Experiment: 30_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS16220 and AMB_RS16225 overlap by 4 nucleotides AMB_RS16225 and AMB_RS16230 are separated by 9 nucleotides AMB_RS16230 and AMB_RS16235 overlap by 1 nucleotides AMB_RS16235 and AMB_RS16240 are separated by 5 nucleotides
AMB_RS16220: AMB_RS16220 - protein TolB, at 3,478,597 to 3,479,940
_RS16220
AMB_RS16225: AMB_RS16225 - energy transducer TonB, at 3,479,937 to 3,480,878
_RS16225
AMB_RS16230: AMB_RS16230 - protein TolR, at 3,480,888 to 3,481,349
_RS16230
AMB_RS16235: AMB_RS16235 - protein TolQ, at 3,481,349 to 3,482,074
_RS16235
AMB_RS16240: AMB_RS16240 - tol-pal system-associated acyl-CoA thioesterase, at 3,482,080 to 3,482,661
_RS16240
Position (kb)
3480
3481
3482 Strain fitness (log2 ratio)
-2
-1
0
1 at 3482.082 kb on - strand at 3482.161 kb on - strand, within AMB_RS16240 at 3482.161 kb on - strand, within AMB_RS16240 at 3482.163 kb on - strand, within AMB_RS16240 at 3482.163 kb on - strand, within AMB_RS16240 at 3482.163 kb on - strand, within AMB_RS16240 at 3482.163 kb on - strand, within AMB_RS16240 at 3482.234 kb on - strand, within AMB_RS16240 at 3482.234 kb on - strand, within AMB_RS16240 at 3482.234 kb on - strand, within AMB_RS16240 at 3482.234 kb on - strand, within AMB_RS16240 at 3482.234 kb on - strand, within AMB_RS16240 at 3482.236 kb on - strand, within AMB_RS16240
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_1 remove 3,482,082 - -0.6 3,482,161 - AMB_RS16240 0.14 -1.2 3,482,161 - AMB_RS16240 0.14 -0.4 3,482,163 - AMB_RS16240 0.14 -2.0 3,482,163 - AMB_RS16240 0.14 -0.2 3,482,163 - AMB_RS16240 0.14 -0.0 3,482,163 - AMB_RS16240 0.14 -0.3 3,482,234 - AMB_RS16240 0.26 -0.6 3,482,234 - AMB_RS16240 0.26 -0.6 3,482,234 - AMB_RS16240 0.26 -0.7 3,482,234 - AMB_RS16240 0.26 -0.0 3,482,234 - AMB_RS16240 0.26 -0.1 3,482,236 - AMB_RS16240 0.27 -1.3
Or see this region's nucleotide sequence