Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_04940 and IAI46_04945 are separated by 33 nucleotides IAI46_04945 and IAI46_04950 overlap by 38 nucleotides IAI46_04950 and IAI46_04955 are separated by 167 nucleotides IAI46_04955 and IAI46_04960 are separated by 201 nucleotides
IAI46_04940: IAI46_04940 - tRNA 4-thiouridine(8) synthase ThiI, at 1,063,951 to 1,065,399
_04940
IAI46_04945: IAI46_04945 - protein deglycase YajL, at 1,065,433 to 1,066,023
_04945
IAI46_04950: IAI46_04950 - 2-dehydropantoate 2-reductase, at 1,065,986 to 1,066,897
_04950
IAI46_04955: IAI46_04955 - YajQ family cyclic di-GMP-binding protein, at 1,067,065 to 1,067,556
_04955
IAI46_04960: IAI46_04960 - MFS transporter, at 1,067,758 to 1,069,122
_04960
Position (kb)
1065
1066
1067 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1065.196 kb on - strand, within IAI46_04940 at 1065.260 kb on - strand at 1065.261 kb on + strand at 1065.304 kb on + strand at 1065.304 kb on + strand at 1065.305 kb on - strand at 1065.314 kb on - strand at 1065.353 kb on - strand at 1065.371 kb on - strand at 1065.371 kb on - strand at 1065.511 kb on + strand, within IAI46_04945 at 1065.574 kb on - strand, within IAI46_04945 at 1065.588 kb on - strand, within IAI46_04945 at 1065.588 kb on - strand, within IAI46_04945 at 1065.590 kb on - strand, within IAI46_04945 at 1065.654 kb on + strand, within IAI46_04945 at 1065.655 kb on - strand, within IAI46_04945 at 1065.655 kb on - strand, within IAI46_04945 at 1065.760 kb on + strand, within IAI46_04945 at 1065.761 kb on - strand, within IAI46_04945 at 1065.841 kb on - strand, within IAI46_04945 at 1065.891 kb on + strand, within IAI46_04945 at 1065.891 kb on + strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.892 kb on - strand, within IAI46_04945 at 1065.924 kb on + strand, within IAI46_04945 at 1065.959 kb on - strand, within IAI46_04945 at 1065.988 kb on - strand at 1066.024 kb on - strand at 1066.024 kb on - strand at 1066.110 kb on + strand, within IAI46_04950 at 1066.123 kb on - strand, within IAI46_04950 at 1066.123 kb on - strand, within IAI46_04950 at 1066.209 kb on + strand, within IAI46_04950 at 1066.209 kb on + strand, within IAI46_04950 at 1066.210 kb on - strand, within IAI46_04950 at 1066.210 kb on - strand, within IAI46_04950 at 1066.234 kb on + strand, within IAI46_04950 at 1066.234 kb on + strand, within IAI46_04950 at 1066.234 kb on + strand, within IAI46_04950 at 1066.235 kb on - strand, within IAI46_04950 at 1066.235 kb on - strand, within IAI46_04950 at 1066.235 kb on - strand, within IAI46_04950 at 1066.239 kb on + strand, within IAI46_04950 at 1066.240 kb on - strand, within IAI46_04950 at 1066.327 kb on + strand, within IAI46_04950 at 1066.327 kb on + strand, within IAI46_04950 at 1066.329 kb on + strand, within IAI46_04950 at 1066.329 kb on + strand, within IAI46_04950 at 1066.330 kb on - strand, within IAI46_04950 at 1066.330 kb on - strand, within IAI46_04950 at 1066.330 kb on - strand, within IAI46_04950 at 1066.348 kb on + strand, within IAI46_04950 at 1066.349 kb on - strand, within IAI46_04950 at 1066.510 kb on + strand, within IAI46_04950 at 1066.513 kb on + strand, within IAI46_04950 at 1066.513 kb on + strand, within IAI46_04950 at 1066.513 kb on + strand, within IAI46_04950 at 1066.513 kb on + strand, within IAI46_04950 at 1066.514 kb on - strand, within IAI46_04950 at 1066.514 kb on - strand, within IAI46_04950 at 1066.685 kb on + strand, within IAI46_04950 at 1066.809 kb on - strand at 1066.820 kb on + strand at 1066.820 kb on + strand at 1066.821 kb on - strand at 1066.840 kb on - strand at 1066.890 kb on - strand at 1066.890 kb on - strand