Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_08965 and IAI46_08970 overlap by 4 nucleotides IAI46_08970 and IAI46_08975 are separated by 15 nucleotides IAI46_08975 and IAI46_08980 are separated by 409 nucleotides
IAI46_08965: IAI46_08965 - c-type cytochrome, at 1,922,994 to 1,924,403
_08965
IAI46_08970: IAI46_08970 - GMC family oxidoreductase, at 1,924,400 to 1,926,055
_08970
IAI46_08975: IAI46_08975 - sorbitol dehydrogenase family protein, at 1,926,071 to 1,926,631
_08975
IAI46_08980: IAI46_08980 - DNA/RNA non-specific endonuclease, at 1,927,041 to 1,927,838
_08980
Position (kb)
1924
1925
1926
1927 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1923.414 kb on + strand, within IAI46_08965 at 1923.848 kb on - strand, within IAI46_08965 at 1923.848 kb on - strand, within IAI46_08965 at 1924.007 kb on + strand, within IAI46_08965 at 1924.013 kb on - strand, within IAI46_08965 at 1924.018 kb on + strand, within IAI46_08965 at 1924.053 kb on - strand, within IAI46_08965 at 1924.054 kb on + strand, within IAI46_08965 at 1924.054 kb on + strand, within IAI46_08965 at 1924.055 kb on - strand, within IAI46_08965 at 1924.055 kb on - strand, within IAI46_08965 at 1924.061 kb on + strand, within IAI46_08965 at 1924.061 kb on + strand, within IAI46_08965 at 1924.061 kb on + strand, within IAI46_08965 at 1924.062 kb on - strand, within IAI46_08965 at 1924.062 kb on - strand, within IAI46_08965 at 1924.063 kb on + strand, within IAI46_08965 at 1924.064 kb on - strand, within IAI46_08965 at 1924.140 kb on - strand, within IAI46_08965 at 1924.141 kb on + strand, within IAI46_08965 at 1924.187 kb on - strand, within IAI46_08965 at 1924.212 kb on - strand, within IAI46_08965 at 1924.253 kb on + strand, within IAI46_08965 at 1924.280 kb on + strand at 1924.280 kb on + strand at 1924.280 kb on + strand at 1924.281 kb on - strand at 1924.375 kb on - strand at 1924.375 kb on - strand at 1924.377 kb on + strand at 1924.378 kb on - strand at 1924.610 kb on - strand, within IAI46_08970 at 1924.681 kb on + strand, within IAI46_08970 at 1924.682 kb on - strand, within IAI46_08970 at 1924.702 kb on + strand, within IAI46_08970 at 1924.711 kb on - strand, within IAI46_08970 at 1924.725 kb on + strand, within IAI46_08970 at 1924.725 kb on + strand, within IAI46_08970 at 1924.726 kb on - strand, within IAI46_08970 at 1924.789 kb on + strand, within IAI46_08970 at 1924.790 kb on - strand, within IAI46_08970 at 1924.946 kb on - strand, within IAI46_08970 at 1924.956 kb on + strand, within IAI46_08970 at 1925.050 kb on + strand, within IAI46_08970 at 1925.267 kb on + strand, within IAI46_08970 at 1925.267 kb on + strand, within IAI46_08970 at 1925.268 kb on - strand, within IAI46_08970 at 1925.268 kb on - strand, within