Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_01350 and IAI46_01355 are separated by 69 nucleotides IAI46_01355 and IAI46_01360 are separated by 45 nucleotides IAI46_01360 and IAI46_01365 are separated by 48 nucleotides
IAI46_01350: IAI46_01350 - DNA-binding protein HU-alpha, at 296,048 to 296,320
_01350
IAI46_01355: IAI46_01355 - DUF1481 domain-containing protein, at 296,390 to 297,028
_01355
IAI46_01360: IAI46_01360 - phosphoribosylamine--glycine ligase, at 297,074 to 298,357
_01360
IAI46_01365: IAI46_01365 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 298,406 to 299,995
_01365
Position (kb) 297
298
299 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 296.110 kb on + strand, within IAI46_01350 at 296.140 kb on + strand, within IAI46_01350 at 296.143 kb on + strand, within IAI46_01350 at 296.144 kb on - strand, within IAI46_01350 at 296.144 kb on - strand, within IAI46_01350 at 296.144 kb on - strand, within IAI46_01350 at 296.172 kb on + strand, within IAI46_01350 at 296.246 kb on - strand, within IAI46_01350 at 296.270 kb on - strand, within IAI46_01350 at 296.271 kb on - strand, within IAI46_01350 at 296.318 kb on + strand at 296.321 kb on + strand at 296.322 kb on - strand at 296.322 kb on - strand at 296.344 kb on - strand at 296.486 kb on - strand, within IAI46_01355 at 296.543 kb on + strand, within IAI46_01355 at 296.544 kb on - strand, within IAI46_01355 at 296.583 kb on - strand, within IAI46_01355 at 296.614 kb on + strand, within IAI46_01355 at 296.647 kb on + strand, within IAI46_01355 at 296.648 kb on - strand, within IAI46_01355 at 296.679 kb on + strand, within IAI46_01355 at 296.680 kb on - strand, within IAI46_01355 at 296.680 kb on - strand, within IAI46_01355 at 296.686 kb on + strand, within IAI46_01355 at 296.686 kb on + strand, within IAI46_01355 at 296.686 kb on + strand, within IAI46_01355 at 296.686 kb on + strand, within IAI46_01355 at 296.744 kb on + strand, within IAI46_01355 at 296.744 kb on + strand, within IAI46_01355 at 296.744 kb on + strand, within IAI46_01355 at 296.779 kb on + strand, within IAI46_01355 at 296.780 kb on - strand, within IAI46_01355 at 296.780 kb on - strand, within IAI46_01355 at 297.025 kb on - strand at 297.026 kb on + strand at 297.063 kb on + strand at 297.155 kb on + strand at 297.156 kb on + strand at 297.247 kb on + strand, within IAI46_01360 at 297.248 kb on - strand, within IAI46_01360 at 297.266 kb on + strand, within IAI46_01360 at 297.266 kb on + strand, within IAI46_01360 at 297.267 kb on - strand, within IAI46_01360 at 297.323 kb on - strand, within IAI46_01360 at 297.323 kb on - strand, within IAI46_01360 at 297.323 kb on - strand, within IAI46_01360 at 297.324 kb on + strand, within IAI46_01360 at 297.324 kb on + strand, within IAI46_01360 at 297.325 kb on - strand, within IAI46_01360 at 297.325 kb on - strand, within IAI46_01360 at 297.325 kb on - strand, within IAI46_01360 at 297.353 kb on + strand, within IAI46_01360 at 297.546 kb on + strand, within IAI46_01360 at 297.561 kb on + strand, within IAI46_01360 at 297.561 kb on + strand, within IAI46_01360 at 297.561 kb on + strand, within IAI46_01360 at 