Experiment: 30_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08435 and AMB_RS08440 are separated by 142 nucleotides AMB_RS08440 and AMB_RS08445 are separated by 132 nucleotides
AMB_RS08435: AMB_RS08435 - radical SAM protein, at 1,804,868 to 1,805,743
_RS08435
AMB_RS08440: AMB_RS08440 - amidase, at 1,805,886 to 1,807,061
_RS08440
AMB_RS08445: AMB_RS08445 - signal transduction protein, at 1,807,194 to 1,808,876
_RS08445
Position (kb)
1805
1806
1807
1808 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1804.949 kb on + strand at 1804.950 kb on - strand at 1804.976 kb on + strand, within AMB_RS08435 at 1804.976 kb on + strand, within AMB_RS08435 at 1804.976 kb on + strand, within AMB_RS08435 at 1804.976 kb on + strand, within AMB_RS08435 at 1804.977 kb on - strand, within AMB_RS08435 at 1804.977 kb on - strand, within AMB_RS08435 at 1804.991 kb on + strand, within AMB_RS08435 at 1805.090 kb on + strand, within AMB_RS08435 at 1805.090 kb on + strand, within AMB_RS08435 at 1805.091 kb on - strand, within AMB_RS08435 at 1805.091 kb on - strand, within AMB_RS08435 at 1805.091 kb on - strand, within AMB_RS08435 at 1805.171 kb on + strand, within AMB_RS08435 at 1805.172 kb on - strand, within AMB_RS08435 at 1805.249 kb on + strand, within AMB_RS08435 at 1805.249 kb on + strand, within AMB_RS08435 at 1805.249 kb on + strand, within AMB_RS08435 at 1805.249 kb on + strand, within AMB_RS08435 at 1805.250 kb on - strand, within AMB_RS08435 at 1805.250 kb on - strand, within AMB_RS08435 at 1805.453 kb on + strand, within AMB_RS08435 at 1805.453 kb on + strand, within AMB_RS08435 at 1805.453 kb on + strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.454 kb on - strand, within AMB_RS08435 at 1805.741 kb on + strand at 1805.742 kb on - strand at 1805.742 kb on - strand at 1805.742 kb on - strand at 1805.749 kb on + strand at 1805.750 kb on - strand at 1805.750 kb on - strand at 1805.798 kb on + strand at 1805.798 kb on + strand at 1805.798 kb on + strand at 1805.799 kb on - strand at 1805.799 kb on - strand at 1805.799 kb on - strand at 1805.799 kb on - strand at 1805.799 kb on - strand at 1805.800 kb on + strand at 1805.800 kb on + strand at 1805.800 kb on + strand at 1805.800 kb on + strand at 1805.801 kb on - strand at 1806.944 kb on - strand at 1806.944 kb on - strand at 1806.944 kb on - strand at 1807.019 kb on - strand at 1807.019 kb on - strand at 1807.019 kb on - strand at 1807.075 kb on + strand at 1807.075 kb on + strand at 1807.076 kb on - strand at 1807.076 kb on - strand at 1807.076 kb on - strand at 1807.112 kb on - strand at 1807.195 kb on + strand at 1807.237 kb on + strand at 1807.237 kb on + strand at 1807.237 kb on + strand at 1807.237 kb on + strand at 1807.237 kb on + strand at 1807.238 kb on - strand at 1807.240 kb on + strand at 1807.241 kb on - strand at 1807.241 kb on - strand at 1807.241 kb on - strand at 1807.241 kb on - strand at 1807.241 kb on - strand at 1807.463 kb on - strand, within AMB_RS08445 at 1807.547 kb on + strand, within AMB_RS08445 at 1807.585 kb on + strand, within AMB_RS08445 at 1807.585 kb on + strand, within AMB_RS08445 at 1807.586 kb on - strand, within AMB_RS08445 at 1807.586 kb on - strand, within AMB_RS08445 at 1807.669 kb on + strand, within AMB_RS08445 at 1807.669 kb on + strand, within