IAI46_08970 at 1925.343 kb on + strand, within IAI46_08970 at 1925.343 kb on + strand, within IAI46_08970 at 1925.344 kb on - strand, within IAI46_08970 at 1925.344 kb on - strand, within IAI46_08970 at 1925.485 kb on + strand, within IAI46_08970 at 1925.622 kb on + strand, within IAI46_08970 at 1925.622 kb on + strand, within IAI46_08970 at 1925.623 kb on - strand, within IAI46_08970 at 1925.623 kb on - strand, within IAI46_08970 at 1925.652 kb on + strand, within IAI46_08970 at 1925.735 kb on + strand, within IAI46_08970 at 1925.735 kb on + strand, within IAI46_08970 at 1925.735 kb on + strand, within IAI46_08970 at 1925.735 kb on + strand, within IAI46_08970 at 1925.735 kb on + strand, within IAI46_08970 at 1925.736 kb on - strand, within IAI46_08970 at 1925.736 kb on - strand, within IAI46_08970 at 1925.774 kb on + strand, within IAI46_08970 at 1925.785 kb on - strand, within IAI46_08970 at 1925.802 kb on + strand, within IAI46_08970 at 1925.836 kb on - strand, within IAI46_08970 at 1925.878 kb on + strand, within IAI46_08970 at 1925.914 kb on + strand at 1926.037 kb on + strand at 1926.133 kb on - strand, within IAI46_08975 at 1926.168 kb on + strand, within IAI46_08975 at 1926.185 kb on - strand, within IAI46_08975 at 1926.186 kb on + strand, within IAI46_08975 at 1926.186 kb on + strand, within IAI46_08975 at 1926.320 kb on + strand, within IAI46_08975 at 1926.321 kb on - strand, within IAI46_08975 at 1926.371 kb on - strand, within IAI46_08975 at 1926.374 kb on + strand, within IAI46_08975 at 1926.374 kb on + strand, within IAI46_08975 at 1926.374 kb on + strand, within IAI46_08975 at 1926.375 kb on - strand, within IAI46_08975 at 1926.375 kb on - strand, within IAI46_08975 at 1926.375 kb on - strand, within IAI46_08975 at 1926.375 kb on - strand, within IAI46_08975 at 1926.375 kb on - strand, within IAI46_08975 at 1926.375 kb on - strand, within IAI46_08975 at 1926.513 kb on + strand, within IAI46_08975 at 1926.514 kb on - strand, within IAI46_08975 at 1926.717 kb on + strand at 1926.889 kb on + strand at 1926.896 kb on - strand at 1926.899 kb on + strand at 1926.899 kb on + strand at 1926.912 kb on + strand at 1926.918 kb on + strand at 1926.962 kb on - strand at 1926.969 kb on - strand at 1927.006 kb on + strand at 1927.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 1,923,414 + IAI46_08965 0.30 +0.6 1,923,848 - IAI46_08965 0.61 -1.1 1,923,848 - IAI46_08965 0.61 +0.8 1,924,007 + IAI46_08965 0.72 +0.6 1,924,013 - IAI46_08965 0.72 +1.1 1,924,018 + IAI46_08965 0.73 +1.2 1,924,053 - IAI46_08965 0.75 +0.2 1,924,054 + IAI46_08965 0.75 +0.4 1,924,054 + IAI46_08965 0.75 -0.5 1,924,055 - IAI46_08965 0.75 -0.8 1,924,055 - IAI46_08965 0.75 +1.9 1,924,061 + IAI46_08965 0.76 -0.4 