Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_06215 and IAI46_06220 overlap by 7 nucleotides IAI46_06220 and IAI46_06225 overlap by 11 nucleotides IAI46_06225 and IAI46_06230 are separated by 170 nucleotides IAI46_06230 and IAI46_06235 overlap by 4 nucleotides
IAI46_06215: IAI46_06215 - 5-oxoprolinase subunit PxpB, at 1,330,883 to 1,331,539
_06215
IAI46_06220: IAI46_06220 - biotin-dependent carboxyltransferase family protein, at 1,331,533 to 1,332,465
_06220
IAI46_06225: IAI46_06225 - 5-oxoprolinase subunit PxpA, at 1,332,455 to 1,333,192
_06225
IAI46_06230: IAI46_06230 - DUF969 domain-containing protein, at 1,333,363 to 1,334,094
_06230
IAI46_06235: IAI46_06235 - DUF979 domain-containing protein, at 1,334,091 to 1,335,110
_06235
Position (kb)
1332
1333
1334 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1331.465 kb on + strand, within IAI46_06215 at 1331.532 kb on + strand at 1331.532 kb on + strand at 1331.532 kb on + strand at 1331.532 kb on + strand at 1331.560 kb on + strand at 1331.673 kb on + strand, within IAI46_06220 at 1331.866 kb on + strand, within IAI46_06220 at 1331.866 kb on + strand, within IAI46_06220 at 1331.866 kb on + strand, within IAI46_06220 at 1331.958 kb on + strand, within IAI46_06220 at 1331.959 kb on - strand, within IAI46_06220 at 1332.249 kb on + strand, within IAI46_06220 at 1332.261 kb on + strand, within IAI46_06220 at 1332.288 kb on + strand, within IAI46_06220 at 1332.288 kb on + strand, within IAI46_06220 at 1332.334 kb on + strand, within IAI46_06220 at 1332.335 kb on - strand, within IAI46_06220 at 1332.603 kb on - strand, within IAI46_06225 at 1332.689 kb on + strand, within IAI46_06225 at 1332.689 kb on + strand, within IAI46_06225 at 1332.690 kb on - strand, within IAI46_06225 at 1332.690 kb on - strand, within IAI46_06225 at 1332.690 kb on - strand, within IAI46_06225 at 1332.704 kb on + strand, within IAI46_06225 at 1332.705 kb on - strand, within IAI46_06225 at 1332.783 kb on - strand, within IAI46_06225 at 1332.783 kb on - strand, within IAI46_06225 at 1333.039 kb on - strand, within IAI46_06225 at 1333.200 kb on + strand at 1333.206 kb on + strand at 1333.206 kb on + strand at 1333.206 kb on + strand at 1333.284 kb on + strand at 1333.284 kb on + strand at 1333.284 kb on + strand at 1333.285 kb on - strand at 1333.285 kb on - strand at 1333.292 kb on + strand at 1333.293 kb on - strand at 1333.320 kb on - strand at 1333.320 kb on - strand at 1333.377 kb on + strand at 1333.378 kb on - strand at 1333.378 kb on - strand at 1333.383 kb on - strand at 1333.383 kb on - strand at 1333.383 kb on - strand at 1333.435 kb on - strand at 1333.468 kb on - strand, within IAI46_06230 at 1333.537 kb on - strand, within IAI46_06230 at 1333.537 kb on - strand, within IAI46_06230 at 1333.672 kb on + strand, within IAI46_06230 at 1333.846 kb on - strand, within IAI46_06230 at 1333.902 kb on + strand, within IAI46_06230 at 1333.915 kb on - strand, within IAI46_06230 at 1333.932 kb on + strand, within IAI46_06230 at 1333.980 kb on + strand, within IAI46_06230 at 1333.980 kb on + strand, within IAI46_06230 at 1333.980 kb on + strand, within IAI46_06230 at 1334.014 kb on + strand, within IAI46_06230 at 1334.025 kb on + strand at 1334.056 kb on - strand at 1334.109 kb on + strand at 1334.109 kb on + strand at 1334.110 kb on - strand at 1334.110 kb on - strand at 1334.110 kb on - strand at 1334.116 kb on - strand at 1334.186 kb on + strand at 1334.187 kb on - strand at 1334.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 1,331,465 + IAI46_06215 0.89 -2.8 1,331,532 + +1.1 1,331,532 + +0.6 1,331,532 + -0.2 1,331,532 + +0.3 1,331,560 + +0.6 1,331,673 + IAI46_06220 0.15 +0.2 1,331,866 + IAI46_06220 0.36 -0.3 1,331,866 + IAI46_06220 0.36 +0.2 1,331,866 + IAI46_06220 0.36 +0.2 1,331,958 + IAI46_06220 0.46 +0.1 1,331,959 - IAI46_06220 0.46 +1.1 1,332,249 + IAI46_06220 0.77 -0.2 1,332,261 + IAI46_06220 0.78 +0.2 1,332,288 + IAI46_06220 0.81 -1.1 1,332,288 + IAI46_06220 0.81 -0.9 1,332,334 + IAI46_06220 0.86 -0.5 1,332,335 - IAI46_06220 0.86 -0.3 1,332,603 - IAI46_06225 0.20 +0.2 1,332,689 + IAI46_06225 0.32 +1.4 1,332,689 + IAI46_06225 0.32 +0.7 1,332,690 - IAI46_06225 0.32 +0.7 1,332,690 - IAI46_06225 0.32 +0.1 1,332,690 - IAI46_06225 0.32 -0.1 1,332,704 + IAI46_06225 0.34 -2.4 1,332,705 - IAI46_06225 0.34 +0.6 1,332,783 - IAI46_06225 0.44 -0.4 1,332,783 - IAI46_06225 0.44 +0.3 1,333,039 - IAI46_06225 0.79 -0.4 1,333,200 + -0.1 1,333,206 + +1.2 1,333,206 + +0.4 1,333,206 + -0.5 1,333,284 + +0.1 1,333,284 + +0.9 1,333,284 + +0.6 1,333,285 - -1.5 1,333,285 - -0.1 1,333,292 + +1.4 1,333,293 - -0.5 1,333,320 - +0.1 1,333,320 - +0.2 1,333,377 + +0.6 1,333,378 - +0.2 1,333,378 - +0.3 1,333,383 - -1.1 1,333,383 - +0.1 1,333,383 - +0.3 1,333,435 - -0.2 1,333,468 - IAI46_06230 0.14 -1.3 1,333,537 - IAI46_06230 0.24 +0.5 1,333,537 - IAI46_06230 0.24 +0.7 1,333,672 + IAI46_06230 0.42 +0.7 1,333,846 - IAI46_06230 0.66 +0.1 1,333,902 + IAI46_06230 0.74 +0.2 1,333,915 - IAI46_06230 0.75 -0.1 1,333,932 + IAI46_06230 0.78 +0.5 1,333,980 + IAI46_06230 0.84 -0.5 1,333,980 + IAI46_06230 0.84 -0.9 1,333,980 + IAI46_06230 0.84 +0.3 1,334,014 + IAI46_06230 0.89 -0.8 1,334,025 + +0.3 1,334,056 - -0.1 1,334,109 + +0.5 1,334,109 + -0.3 1,334,110 - -0.3 1,334,110 - +0.9 1,334,110 - +0.0 1,334,116 - +0.4 1,334,186 + +0.3 1,334,187 - +0.5 1,334,190 - +0.7
Or see this region's nucleotide sequence