Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_17830 and IAI46_17835 are separated by 233 nucleotides IAI46_17835 and IAI46_17840 are separated by 75 nucleotides IAI46_17840 and IAI46_17845 overlap by 1 nucleotides
IAI46_17830: IAI46_17830 - acetyl-CoA carboxylase, carboxyltransferase subunit beta, at 3,757,474 to 3,758,382
_17830
IAI46_17835: IAI46_17835 - DedA family protein, at 3,758,616 to 3,759,278
_17835
IAI46_17840: IAI46_17840 - tRNA pseudouridine(38-40) synthase TruA, at 3,759,354 to 3,760,175
_17840
IAI46_17845: IAI46_17845 - aspartate-semialdehyde dehydrogenase, at 3,760,175 to 3,761,185
_17845
Position (kb)
3758
3759
3760 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3758.539 kb on - strand at 3758.761 kb on + strand, within IAI46_17835 at 3758.761 kb on + strand, within IAI46_17835 at 3758.762 kb on - strand, within IAI46_17835 at 3758.762 kb on - strand, within IAI46_17835 at 3758.762 kb on - strand, within IAI46_17835 at 3758.762 kb on - strand, within IAI46_17835 at 3758.762 kb on - strand, within IAI46_17835 at 3758.804 kb on - strand, within IAI46_17835 at 3758.821 kb on + strand, within IAI46_17835 at 3758.934 kb on - strand, within IAI46_17835 at 3759.005 kb on + strand, within IAI46_17835 at 3759.006 kb on - strand, within IAI46_17835 at 3759.006 kb on - strand, within IAI46_17835 at 3759.010 kb on + strand, within IAI46_17835 at 3759.010 kb on + strand, within IAI46_17835 at 3759.010 kb on + strand, within IAI46_17835 at 3759.010 kb on + strand, within IAI46_17835 at 3759.011 kb on - strand, within IAI46_17835 at 3759.011 kb on - strand, within IAI46_17835 at 3759.186 kb on - strand, within IAI46_17835 at 3759.190 kb on + strand, within IAI46_17835 at 3759.191 kb on - strand, within IAI46_17835 at 3759.191 kb on - strand, within IAI46_17835 at 3759.201 kb on - strand, within IAI46_17835 at 3759.201 kb on - strand, within IAI46_17835 at 3759.205 kb on + strand, within IAI46_17835 at 3759.205 kb on + strand, within IAI46_17835 at 3759.206 kb on - strand, within IAI46_17835 at 3759.239 kb on + strand at 3759.278 kb on + strand at 3759.278 kb on + strand at 3759.279 kb on - strand at 3759.319 kb on + strand at 3759.319 kb on + strand at 3759.319 kb on + strand at 3759.320 kb on - strand at 3759.415 kb on + strand at 3759.416 kb on - strand at 3759.433 kb on + strand at 3759.433 kb on + strand at 3759.434 kb on - strand at 3759.563 kb on + strand, within IAI46_17840 at 3759.563 kb on + strand, within IAI46_17840 at 3759.564 kb on - strand, within IAI46_17840 at 3759.564 kb on - strand, within IAI46_17840 at 3759.590 kb on + strand, within IAI46_17840 at 3759.590 kb on + strand, within IAI46_17840 at 3759.595 kb on - strand, within IAI46_17840 at 3759.602 kb on + strand, within IAI46_17840 at 3759.602 kb on + strand, within IAI46_17840 at 3759.603 kb on - strand, within IAI46_17840 at 3759.603 kb on - strand, within IAI46_17840 at 3759.603 kb on - strand, within IAI46_17840 at 3759.603 kb on - strand, within IAI46_17840 at 3759.627 kb on - strand, within IAI46_17840 at 3759.627 kb on - strand, within IAI46_17840 at 3759.637 kb on - strand, within IAI46_17840 at 3759.686 kb on + strand, within IAI46_17840 at 3759.777 kb on + strand, within IAI46_17840 at 3759.806 kb on + strand, within IAI46_17840 at 3759.806 kb on + strand, within IAI46_17840 at 3759.807 kb on - strand, within IAI46_17840 at 3759.807 kb on - strand, within IAI46_17840 at 3759.995 kb on + strand, within IAI46_17840 at 3760.100 kb on - strand at 3760.118 kb on - strand at 3760.179 kb on + strand at 3760.179 kb on + strand at 3760.180 kb on - strand at 3760.180 kb on - strand at 3760.180 kb on - strand at 3760.180 kb on - strand at 3760.241 kb on + strand at 3760.241 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 3,758,539 - +0.8 3,758,761 + IAI46_17835 0.22 -0.1 3,758,761 + IAI46_17835 0.22 +0.8 3,758,762 - IAI46_17835 0.22 +0.5 3,758,762 - IAI46_17835 0.22 +0.3 3,758,762 - IAI46_17835 0.22 +0.7 3,758,762 - IAI46_17835 0.22 +2.4 3,758,762 - IAI46_17835 0.22 -0.3 3,758,804 - IAI46_17835 0.28 -0.2 3,758,821 + IAI46_17835 0.31 -0.0 3,758,934 - IAI46_17835 0.48 -0.0 3,759,005 + IAI46_17835 0.59 +0.6 3,759,006 - IAI46_17835 0.59 +0.9 3,759,006 - IAI46_17835 0.59 +0.9 3,759,010 + IAI46_17835 0.59 -0.2 3,759,010 + IAI46_17835 0.59 +0.5 3,759,010 + IAI46_17835 0.59 +0.7 3,759,010 + IAI46_17835 0.59 +1.1 3,759,011 - IAI46_17835 0.60 +1.8 3,759,011 - IAI46_17835 0.60 +0.1 3,759,186 - IAI46_17835 0.86 -0.6 3,759,190 + IAI46_17835 0.87 +0.1 3,759,191 - IAI46_17835 0.87 -1.1 3,759,191 - IAI46_17835 0.87 +0.2 3,759,201 - IAI46_17835 0.88 -0.0 3,759,201 - IAI46_17835 0.88 -0.1 3,759,205 + IAI46_17835 0.89 -0.5 3,759,205 + IAI46_17835 0.89 -0.7 3,759,206 - IAI46_17835 0.89 +1.6 3,759,239 + -1.2 3,759,278 + -0.7 3,759,278 + -0.4 3,759,279 - -0.2 3,759,319 + +0.7 3,759,319 + +0.4 3,759,319 + +0.3 3,759,320 - +0.6 3,759,415 + +0.4 3,759,416 - +0.8 3,759,433 + +1.8 3,759,433 + +0.5 3,759,434 - -0.5 3,759,563 + IAI46_17840 0.25 +1.0 3,759,563 + IAI46_17840 0.25 +1.0 3,759,564 - IAI46_17840 0.26 -0.4 3,759,564 - IAI46_17840 0.26 -0.3 3,759,590 + IAI46_17840 0.29 -2.0 3,759,590 + IAI46_17840 0.29 -0.6 3,759,595 - IAI46_17840 0.29 -1.9 3,759,602 + IAI46_17840 0.30 +0.8 3,759,602 + IAI46_17840 0.30 -0.7 3,759,603 - IAI46_17840 0.30 -1.4 3,759,603 - IAI46_17840 0.30 +0.2 3,759,603 - IAI46_17840 0.30 -0.2 3,759,603 - IAI46_17840 0.30 -0.5 3,759,627 - IAI46_17840 0.33 -0.9 3,759,627 - IAI46_17840 0.33 -1.0 3,759,637 - IAI46_17840 0.34 -1.9 3,759,686 + IAI46_17840 0.40 -2.0 3,759,777 + IAI46_17840 0.51 -0.3 3,759,806 + IAI46_17840 0.55 -1.0 3,759,806 + IAI46_17840 0.55 -0.5 3,759,807 - IAI46_17840 0.55 +0.4 3,759,807 - IAI46_17840 0.55 -0.0 3,759,995 + IAI46_17840 0.78 -2.6 3,760,100 - -0.4 3,760,118 - -0.4 3,760,179 + +1.2 3,760,179 + +0.1 3,760,180 - -0.9 3,760,180 - +0.3 3,760,180 - +0.8 3,760,180 - -1.1 3,760,241 + -0.5 3,760,241 + -1.1
Or see this region's nucleotide sequence