Experiment: 30_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS21930 and AMB_RS21935 overlap by 4 nucleotides AMB_RS21935 and AMB_RS21940 overlap by 4 nucleotides AMB_RS21940 and AMB_RS21945 overlap by 4 nucleotides AMB_RS21945 and AMB_RS21950 are separated by 64 nucleotides
AMB_RS21930: AMB_RS21930 - cobalt transporter CbiM, at 4,741,111 to 4,741,710
_RS21930
AMB_RS21935: AMB_RS21935 - hypothetical protein, at 4,741,707 to 4,742,228
_RS21935
AMB_RS21940: AMB_RS21940 - cobalt ECF transporter T component CbiQ, at 4,742,225 to 4,742,956
_RS21940
AMB_RS21945: AMB_RS21945 - ABC transporter ATP-binding protein, at 4,742,953 to 4,743,651
_RS21945
AMB_RS21950: AMB_RS21950 - hypothetical protein, at 4,743,716 to 4,744,912
_RS21950
Position (kb)
4742
4743 Strain fitness (log2 ratio)
-2
-1
0
1 at 4743.653 kb on + strand at 4743.653 kb on + strand at 4743.653 kb on + strand at 4743.653 kb on + strand at 4743.654 kb on - strand at 4743.654 kb on - strand at 4743.654 kb on - strand at 4743.654 kb on - strand at 4743.654 kb on - strand at 4743.714 kb on + strand at 4743.825 kb on + strand at 4743.825 kb on + strand at 4743.825 kb on + strand at 4743.825 kb on + strand at 4743.825 kb on + strand at 4743.826 kb on - strand at 4743.850 kb on - strand, within AMB_RS21950
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_1 remove 4,743,653 + +0.1 4,743,653 + -0.2 4,743,653 + +0.8 4,743,653 + -1.0 4,743,654 - -0.8 4,743,654 - -1.0 4,743,654 - -0.3 4,743,654 - -0.5 4,743,654 - -0.5 4,743,714 + -2.6 4,743,825 + -0.6 4,743,825 + +0.4 4,743,825 + +0.5 4,743,825 + -0.9 4,743,825 + +0.2 4,743,826 - +1.0 4,743,850 - AMB_RS21950 0.11 +0.2
Or see this region's nucleotide sequence