1,924,061 + IAI46_08965 0.76 +0.4 1,924,061 + IAI46_08965 0.76 -0.1 1,924,062 - IAI46_08965 0.76 +0.7 1,924,062 - IAI46_08965 0.76 -0.3 1,924,063 + IAI46_08965 0.76 +0.0 1,924,064 - IAI46_08965 0.76 -0.1 1,924,140 - IAI46_08965 0.81 +0.7 1,924,141 + IAI46_08965 0.81 +0.4 1,924,187 - IAI46_08965 0.85 +0.7 1,924,212 - IAI46_08965 0.86 -0.1 1,924,253 + IAI46_08965 0.89 -0.2 1,924,280 + +1.6 1,924,280 + -1.6 1,924,280 + -1.9 1,924,281 - -0.4 1,924,375 - -0.4 1,924,375 - -0.5 1,924,377 + -0.4 1,924,378 - +0.6 1,924,610 - IAI46_08970 0.13 +0.9 1,924,681 + IAI46_08970 0.17 +0.5 1,924,682 - IAI46_08970 0.17 -1.5 1,924,702 + IAI46_08970 0.18 +0.6 1,924,711 - IAI46_08970 0.19 +0.7 1,924,725 + IAI46_08970 0.20 -0.1 1,924,725 + IAI46_08970 0.20 -1.8 1,924,726 - IAI46_08970 0.20 -0.2 1,924,789 + IAI46_08970 0.23 -0.7 1,924,790 - IAI46_08970 0.24 +0.3 1,924,946 - IAI46_08970 0.33 -0.1 1,924,956 + IAI46_08970 0.34 -0.2 1,925,050 + IAI46_08970 0.39 +0.3 1,925,267 + IAI46_08970 0.52 -0.9 1,925,267 + IAI46_08970 0.52 +0.1 1,925,268 - IAI46_08970 0.52 -0.2 1,925,268 - IAI46_08970 0.52 +0.5 1,925,343 + IAI46_08970 0.57 +1.6 1,925,343 + IAI46_08970 0.57 +0.4 1,925,344 - IAI46_08970 0.57 +0.4 1,925,344 - IAI46_08970 0.57 -1.6 1,925,485 + IAI46_08970 0.66 -0.2 1,925,622 + IAI46_08970 0.74 +0.3 1,925,622 + IAI46_08970 0.74 -0.7 1,925,623 - IAI46_08970 0.74 +0.0 1,925,623 - IAI46_08970 0.74 +0.9 1,925,652 + IAI46_08970 0.76 +0.0 1,925,735 + IAI46_08970 0.81 +1.1 1,925,735 + IAI46_08970 0.81 +0.6 1,925,735 + IAI46_08970 0.81 +0.4 1,925,735 + IAI46_08970 0.81 +1.2 1,925,735 + IAI46_08970 0.81 -0.5 1,925,736 - IAI46_08970 0.81 +0.4 1,925,736 - IAI46_08970 0.81 -0.2 1,925,774 + IAI46_08970 0.83 +0.9 1,925,785 - IAI46_08970 0.84 +0.3 1,925,802 + IAI46_08970 0.85 -0.2 1,925,836 - IAI46_08970 0.87 -2.0 1,925,878 + IAI46_08970 0.89 +0.6 1,925,914 + -1.2 1,926,037 + -0.1 1,926,133 - IAI46_08975 0.11 -0.4 1,926,168 + IAI46_08975 0.17 -1.0 1,926,185 - IAI46_08975 0.20 +0.6 1,926,186 + IAI46_08975 0.20 -1.3 1,926,186 + IAI46_08975 0.20 -0.9 1,926,320 + IAI46_08975 0.44 +1.0 1,926,321 - IAI46_08975 0.45 -0.8 1,926,371 - IAI46_08975 0.53 -0.7 1,926,374 + IAI46_08975 0.54 +0.3 1,926,374 + IAI46_08975 0.54 +0.2 1,926,374 + IAI46_08975 0.54 +0.5 1,926,375 - IAI46_08975 0.54 +0.3 1,926,375 - IAI46_08975 0.54 -0.7 1,926,375 - IAI46_08975 0.54 -0.7 1,926,375 - IAI46_08975 0.54 -0.2 1,926,375 - IAI46_08975 0.54 +0.3 1,926,375 - IAI46_08975 0.54 +0.4 1,926,513 + IAI46_08975 0.79 -1.2 1,926,514 - IAI46_08975 0.79 +0.2 1,926,717 + -1.0 1,926,889 + +0.4 1,926,896 - +0.5 1,926,899 + +0.1 1,926,899 + -1.0 1,926,912 + +0.2 1,926,918 + +1.0 1,926,962 - +0.3 1,926,969 - -0.6 1,927,006 + +0.6 1,927,043 - -0.1
Or see this region's nucleotide sequence