at 1066.890 kb on - strand at 1066.933 kb on - strand at 1066.936 kb on + strand at 1066.941 kb on + strand at 1066.947 kb on + strand at 1066.997 kb on + strand at 1067.039 kb on + strand at 1067.039 kb on + strand at 1067.064 kb on + strand at 1067.064 kb on + strand at 1067.064 kb on + strand at 1067.097 kb on + strand at 1067.097 kb on + strand at 1067.112 kb on + strand at 1067.113 kb on - strand at 1067.253 kb on + strand, within IAI46_04955 at 1067.367 kb on + strand, within IAI46_04955 at 1067.466 kb on + strand, within IAI46_04955 at 1067.575 kb on + strand at 1067.576 kb on - strand at 1067.576 kb on - strand at 1067.577 kb on + strand at 1067.578 kb on - strand at 1067.704 kb on + strand at 1067.760 kb on - strand at 1067.819 kb on - strand at 1067.823 kb on - strand at 1067.860 kb on + strand at 1067.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 1,065,196 - IAI46_04940 0.86 +0.3 1,065,260 - +0.1 1,065,261 + -1.4 1,065,304 + -0.3 1,065,304 + +1.1 1,065,305 - -0.3 1,065,314 - +0.5 1,065,353 - -0.1 1,065,371 - -0.8 1,065,371 - -0.2 1,065,511 + IAI46_04945 0.13 +0.4 1,065,574 - IAI46_04945 0.24 +0.2 1,065,588 - IAI46_04945 0.26 +0.6 1,065,588 - IAI46_04945 0.26 +0.1 1,065,590 - IAI46_04945 0.27 +0.6 1,065,654 + IAI46_04945 0.37 -0.3 1,065,655 - IAI46_04945 0.38 +0.8 1,065,655 - IAI46_04945 0.38 +0.3 1,065,760 + IAI46_04945 0.55 +1.8 1,065,761 - IAI46_04945 0.55 -0.7 1,065,841 - IAI46_04945 0.69 +0.5 1,065,891 + IAI46_04945 0.77 -0.5 1,065,891 + IAI46_04945 0.77 +0.8 1,065,892 - IAI46_04945 0.78 -0.0 1,065,892 - IAI46_04945 0.78 -1.1 1,065,892 - IAI46_04945 0.78 +1.5 1,065,892 - IAI46_04945 0.78 +1.6 1,065,892 - IAI46_04945 0.78 +2.9 1,065,892 - IAI46_04945 0.78 +0.1 1,065,892 - IAI46_04945 0.78 -0.1 1,065,924 + IAI46_04945 0.83 -0.3 1,065,959 - IAI46_04945 0.89 +0.7 1,065,988 - +0.3 1,066,024 - -1.3 1,066,024 - +1.1 1,066,110 + IAI46_04950 0.14 +0.5 1,066,123 - IAI46_04950 0.15 -0.1 1,066,123 - IAI46_04950 0.15 -0.9 1,066,209 + IAI46_04950 0.24 -0.1 1,066,209 + IAI46_04950 0.24 -0.3 1,066,210 - IAI46_04950 0.25 +0.6 1,066,210 - IAI46_04950 0.25 -1.1 1,066,234 + IAI46_04950 0.27 +0.2 1,066,234 + IAI46_04950 0.27 +0.1 1,066,234 + IAI46_04950 0.27 +0.1 1,066,235 - IAI46_04950 0.27 +0.1 1,066,235 - IAI46_04950 0.27 +0.3 1,066,235 - IAI46_04950 0.27 -0.3 1,066,239 + IAI46_04950 0.28 -0.4 1,066,240 - IAI46_04950 0.28 +0.2 1,066,327 + IAI46_04950 0.37 +0.0 1,066,327 + IAI46_04950 0.37 +0.1 1,066,329 + IAI46_04950 0.38 +0.2 1,066,329 + IAI46_04950 0.38 -0.1 1,066,330 - IAI46_04950 0.38 +0.9 1,066,330 - IAI46_04950 0.38 +0.3 1,066,330 - IAI46_04950 0.38 -0.3 1,066,348 + IAI46_04950 0.40 +0.5 1,066,349 - IAI46_04950 0.40 +0.8 1,066,510 + IAI46_04950 0.57 +0.4 1,066,513 + IAI46_04950 0.58 +0.8 1,066,513 + IAI46_04950 0.58 -0.9 1,066,513 + IAI46_04950 0.58 +0.6 1,066,513 + IAI46_04950 0.58 +0.0 1,066,514 - IAI46_04950 0.58 -0.1 1,066,514 - IAI46_04950 0.58 +1.2 1,066,685 + IAI46_04950 0.77 +0.1 1,066,809 - +0.8 1,066,820 + -0.1 1,066,820 + +0.8 1,066,821 - -0.7 1,066,840 - +0.1 1,066,890 - -1.3 1,066,890 - +0.9 1,066,890 - -0.0 1,066,933 - +0.8 1,066,936 + -0.3 1,066,941 + +0.0 1,066,947 + +0.3 1,066,997 + -0.4 1,067,039 + -1.3 1,067,039 + -0.4 1,067,064 + +0.7 1,067,064 + -0.1 1,067,064 + -0.4 1,067,097 + -0.2 1,067,097 + +0.1 1,067,112 + +0.1 1,067,113 - +0.4 1,067,253 + IAI46_04955 0.38 -0.1 1,067,367 + IAI46_04955 0.61 +0.4 1,067,466 + IAI46_04955 0.82 +0.2 1,067,575 + -0.1 1,067,576 - -0.6 1,067,576 - +0.2 1,067,577 + +1.3 1,067,578 - +0.6 1,067,704 + +0.4 1,067,760 - +1.1 1,067,819 - +0.4 1,067,823 - +0.2 1,067,860 + +0.1 1,067,861 - -0.8
Or see this region's nucleotide sequence