Experiment: 30_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05890 and AMB_RS05895 overlap by 14 nucleotides AMB_RS05895 and AMB_RS05900 are separated by 257 nucleotides AMB_RS05900 and AMB_RS05905 are separated by 86 nucleotides AMB_RS05905 and AMB_RS05910 are separated by 3 nucleotides AMB_RS05910 and AMB_RS05915 overlap by 31 nucleotides AMB_RS05915 and AMB_RS05920 are separated by 11 nucleotides
AMB_RS05890: AMB_RS05890 - DNA methylase, at 1,242,081 to 1,243,349
_RS05890
AMB_RS05895: AMB_RS05895 - hypothetical protein, at 1,243,336 to 1,243,545
_RS05895
AMB_RS05900: AMB_RS05900 - hypothetical protein, at 1,243,803 to 1,244,015
_RS05900
AMB_RS05905: AMB_RS05905 - DNA-binding protein, at 1,244,102 to 1,244,356
_RS05905
AMB_RS05910: AMB_RS05910 - hypothetical protein, at 1,244,360 to 1,244,557
_RS05910
AMB_RS05915: AMB_RS05915 - hypothetical protein, at 1,244,527 to 1,244,724
_RS05915
AMB_RS05920: AMB_RS05920 - DUF3489 domain-containing protein, at 1,244,736 to 1,245,323
_RS05920
Position (kb) 1243
1244
1245 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1242.825 kb on + strand, within AMB_RS05890 at 1242.825 kb on + strand, within AMB_RS05890 at 1242.825 kb on + strand, within AMB_RS05890 at 1242.825 kb on + strand, within AMB_RS05890 at 1242.825 kb on + strand, within AMB_RS05890 at 1242.825 kb on + strand, within AMB_RS05890 at 1242.826 kb on - strand, within AMB_RS05890 at 1242.826 kb on - strand, within AMB_RS05890 at 1242.846 kb on + strand, within AMB_RS05890 at 1242.846 kb on + strand, within AMB_RS05890 at 1242.846 kb on + strand, within AMB_RS05890 at 1242.847 kb on - strand, within AMB_RS05890 at 1242.945 kb on + strand, within AMB_RS05890 at 1242.946 kb on - strand, within AMB_RS05890 at 1242.946 kb on - strand, within AMB_RS05890 at 1242.946 kb on - strand, within AMB_RS05890 at 1242.972 kb on + strand, within AMB_RS05890 at 1242.972 kb on + strand, within AMB_RS05890 at 1242.973 kb on - strand, within AMB_RS05890 at 1242.973 kb on - strand, within AMB_RS05890 at 1242.973 kb on - strand, within AMB_RS05890 at 1243.233 kb on + strand at 1243.268 kb on - strand at 1243.268 kb on - strand at 1243.268 kb on - strand at 1243.268 kb on - strand at 1243.371 kb on - strand, within AMB_RS05895 at 1243.371 kb on - strand, within AMB_RS05895 at 1243.457 kb on + strand, within AMB_RS05895 at 1243.458 kb on - strand, within AMB_RS05895 at 1243.476 kb on - strand, within AMB_RS05895 at 1243.476 kb on - strand, within AMB_RS05895 at 1243.545 kb on + strand at 1243.546 kb on - strand at 1243.546 kb on - strand at 1243.548 kb on + strand at 1243.548 kb on + strand at 1243.548 kb on + strand at 1243.549 kb on - strand at 1243.549 kb on - strand at 1243.549 kb on - strand at 1243.549 kb on - strand at 1243.572 kb on + strand