Experiment: 30_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03050 and AMB_RS03055 are separated by 101 nucleotides AMB_RS03055 and AMB_RS03060 overlap by 4 nucleotides AMB_RS03060 and AMB_RS03065 are separated by 0 nucleotides
AMB_RS03050: AMB_RS03050 - ribose-phosphate pyrophosphokinase, at 638,226 to 639,158
_RS03050
AMB_RS03055: AMB_RS03055 - hypothetical protein, at 639,260 to 640,081
_RS03055
AMB_RS03060: AMB_RS03060 - peptidoglycan editing factor PgeF, at 640,078 to 640,848
_RS03060
AMB_RS03065: AMB_RS03065 - class I SAM-dependent methyltransferase, at 640,849 to 641,922
_RS03065
Position (kb) 639
640
641 Strain fitness (log2 ratio) -1
0
1 at 639.177 kb on - strand at 639.248 kb on - strand at 639.248 kb on - strand at 639.282 kb on - strand at 639.672 kb on + strand, within AMB_RS03055 at 639.672 kb on + strand, within AMB_RS03055 at 640.148 kb on + strand at 640.149 kb on - strand at 640.238 kb on + strand, within AMB_RS03060 at 640.238 kb on + strand, within AMB_RS03060 at 640.239 kb on - strand, within AMB_RS03060 at 640.352 kb on + strand, within AMB_RS03060 at 640.435 kb on - strand, within AMB_RS03060 at 640.851 kb on - strand at 640.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_1 remove 639,177 - +0.7 639,248 - -0.3 639,248 - +0.1 639,282 - +0.2 639,672 + AMB_RS03055 0.50 -1.2 639,672 + AMB_RS03055 0.50 +0.3 640,148 + -1.2 640,149 - -1.2 640,238 + AMB_RS03060 0.21 +0.9 640,238 + AMB_RS03060 0.21 +0.1 640,239 - AMB_RS03060 0.21 -0.5 640,352 + AMB_RS03060 0.36 +0.1 640,435 - AMB_RS03060 0.46 -1.2 640,851 - -0.6 640,851 - -0.8
Or see this region's nucleotide sequence