AMB_RS08445 at 1807.669 kb on + strand, within AMB_RS08445 at 1807.669 kb on + strand, within AMB_RS08445 at 1807.669 kb on + strand, within AMB_RS08445 at 1807.669 kb on + strand, within AMB_RS08445 at 1807.669 kb on + strand, within AMB_RS08445 at 1807.669 kb on + strand, within AMB_RS08445 at 1807.669 kb on + strand, within AMB_RS08445 at 1807.670 kb on - strand, within AMB_RS08445 at 1807.670 kb on - strand, within AMB_RS08445 at 1807.670 kb on - strand, within AMB_RS08445 at 1807.670 kb on - strand, within AMB_RS08445 at 1807.879 kb on + strand, within AMB_RS08445
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_1 remove 1,804,949 + -1.2 1,804,950 - +0.8 1,804,976 + AMB_RS08435 0.12 +0.1 1,804,976 + AMB_RS08435 0.12 -0.3 1,804,976 + AMB_RS08435 0.12 +1.2 1,804,976 + AMB_RS08435 0.12 -0.3 1,804,977 - AMB_RS08435 0.12 +0.4 1,804,977 - AMB_RS08435 0.12 -0.3 1,804,991 + AMB_RS08435 0.14 -0.3 1,805,090 + AMB_RS08435 0.25 +0.1 1,805,090 + AMB_RS08435 0.25 -0.5 1,805,091 - AMB_RS08435 0.25 -0.8 1,805,091 - AMB_RS08435 0.25 -0.7 1,805,091 - AMB_RS08435 0.25 -0.6 1,805,171 + AMB_RS08435 0.35 -0.3 1,805,172 - AMB_RS08435 0.35 +0.9 1,805,249 + AMB_RS08435 0.43 +1.2 1,805,249 + AMB_RS08435 0.43 -0.0 1,805,249 + AMB_RS08435 0.43 +0.4 1,805,249 + AMB_RS08435 0.43 +0.5 1,805,250 - AMB_RS08435 0.44 +0.7 1,805,250 - AMB_RS08435 0.44 +1.5 1,805,453 + AMB_RS08435 0.67 +1.0 1,805,453 + AMB_RS08435 0.67 +0.1 1,805,453 + AMB_RS08435 0.67 -0.5 1,805,454 - AMB_RS08435 0.67 +0.1 1,805,454 - AMB_RS08435 0.67 -0.1 1,805,454 - AMB_RS08435 0.67 -0.4 1,805,454 - AMB_RS08435 0.67 +1.0 1,805,454 - AMB_RS08435 0.67 -0.5 1,805,454 - AMB_RS08435 0.67 -0.5 1,805,454 - AMB_RS08435 0.67 -0.4 1,805,454 - AMB_RS08435 0.67 -0.7 1,805,454 - AMB_RS08435 0.67 +0.3 1,805,454 - AMB_RS08435 0.67 +0.4 1,805,741 + +0.1 1,805,742 - +0.5 1,805,742 - -0.4 1,805,742 - -1.2 1,805,749 + +0.6 1,805,750 - -0.6 1,805,750 - +0.1 1,805,798 + +0.6 1,805,798 + +0.3 1,805,798 + +0.1 1,805,799 - +0.2 1,805,799 - +0.1 1,805,799 - +0.1 1,805,799 - -2.6 1,805,799 - -1.0 1,805,800 + -1.5 1,805,800 + +0.4 1,805,800 + -0.5 1,805,800 + -0.8 1,805,801 - -0.1 1,806,944 - +0.0 1,806,944 - +0.0 1,806,944 - +0.8 1,807,019 - +1.2 1,807,019 - -0.0 1,807,019 - +1.0 1,807,075 + +0.0 1,807,075 + -0.1 1,807,076 - -0.6 1,807,076 - +0.6 1,807,076 - +1.0 1,807,112 - -0.2 1,807,195 + -0.5 1,807,237 + +0.1 1,807,237 + -0.7 1,807,237 + -0.4 1,807,237 + +1.2 1,807,237 + +1.7 1,807,238 - +0.0 1,807,240 + +0.5 1,807,241 - -0.2 1,807,241 - +0.4 1,807,241 - -0.4 1,807,241 - +1.4 1,807,241 - -0.0 1,807,463 - AMB_RS08445 0.16 -1.1 1,807,547 + AMB_RS08445 0.21 -0.2 1,807,585 + AMB_RS08445 0.23 -1.9 1,807,585 + AMB_RS08445 0.23 +0.9 1,807,586 - AMB_RS08445 0.23 -0.4 1,807,586 - AMB_RS08445 0.23 -0.1 1,807,669 + AMB_RS08445 0.28 +2.4 1,807,669 + AMB_RS08445 0.28 -0.2 1,807,669 + AMB_RS08445 0.28 -1.5 1,807,669 + AMB_RS08445 0.28 -0.9 1,807,669 + AMB_RS08445 0.28 +0.2 1,807,669 + AMB_RS08445 0.28 -0.5 1,807,669 + AMB_RS08445 0.28 -1.1 1,807,669 + AMB_RS08445 0.28 -0.2 1,807,669 + AMB_RS08445 0.28 -1.6 1,807,670 - AMB_RS08445 0.28 -1.8 1,807,670 - AMB_RS08445 0.28 +0.2 1,807,670 - AMB_RS08445 0.28 -0.4 1,807,670 - AMB_RS08445 0.28 -0.8 1,807,879 + AMB_RS08445 0.41 -0.3
Or see this region's nucleotide sequence