at 1243.573 kb on - strand at 1243.573 kb on - strand at 1243.977 kb on + strand, within AMB_RS05900 at 1243.978 kb on - strand, within AMB_RS05900 at 1243.978 kb on - strand, within AMB_RS05900 at 1244.049 kb on + strand at 1244.049 kb on + strand at 1244.050 kb on - strand at 1244.092 kb on + strand at 1244.093 kb on - strand at 1244.093 kb on - strand at 1244.354 kb on + strand at 1244.354 kb on + strand at 1244.354 kb on + strand at 1244.354 kb on + strand at 1244.354 kb on + strand at 1244.355 kb on - strand at 1244.355 kb on - strand at 1244.355 kb on - strand at 1244.415 kb on - strand, within AMB_RS05910 at 1244.415 kb on - strand, within AMB_RS05910 at 1244.415 kb on - strand, within AMB_RS05910 at 1244.555 kb on + strand at 1244.714 kb on + strand at 1244.715 kb on - strand at 1244.715 kb on - strand at 1244.715 kb on - strand at 1244.752 kb on + strand at 1244.756 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_1 remove 1,242,825 + AMB_RS05890 0.59 +2.9 1,242,825 + AMB_RS05890 0.59 +0.1 1,242,825 + AMB_RS05890 0.59 -0.5 1,242,825 + AMB_RS05890 0.59 +0.1 1,242,825 + AMB_RS05890 0.59 -0.8 1,242,825 + AMB_RS05890 0.59 +0.4 1,242,826 - AMB_RS05890 0.59 -0.1 1,242,826 - AMB_RS05890 0.59 +0.4 1,242,846 + AMB_RS05890 0.60 -1.1 1,242,846 + AMB_RS05890 0.60 -0.2 1,242,846 + AMB_RS05890 0.60 +0.3 1,242,847 - AMB_RS05890 0.60 +0.6 1,242,945 + AMB_RS05890 0.68 +0.2 1,242,946 - AMB_RS05890 0.68 -0.1 1,242,946 - AMB_RS05890 0.68 +1.9 1,242,946 - AMB_RS05890 0.68 -0.5 1,242,972 + AMB_RS05890 0.70 -1.1 1,242,972 + AMB_RS05890 0.70 -0.6 1,242,973 - AMB_RS05890 0.70 +0.4 1,242,973 - AMB_RS05890 0.70 -0.1 1,242,973 - AMB_RS05890 0.70 -1.1 1,243,233 + +0.8 1,243,268 - -0.0 1,243,268 - -0.6 1,243,268 - -0.0 1,243,268 - -1.0 1,243,371 - AMB_RS05895 0.17 +0.6 1,243,371 - AMB_RS05895 0.17 -0.1 1,243,457 + AMB_RS05895 0.58 -2.2 1,243,458 - AMB_RS05895 0.58 -0.4 1,243,476 - AMB_RS05895 0.67 +0.4 1,243,476 - AMB_RS05895 0.67 -0.1 1,243,545 + -0.6 1,243,546 - +0.4 1,243,546 - -0.5 1,243,548 + -0.4 1,243,548 + +0.0 1,243,548 + +0.9 1,243,549 - -0.2 1,243,549 - -1.1 1,243,549 - -0.4 1,243,549 - -1.3 1,243,572 + +0.8 1,243,573 - -0.1 1,243,573 - +1.3 1,243,977 + AMB_RS05900 0.82 -0.7 1,243,978 - AMB_RS05900 0.82 -0.1 1,243,978 - AMB_RS05900 0.82 +0.0 1,244,049 + -0.3 1,244,049 + -0.3 1,244,050 - -0.5 1,244,092 + +1.9 1,244,093 - -0.3 1,244,093 - -0.3 1,244,354 + +0.9 1,244,354 + -0.5 1,244,354 + -0.2 1,244,354 + -0.3 1,244,354 + -0.3 1,244,355 - -0.5 1,244,355 - -1.4 1,244,355 - +0.2 1,244,415 - AMB_RS05910 0.28 -0.4 1,244,415 - AMB_RS05910 0.28 -0.6 1,244,415 - AMB_RS05910 0.28 +0.2 1,244,555 + +0.5 1,244,714 + -0.3 1,244,715 - -0.0 1,244,715 - +1.3 1,244,715 - -0.6 1,244,752 + -0.4 1,244,756 + +0.4
Or see this region's nucleotide sequence