297.562 kb on - strand, within IAI46_01360 at 297.562 kb on - strand, within IAI46_01360 at 297.562 kb on - strand, within IAI46_01360 at 297.562 kb on - strand, within IAI46_01360 at 297.562 kb on - strand, within IAI46_01360 at 297.562 kb on - strand, within IAI46_01360 at 297.569 kb on - strand, within IAI46_01360 at 297.569 kb on - strand, within IAI46_01360 at 297.604 kb on + strand, within IAI46_01360 at 297.604 kb on + strand, within IAI46_01360 at 297.605 kb on - strand, within IAI46_01360 at 297.660 kb on + strand, within IAI46_01360 at 297.660 kb on + strand, within IAI46_01360 at 297.660 kb on + strand, within IAI46_01360 at 297.661 kb on - strand, within IAI46_01360 at 297.661 kb on - strand, within IAI46_01360 at 297.670 kb on + strand, within IAI46_01360 at 297.741 kb on - strand, within IAI46_01360 at 297.766 kb on + strand, within IAI46_01360 at 297.946 kb on + strand, within IAI46_01360 at 297.946 kb on + strand, within IAI46_01360 at 297.947 kb on - strand, within IAI46_01360 at 297.957 kb on + strand, within IAI46_01360 at 297.958 kb on - strand, within IAI46_01360 at 297.958 kb on - strand, within IAI46_01360 at 297.958 kb on - strand, within IAI46_01360 at 298.009 kb on + strand, within IAI46_01360 at 298.010 kb on - strand, within IAI46_01360 at 298.055 kb on + strand, within IAI46_01360 at 298.202 kb on - strand, within IAI46_01360 at 298.228 kb on + strand, within IAI46_01360 at 298.229 kb on - strand at 298.229 kb on - strand at 298.273 kb on - strand at 298.346 kb on + strand at 298.426 kb on + strand at 298.427 kb on - strand at 298.667 kb on + strand, within IAI46_01365 at 298.667 kb on + strand, within IAI46_01365 at 298.667 kb on + strand, within IAI46_01365 at 298.668 kb on - strand, within IAI46_01365 at 298.668 kb on - strand, within IAI46_01365 at 298.668 kb on - strand, within IAI46_01365 at 298.695 kb on + strand, within IAI46_01365 at 298.715 kb on - strand, within IAI46_01365 at 298.715 kb on - strand, within IAI46_01365 at 298.715 kb on - strand, within IAI46_01365 at 298.768 kb on + strand, within IAI46_01365 at 298.768 kb on + strand, within IAI46_01365 at 298.768 kb on + strand, within IAI46_01365 at 298.769 kb on - strand, within IAI46_01365 at 298.787 kb on + strand, within IAI46_01365 at 298.788 kb on - strand, within IAI46_01365 at 298.788 kb on - strand, within IAI46_01365 at 298.957 kb on + strand, within IAI46_01365 at 298.957 kb on + strand, within IAI46_01365 at 298.958 kb on - strand, within IAI46_01365 at 298.958 kb on - strand, within IAI46_01365 at 298.993 kb on + strand, within IAI46_01365 at 299.074 kb on - strand, within IAI46_01365 at 299.074 kb on - strand, within IAI46_01365 at 299.085 kb on + strand, within IAI46_01365 at 299.086 kb on - strand, within IAI46_01365 at 299.098 kb on + strand, within IAI46_01365 at 299.211 kb on + strand, within IAI46_01365 at 299.211 kb on + strand, within IAI46_01365 at 299.276 kb on + strand, within IAI46_01365 at 299.276 kb on + strand, within IAI46_01365 at 299.276 kb on + strand, within IAI46_01365 at 299.277 kb on - strand, within IAI46_01365 at 299.277 kb on - strand, within IAI46_01365 at 299.277 