Experiment: 30_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03975 and AMB_RS03980 are separated by 90 nucleotides AMB_RS03980 and AMB_RS03985 are separated by 65 nucleotides AMB_RS03985 and AMB_RS03990 are separated by 1 nucleotides AMB_RS03990 and AMB_RS03995 overlap by 4 nucleotides
AMB_RS03975: AMB_RS03975 - hypothetical protein, at 833,410 to 834,744
_RS03975
AMB_RS03980: AMB_RS03980 - serine/threonine protein phosphatase, at 834,835 to 835,623
_RS03980
AMB_RS03985: AMB_RS03985 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase, at 835,689 to 837,155
_RS03985
AMB_RS03990: AMB_RS03990 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 837,157 to 837,618
_RS03990
AMB_RS03995: AMB_RS03995 - tRNA epoxyqueuosine(34) reductase QueG, at 837,615 to 838,697
_RS03995
Position (kb) 835
836
837
838 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 834.813 kb on - strand at 834.813 kb on - strand at 834.813 kb on - strand at 834.813 kb on - strand at 834.813 kb on - strand at 834.813 kb on - strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.814 kb on + strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.815 kb on - strand at 834.816 kb on + strand at 834.816 kb on + strand at 834.816 kb on + strand at 834.816 kb on + strand at 834.816 kb on + strand at 834.816 kb on + strand at 834.817 kb on - strand at 834.817 kb on - strand at 834.817 kb on - strand at 834.846 kb on + strand at 834.882 kb on - strand at 834.882 kb on - strand at 834.931 kb on + strand, within AMB_RS03980 at 834.931 kb on + strand, within AMB_RS03980 at 834.931 kb on + strand, within AMB_RS03980 at 834.932 kb on - strand, within AMB_RS03980 at 834.932 kb on - strand, within AMB_RS03980 at 835.240 kb on + strand, within AMB_RS03980 at 835.240 kb on + strand, within AMB_RS03980 at 835.240 kb on + strand, within AMB_RS03980 at 835.240 kb on + strand, within AMB_RS03980 at 835.240 kb on + strand, within AMB_RS03980 at 835.240 kb on + strand, within AMB_RS03980 at 835.240 kb on + strand, within AMB_RS03980 at 835.240 kb on + strand, within AMB_RS03980 at 835.241 kb on - strand, within AMB_RS03980 at 835.241 kb on - strand, within AMB_RS03980 at 835.241 kb on - strand, within AMB_RS03980 at 835.241 kb on - strand, within AMB_RS03980 at 835.241 kb on - strand, within AMB_RS03980 at 835.241 kb on - strand, within AMB_RS03980 at 835.241 kb on - strand, within AMB_RS03980 at 835.276 kb on + strand, within AMB_RS03980 at 835.276 kb on + strand, within AMB_RS03980 at 835.369 kb on + strand, within AMB_RS03980 at 835.369 kb on + strand, within AMB_RS03980 at 835.370 kb on - strand, within AMB_RS03980 at 835.370 kb on - strand, within AMB_RS03980 at 835.370 kb on - strand, within AMB_RS03980 at 835.617 kb on + strand at 837.268 kb on + strand, within AMB_RS03990 at 837.268 kb on + strand, within AMB_RS03990 at 837.269 kb on - strand, within AMB_RS03990 at 837.269 kb on - strand, within AMB_RS03990 at 837.269 kb on - strand, within AMB_RS03990 at 837.352 kb on + strand, within