Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_05295 and IAI46_05300 are separated by 47 nucleotides IAI46_05300 and IAI46_05305 are separated by 225 nucleotides IAI46_05305 and IAI46_05310 are separated by 149 nucleotides
IAI46_05295: IAI46_05295 - isoprenylcysteine carboxylmethyltransferase family protein, at 1,141,545 to 1,142,285
_05295
IAI46_05300: IAI46_05300 - Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK, at 1,142,333 to 1,142,812
_05300
IAI46_05305: IAI46_05305 - TraB/GumN family protein, at 1,143,038 to 1,143,841
_05305
IAI46_05310: IAI46_05310 - copper-exporting P-type ATPase CopA, at 1,143,991 to 1,146,711
_05310
Position (kb)
1143
1144 Strain fitness (log2 ratio)
-2
-1
0
1 at 1142.087 kb on - strand, within IAI46_05295 at 1142.145 kb on - strand, within IAI46_05295 at 1142.145 kb on - strand, within IAI46_05295 at 1142.152 kb on + strand, within IAI46_05295 at 1142.153 kb on - strand, within IAI46_05295 at 1142.153 kb on - strand, within IAI46_05295 at 1142.271 kb on - strand at 1142.283 kb on + strand at 1142.284 kb on - strand at 1142.334 kb on + strand at 1142.334 kb on + strand at 1142.433 kb on + strand, within IAI46_05300 at 1142.433 kb on + strand, within IAI46_05300 at 1142.433 kb on + strand, within IAI46_05300 at 1142.433 kb on + strand, within IAI46_05300 at 1142.433 kb on + strand, within IAI46_05300 at 1142.433 kb on + strand, within IAI46_05300 at 1142.434 kb on - strand, within IAI46_05300 at 1142.434 kb on - strand, within IAI46_05300 at 1142.611 kb on + strand, within IAI46_05300 at 1142.611 kb on + strand, within IAI46_05300 at 1142.611 kb on + strand, within IAI46_05300 at 1142.612 kb on - strand, within IAI46_05300 at 1142.612 kb on - strand, within IAI46_05300 at 1142.615 kb on + strand, within IAI46_05300 at 1142.677 kb on + strand, within IAI46_05300 at 1142.677 kb on + strand, within IAI46_05300 at 1142.677 kb on + strand, within IAI46_05300 at 1142.767 kb on + strand at 1142.973 kb on + strand at 1142.973 kb on + strand at 1142.974 kb on - strand at 1142.979 kb on + strand at 1142.979 kb on + strand at 1142.979 kb on + strand at 1142.979 kb on + strand at 1142.980 kb on - strand at 1142.980 kb on - strand at 1142.980 kb on - strand at 1142.980 kb on - strand at 1142.980 kb on - strand at 1142.980 kb on - strand at 1142.980 kb on - strand at 1142.981 kb on + strand at 1142.981 kb on + strand at 1142.982 kb on - strand at 1142.986 kb on - strand at 1142.990 kb on + strand at 1142.991 kb on - strand at 1143.068 kb on - strand at 1143.078 kb on + strand at 1143.122 kb on + strand, within IAI46_05305 at 1143.178 kb on - strand, within IAI46_05305 at 1143.312 kb on - strand, within IAI46_05305 at 1143.312 kb on - strand, within IAI46_05305 at 1143.382 kb on + strand, within IAI46_05305 at 1143.383 kb on - strand, within IAI46_05305 at 1143.479 kb on + strand, within IAI46_05305 at 1143.546 kb on - strand, within IAI46_05305 at 1143.700 kb on + strand, within IAI46_05305 at 1143.700 kb on + strand, within IAI46_05305 at 1143.701 kb on - strand, within IAI46_05305 at 1143.716 kb on - strand, within IAI46_05305 at 1143.763 kb on + strand at 1143.783 kb on + strand at 1143.783 kb on + strand at 1143.783 kb on + strand at 1143.784 kb on - strand at 1143.842 kb on - strand at 1143.903 kb on + strand at 1143.903 kb on + strand at 1143.903 kb on + strand at 1143.972 kb on - strand at 1143.972 kb on - strand at 1143.988 kb on + strand at 1143.988 kb on + strand at 1143.989 kb on - strand at 1143.992 kb on + strand at 1143.993 kb on - strand at 1144.042 kb on - strand at 1144.042 kb on - strand at 1144.060 kb on - strand at 1144.060 kb on - strand at 1144.106 kb on + strand at 1144.106 kb on + strand at 1144.145 kb on + strand at 1144.176 kb on + strand at 1144.194 kb on + strand at 1144.241 kb on - strand at 1144.333 kb on - strand, within IAI46_05310 at 1144.497 kb on - strand, within IAI46_05310 at 1144.497 kb on - strand, within IAI46_05310 at 1144.578 kb on + strand, within IAI46_05310 at 1144.605 kb on + strand, within IAI46_05310 at 1144.606 kb on - strand, within IAI46_05310 at 1144.743 kb on + strand, within IAI46_05310
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 1,142,087 - IAI46_05295 0.73 +0.3 1,142,145 - IAI46_05295 0.81 +1.0 1,142,145 - IAI46_05295 0.81 +1.2 1,142,152 + IAI46_05295 0.82 +1.1 1,142,153 - IAI46_05295 0.82 -0.2 1,142,153 - IAI46_05295 0.82 +0.1 1,142,271 - +0.1 1,142,283 + +0.4 1,142,284 - +0.3 1,142,334 + +1.0 1,142,334 + -0.1 1,142,433 + IAI46_05300 0.21 -0.6 1,142,433 + IAI46_05300 0.21 -1.1 1,142,433 + IAI46_05300 0.21 -0.1 1,142,433 + IAI46_05300 0.21 +1.5 1,142,433 + IAI46_05300 0.21 +0.3 1,142,433 + IAI46_05300 0.21 -0.0 1,142,434 - IAI46_05300 0.21 +1.1 1,142,434 - IAI46_05300 0.21 +0.4 1,142,611 + IAI46_05300 0.58 -0.7 1,142,611 + IAI46_05300 0.58 -0.1 1,142,611 + IAI46_05300 0.58 -0.2 1,142,612 - IAI46_05300 0.58 +0.6 1,142,612 - IAI46_05300 0.58 -0.4 1,142,615 + IAI46_05300 0.59 -0.6 1,142,677 + IAI46_05300 0.72 +1.0 1,142,677 + IAI46_05300 0.72 -0.8 1,142,677 + IAI46_05300 0.72 -0.1 1,142,767 + +1.4 1,142,973 + +0.2 1,142,973 + -0.7 1,142,974 - +0.8 1,142,979 + +0.1 1,142,979 + +0.1 1,142,979 + +0.9 1,142,979 + -0.5 1,142,980 - -0.2 1,142,980 - -0.5 1,142,980 - +0.1 1,142,980 - -0.7 1,142,980 - -0.7 1,142,980 - -0.1 1,142,980 - +1.1 1,142,981 + -0.2 1,142,981 + +0.6 1,142,982 - -0.1 1,142,986 - -0.2 1,142,990 + +0.5 1,142,991 - -0.2 1,143,068 - -2.0 1,143,078 + +0.1 1,143,122 + IAI46_05305 0.10 -1.7 1,143,178 - IAI46_05305 0.17 -0.6 1,143,312 - IAI46_05305 0.34 -0.5 1,143,312 - IAI46_05305 0.34 +0.4 1,143,382 + IAI46_05305 0.43 -0.0 1,143,383 - IAI46_05305 0.43 +0.3 1,143,479 + IAI46_05305 0.55 +1.1 1,143,546 - IAI46_05305 0.63 +0.8 1,143,700 + IAI46_05305 0.82 +0.4 1,143,700 + IAI46_05305 0.82 +0.7 1,143,701 - IAI46_05305 0.82 -0.6 1,143,716 - IAI46_05305 0.84 +0.3 1,143,763 + +0.3 1,143,783 + -0.1 1,143,783 + +0.9 1,143,783 + +1.5 1,143,784 - +0.1 1,143,842 - -0.7 1,143,903 + +0.1 1,143,903 + +0.2 1,143,903 + +0.2 1,143,972 - +0.9 1,143,972 - +0.2 1,143,988 + -0.1 1,143,988 + -0.5 1,143,989 - +0.6 1,143,992 + +0.6 1,143,993 - +0.2 1,144,042 - +0.5 1,144,042 - +0.7 1,144,060 - -0.3 1,144,060 - +0.3 1,144,106 + +0.6 1,144,106 + -0.3 1,144,145 + +1.1 1,144,176 + -0.8 1,144,194 + -0.3 1,144,241 - -0.3 1,144,333 - IAI46_05310 0.13 +0.6 1,144,497 - IAI46_05310 0.19 +0.4 1,144,497 - IAI46_05310 0.19 +1.0 1,144,578 + IAI46_05310 0.22 +0.3 1,144,605 + IAI46_05310 0.23 +0.8 1,144,606 - IAI46_05310 0.23 -2.5 1,144,743 + IAI46_05310 0.28 +0.0
Or see this region's nucleotide sequence