb on + strand at 1591.822 kb on + strand at 1591.822 kb on + strand at 1591.823 kb on - strand at 1591.823 kb on - strand at 1591.863 kb on + strand at 1592.344 kb on + strand, within IAI46_07525 at 1592.345 kb on - strand, within IAI46_07525 at 1592.345 kb on - strand, within IAI46_07525 at 1592.345 kb on - strand, within IAI46_07525 at 1592.518 kb on - strand at 1592.645 kb on + strand at 1592.653 kb on + strand, within IAI46_07530 at 1592.768 kb on + strand, within IAI46_07530 at 1592.829 kb on - strand, within IAI46_07530 at 1592.907 kb on + strand, within IAI46_07530 at 1592.908 kb on - strand, within IAI46_07530 at 1593.023 kb on - strand, within IAI46_07530 at 1593.026 kb on + strand, within IAI46_07530 at 1593.027 kb on - strand, within IAI46_07530 at 1593.039 kb on + strand, within IAI46_07530 at 1593.039 kb on + strand, within IAI46_07530 at 1593.040 kb on - strand, within IAI46_07530 at 1593.076 kb on - strand, within IAI46_07530 at 1593.110 kb on - strand, within IAI46_07530 at 1593.110 kb on - strand, within IAI46_07530 at 1593.114 kb on - strand, within IAI46_07530 at 1593.199 kb on - strand at 1593.284 kb on + strand at 1593.285 kb on - strand at 1593.323 kb on + strand at 1593.323 kb on + strand at 1593.324 kb on - strand at 1593.357 kb on - strand at 1593.394 kb on + strand at 1593.395 kb on - strand at 1593.395 kb on - strand at 1593.395 kb on - strand at 1593.545 kb on - strand, within IAI46_07535 at 1593.603 kb on - strand, within IAI46_07535 at 1593.604 kb on + strand, within IAI46_07535 at 1593.605 kb on - strand, within IAI46_07535 at 1593.617 kb on + strand, within IAI46_07535 at 1593.891 kb on - strand, within IAI46_07535 at 1593.904 kb on + strand, within IAI46_07535 at 1593.905 kb on - strand, within IAI46_07535 at 1593.905 kb on - strand, within IAI46_07535 at 1593.905 kb on - strand, within IAI46_07535 at 1593.961 kb on + strand, within IAI46_07535 at 1593.961 kb on + strand, within IAI46_07535 at 1593.962 kb on - strand, within IAI46_07535 at 1593.965 kb on + strand, within IAI46_07535 at 1594.110 kb on + strand at 1594.111 kb on - strand at 1594.111 kb on - strand at 1594.185 kb on + strand at 1594.230 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 1,591,627 + +0.2 1,591,708 + +0.5 1,591,708 + +1.3 1,591,708 + -0.3 1,591,708 + +0.3 1,591,709 - +0.4 1,591,709 - +0.4 1,591,709 - +0.0 1,591,728 + -0.4 1,591,770 + -1.1 1,591,770 + -0.0 1,591,770 + +0.9 1,591,771 - -0.1 1,591,812 + -0.3 1,591,822 + +0.0 1,591,822 + +0.4 1,591,823 - +0.3 1,591,823 - +0.4 1,591,863 + +0.2 1,592,344 + IAI46_07525 0.66 +0.1 1,592,345 - IAI46_07525 0.66 +0.2 1,592,345 - IAI46_07525 0.66 +0.2 1,592,345 - IAI46_07525 0.66 +0.4 1,592,518 - +0.4 1,592,645 + -1.1 1,592,653 + IAI46_07530 0.10 +0.5 1,592,768 + IAI46_07530 0.28 +0.4 1,592,829 - IAI46_07530 0.37 -0.0 1,592,907 + IAI46_07530 0.49 +1.1 1,592,908 - IAI46_07530 0.49 -0.5 1,593,023 - IAI46_07530 0.66 -0.4 1,593,026 + IAI46_07530 0.67 -0.0 1,593,027 - IAI46_07530 0.67 -0.7 1,593,039 + IAI46_07530 0.69 +1.9 1,593,039 + IAI46_07530 0.69 +0.1 1,593,040 - IAI46_07530 0.69 -0.2 1,593,076 - IAI46_07530 0.74 +0.4 1,593,110 - IAI46_07530 0.79 +0.3 1,593,110 - IAI46_07530 0.79 -0.4 1,593,114 - IAI46_07530 0.80 +0.6 1,593,199 - +0.7 1,593,284 + -0.4 1,593,285 - -0.1 1,593,323 + +0.6 1,593,323 + +0.7 1,593,324 - +0.7 1,593,357 - +0.3 1,593,394 + +1.6 1,593,395 - +0.1 1,593,395 - -1.5 1,593,395 - -0.1 1,593,545 - IAI46_07535 0.26 +0.5 1,593,603 - IAI46_07535 0.34 +0.4 1,593,604 + IAI46_07535 0.34 -0.0 1,593,605 - IAI46_07535 0.34 +0.0 1,593,617 + IAI46_07535 0.36 +1.0 1,593,891 - IAI46_07535 0.73 +0.0 1,593,904 + IAI46_07535 0.74 -0.2 1,593,905 - IAI46_07535 0.74 +0.6 1,593,905 - IAI46_07535 0.74 +0.2 1,593,905 - IAI46_07535 0.74 -2.7 1,593,961 + IAI46_07535 0.82 +0.0 1,593,961 + IAI46_07535 0.82 +0.2 1,593,962 - IAI46_07535 0.82 -0.2 1,593,965 + IAI46_07535 0.83 -1.0 1,594,110 + +0.3 1,594,111 - +0.6 1,594,111 - +0.4 1,594,185 + +0.6 1,594,230 + +0.9
Or see this region's nucleotide sequence