Experiment: 30_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08350 and AMB_RS08355 are separated by 393 nucleotides AMB_RS08355 and AMB_RS08360 overlap by 32 nucleotides AMB_RS08360 and AMB_RS08365 overlap by 4 nucleotides
AMB_RS08350: AMB_RS08350 - hydrogenase 2 small subunit, at 1,787,615 to 1,788,745
_RS08350
AMB_RS08355: AMB_RS08355 - hypothetical protein, at 1,789,139 to 1,789,423
_RS08355
AMB_RS08360: AMB_RS08360 - hypothetical protein, at 1,789,392 to 1,789,937
_RS08360
AMB_RS08365: AMB_RS08365 - CoA transferase, at 1,789,934 to 1,791,145
_RS08365
Position (kb)
1789
1790 Strain fitness (log2 ratio)
-2
-1
0
1 at 1788.396 kb on + strand, within AMB_RS08350 at 1788.396 kb on + strand, within AMB_RS08350 at 1788.396 kb on + strand, within AMB_RS08350 at 1788.396 kb on + strand, within AMB_RS08350 at 1788.396 kb on + strand, within AMB_RS08350 at 1788.397 kb on - strand, within AMB_RS08350 at 1788.397 kb on - strand, within AMB_RS08350 at 1788.397 kb on - strand, within AMB_RS08350 at 1788.397 kb on - strand, within AMB_RS08350 at 1788.397 kb on - strand, within AMB_RS08350 at 1788.477 kb on + strand, within AMB_RS08350 at 1788.556 kb on + strand, within AMB_RS08350 at 1788.556 kb on + strand, within AMB_RS08350 at 1788.741 kb on + strand at 1788.741 kb on + strand at 1788.741 kb on + strand at 1788.741 kb on + strand at 1788.742 kb on - strand at 1788.742 kb on - strand at 1788.938 kb on - strand at 1789.104 kb on - strand at 1789.201 kb on - strand, within AMB_RS08355 at 1789.218 kb on + strand, within AMB_RS08355 at 1789.218 kb on + strand, within AMB_RS08355 at 1789.218 kb on + strand, within AMB_RS08355 at 1789.218 kb on + strand, within AMB_RS08355 at 1789.218 kb on + strand, within AMB_RS08355 at 1789.219 kb on - strand, within AMB_RS08355 at 1789.219 kb on - strand, within AMB_RS08355 at 1789.293 kb on - strand, within AMB_RS08355 at 1789.393 kb on + strand at 1789.394 kb on - strand at 1789.422 kb on - strand at 1789.465 kb on + strand, within AMB_RS08360 at 1789.661 kb on + strand, within AMB_RS08360 at 1789.661 kb on + strand, within AMB_RS08360 at 1789.662 kb on - strand, within AMB_RS08360 at 1789.871 kb on + strand, within AMB_RS08360
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_1 remove 1,788,396 + AMB_RS08350 0.69 +0.6 1,788,396 + AMB_RS08350 0.69 -0.6 1,788,396 + AMB_RS08350 0.69 -1.7 1,788,396 + AMB_RS08350 0.69 -0.1 1,788,396 + AMB_RS08350 0.69 -0.7 1,788,397 - AMB_RS08350 0.69 -0.3 1,788,397 - AMB_RS08350 0.69 -1.8 1,788,397 - AMB_RS08350 0.69 +0.5 1,788,397 - AMB_RS08350 0.69 -1.0 1,788,397 - AMB_RS08350 0.69 -1.4 1,788,477 + AMB_RS08350 0.76 -2.6 1,788,556 + AMB_RS08350 0.83 -0.3 1,788,556 + AMB_RS08350 0.83 -1.0 1,788,741 + +0.1 1,788,741 + +0.4 1,788,741 + -1.9 1,788,741 + +0.4 1,788,742 - -0.5 1,788,742 - +1.2 1,788,938 - -1.4 1,789,104 - -0.6 1,789,201 - AMB_RS08355 0.22 -0.1 1,789,218 + AMB_RS08355 0.28 +0.2 1,789,218 + AMB_RS08355 0.28 -0.2 1,789,218 + AMB_RS08355 0.28 +0.4 1,789,218 + AMB_RS08355 0.28 -0.2 1,789,218 + AMB_RS08355 0.28 +1.6 1,789,219 - AMB_RS08355 0.28 -0.4 1,789,219 - AMB_RS08355 0.28 +0.4 1,789,293 - AMB_RS08355 0.54 -0.6 1,789,393 + +1.4 1,789,394 - +0.1 1,789,422 - +0.4 1,789,465 + AMB_RS08360 0.13 -0.1 1,789,661 + AMB_RS08360 0.49 +1.1 1,789,661 + AMB_RS08360 0.49 -0.9 1,789,662 - AMB_RS08360 0.49 -0.1 1,789,871 + AMB_RS08360 0.88 -1.0
Or see this region's nucleotide sequence