AMB_RS03990 at 837.760 kb on - strand, within AMB_RS03995 at 837.876 kb on + strand, within AMB_RS03995 at 837.876 kb on + strand, within AMB_RS03995 at 837.876 kb on + strand, within AMB_RS03995 at 837.877 kb on - strand, within AMB_RS03995 at 837.877 kb on - strand, within AMB_RS03995 at 837.877 kb on - strand, within AMB_RS03995 at 837.877 kb on - strand, within AMB_RS03995 at 837.929 kb on + strand, within AMB_RS03995 at 837.939 kb on + strand, within AMB_RS03995 at 837.940 kb on - strand, within AMB_RS03995 at 837.940 kb on - strand, within AMB_RS03995 at 837.940 kb on - strand, within AMB_RS03995 at 837.957 kb on + strand, within AMB_RS03995 at 838.112 kb on + strand, within AMB_RS03995
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_1 remove 834,813 - -1.2 834,813 - -1.2 834,813 - -0.2 834,813 - +0.1 834,813 - -0.4 834,813 - +0.3 834,814 + -1.0 834,814 + -0.6 834,814 + -0.6 834,814 + -0.3 834,814 + +0.1 834,814 + +0.2 834,814 + -0.9 834,814 + +0.0 834,814 + -0.4 834,814 + +1.2 834,814 + -0.8 834,814 + -0.0 834,814 + +0.2 834,815 - +0.5 834,815 - -0.8 834,815 - -0.3 834,815 - +0.9 834,815 - -0.2 834,815 - -0.4 834,815 - -0.3 834,815 - +0.0 834,815 - +3.0 834,815 - -0.0 834,815 - -0.6 834,815 - -0.6 834,816 + -0.1 834,816 + -0.2 834,816 + -0.2 834,816 + -1.2 834,816 + -0.8 834,816 + -2.1 834,817 - +0.1 834,817 - +0.5 834,817 - +0.1 834,846 + +0.5 834,882 - -0.1 834,882 - -0.6 834,931 + AMB_RS03980 0.12 -0.4 834,931 + AMB_RS03980 0.12 -0.6 834,931 + AMB_RS03980 0.12 +0.2 834,932 - AMB_RS03980 0.12 -0.9 834,932 - AMB_RS03980 0.12 +0.6 835,240 + AMB_RS03980 0.51 +0.2 835,240 + AMB_RS03980 0.51 +0.2 835,240 + AMB_RS03980 0.51 -1.4 835,240 + AMB_RS03980 0.51 -0.1 835,240 + AMB_RS03980 0.51 -1.6 835,240 + AMB_RS03980 0.51 +0.8 835,240 + AMB_RS03980 0.51 +2.5 835,240 + AMB_RS03980 0.51 +0.1 835,241 - AMB_RS03980 0.51 +0.7 835,241 - AMB_RS03980 0.51 +0.4 835,241 - AMB_RS03980 0.51 +1.1 835,241 - AMB_RS03980 0.51 +0.4 835,241 - AMB_RS03980 0.51 -2.5 835,241 - AMB_RS03980 0.51 +0.1 835,241 - AMB_RS03980 0.51 +0.3 835,276 + AMB_RS03980 0.56 +1.0 835,276 + AMB_RS03980 0.56 +0.8 835,369 + AMB_RS03980 0.68 -0.1 835,369 + AMB_RS03980 0.68 +0.9 835,370 - AMB_RS03980 0.68 +0.3 835,370 - AMB_RS03980 0.68 -0.8 835,370 - AMB_RS03980 0.68 +0.0 835,617 + +0.1 837,268 + AMB_RS03990 0.24 +1.7 837,268 + AMB_RS03990 0.24 +1.4 837,269 - AMB_RS03990 0.24 -0.6 837,269 - AMB_RS03990 0.24 -0.5 837,269 - AMB_RS03990 0.24 -0.7 837,352 + AMB_RS03990 0.42 -0.6 837,760 - AMB_RS03995 0.13 -0.6 837,876 + AMB_RS03995 0.24 +0.5 837,876 + AMB_RS03995 0.24 -1.9 837,876 + AMB_RS03995 0.24 +0.4 837,877 - AMB_RS03995 0.24 +0.5 837,877 - AMB_RS03995 0.24 +0.4 837,877 - AMB_RS03995 0.24 -0.4 837,877 - AMB_RS03995 0.24 +1.0 837,929 + AMB_RS03995 0.29 -1.6 837,939 + AMB_RS03995 0.30 -0.4 837,940 - AMB_RS03995 0.30 -0.0 837,940 - AMB_RS03995 0.30 -0.4 837,940 - AMB_RS03995 0.30 -0.4 837,957 + AMB_RS03995 0.32 -0.3 838,112 + AMB_RS03995 0.46 -0.8
Or see this region's nucleotide sequence