Experiment: 30_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01070 and AMB_RS01075 are separated by 259 nucleotides AMB_RS01075 and AMB_RS01080 overlap by 470 nucleotides
AMB_RS01070: AMB_RS01070 - hydrogenase 4 subunit B, at 236,293 to 238,302
_RS01070
AMB_RS01075: AMB_RS01075 - phosphoribosylamine--glycine ligase, at 238,562 to 239,836
_RS01075
AMB_RS01080: AMB_RS01080 - exodeoxyribonuclease VII large subunit, at 239,367 to 241,433
_RS01080
Position (kb)
238
239
240 Strain fitness (log2 ratio)
-2
-1
0
1 at 237.740 kb on + strand, within AMB_RS01070 at 237.740 kb on + strand, within AMB_RS01070 at 237.816 kb on - strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.965 kb on + strand, within AMB_RS01070 at 237.965 kb on + strand, within AMB_RS01070 at 237.966 kb on - strand, within AMB_RS01070 at 237.966 kb on - strand, within AMB_RS01070 at 237.966 kb on - strand, within AMB_RS01070 at 237.966 kb on - strand, within AMB_RS01070 at 238.019 kb on + strand, within AMB_RS01070 at 238.212 kb on - strand at 238.502 kb on + strand at 238.503 kb on - strand at 238.524 kb on + strand at 238.525 kb on - strand at 238.525 kb on - strand at 238.525 kb on - strand at 238.563 kb on + strand at 238.563 kb on + strand at 239.870 kb on - strand, within AMB_RS01080 at 239.870 kb on - strand, within AMB_RS01080 at 240.078 kb on + strand, within AMB_RS01080 at 240.078 kb on + strand, within AMB_RS01080 at 240.078 kb on + strand, within AMB_RS01080 at 240.079 kb on - strand, within AMB_RS01080
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_1 remove 237,740 + AMB_RS01070 0.72 +1.1 237,740 + AMB_RS01070 0.72 +0.2 237,816 - AMB_RS01070 0.76 -0.2 237,912 + AMB_RS01070 0.81 -0.6 237,912 + AMB_RS01070 0.81 +0.3 237,912 + AMB_RS01070 0.81 -0.2 237,912 + AMB_RS01070 0.81 -1.5 237,912 + AMB_RS01070 0.81 -0.2 237,913 - AMB_RS01070 0.81 +0.2 237,913 - AMB_RS01070 0.81 +0.3 237,913 - AMB_RS01070 0.81 -0.8 237,913 - AMB_RS01070 0.81 -1.3 237,913 - AMB_RS01070 0.81 -0.6 237,965 + AMB_RS01070 0.83 -0.3 237,965 + AMB_RS01070 0.83 -0.1 237,966 - AMB_RS01070 0.83 -0.8 237,966 - AMB_RS01070 0.83 -0.3 237,966 - AMB_RS01070 0.83 +0.4 237,966 - AMB_RS01070 0.83 -0.4 238,019 + AMB_RS01070 0.86 -0.2 238,212 - -2.1 238,502 + +0.3 238,503 - -1.2 238,524 + -0.2 238,525 - +0.6 238,525 - +0.4 238,525 - -0.6 238,563 + +0.1 238,563 + -0.4 239,870 - AMB_RS01080 0.24 -0.6 239,870 - AMB_RS01080 0.24 -2.4 240,078 + AMB_RS01080 0.34 -0.2 240,078 + AMB_RS01080 0.34 -0.7 240,078 + AMB_RS01080 0.34 +0.2 240,079 - AMB_RS01080 0.34 +0.4
Or see this region's nucleotide sequence