kb on - strand, within IAI46_01365 at 299.277 kb on - strand, within IAI46_01365 at 299.279 kb on - strand, within IAI46_01365 at 299.279 kb on - strand, within IAI46_01365 at 299.280 kb on + strand, within IAI46_01365 at 299.280 kb on + strand, within IAI46_01365 at 299.280 kb on + strand, within IAI46_01365 at 299.280 kb on + strand, within IAI46_01365 at 299.281 kb on - strand, within IAI46_01365 at 299.281 kb on - strand, within IAI46_01365 at 299.281 kb on - strand, within IAI46_01365 at 299.281 kb on - strand, within IAI46_01365 at 299.281 kb on - strand, within IAI46_01365 at 299.313 kb on + strand, within IAI46_01365 at 299.337 kb on + strand, within IAI46_01365 at 299.337 kb on + strand, within IAI46_01365 at 299.337 kb on + strand, within IAI46_01365 at 299.337 kb on + strand, within IAI46_01365 at 299.337 kb on + strand, within IAI46_01365 at 299.337 kb on + strand, within IAI46_01365 at 299.338 kb on - strand, within IAI46_01365 at 299.338 kb on - strand, within IAI46_01365 at 299.338 kb on - strand, within IAI46_01365 at 299.341 kb on + strand, within IAI46_01365 at 299.341 kb on + strand, within IAI46_01365 at 299.341 kb on + strand, within IAI46_01365 at 299.341 kb on + strand, within IAI46_01365 at 299.342 kb on - strand, within IAI46_01365
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 296,110 + IAI46_01350 0.23 +0.3 296,140 + IAI46_01350 0.34 -0.8 296,143 + IAI46_01350 0.35 -0.2 296,144 - IAI46_01350 0.35 -2.1 296,144 - IAI46_01350 0.35 -1.0 296,144 - IAI46_01350 0.35 -0.3 296,172 + IAI46_01350 0.45 -1.6 296,246 - IAI46_01350 0.73 -0.0 296,270 - IAI46_01350 0.81 -0.7 296,271 - IAI46_01350 0.82 -0.3 296,318 + -0.4 296,321 + -0.8 296,322 - +0.1 296,322 - -0.1 296,344 - +0.4 296,486 - IAI46_01355 0.15 -0.4 296,543 + IAI46_01355 0.24 +1.0 296,544 - IAI46_01355 0.24 +0.0 296,583 - IAI46_01355 0.30 -0.0 296,614 + IAI46_01355 0.35 +0.8 296,647 + IAI46_01355 0.40 -0.0 296,648 - IAI46_01355 0.40 -1.7 296,679 + IAI46_01355 0.45 +0.1 296,680 - IAI46_01355 0.45 -0.0 296,680 - IAI46_01355 0.45 -0.1 296,686 + IAI46_01355 0.46 +0.8 296,686 + IAI46_01355 0.46 -1.5 296,686 + IAI46_01355 0.46 +1.0 296,686 + IAI46_01355 0.46 -1.0 296,744 + IAI46_01355 0.55 +0.3 296,744 + IAI46_01355 0.55 +0.0 296,744 + IAI46_01355 0.55 -0.6 296,779 + IAI46_01355 0.61 +0.4 296,780 - IAI46_01355 0.61 +0.3 296,780 - IAI46_01355 0.61 -0.7 297,025 - -0.3 297,026 + +0.7 297,063 + +0.3 297,155 + -1.3 297,156 + -0.9 297,247 + IAI46_01360 0.13 -1.0 297,248 - IAI46_01360 0.14 -1.0 297,266 + IAI46_01360 0.15 +1.0 297,266 + IAI46_01360 0.15 -1.3 297,267 - IAI46_01360 0.15 -1.1 297,323 - IAI46_01360 0.19 -3.1 297,323 - IAI46_01360 0.19 -1.5 297,323 - IAI46_01360 0.19 -1.2 297,324 + IAI46_01360 0.19 -0.9 297,324 + IAI46_01360 0.19 -3.2 297,325 - IAI46_01360 0.20 -1.6 297,325 - IAI46_01360 0.20 -1.9 297,325 - IAI46_01360 0.20 -0.5 297,353 + IAI46_01360 0.22 -1.5 297,546 + IAI46_01360 0.37 -2.9 297,561 + IAI46_01360 0.38 -0.9 297,561 + IAI46_01360 0.38 -0.3 297,561 + IAI46_01360 0.38 -0.7 297,562 - IAI46_01360 0.38 -1.5 297,562 - IAI46_01360 0.38 -1.2 297,562 - IAI46_01360 0.38 -1.2 297,562 - IAI46_01360 0.38 -3.6 297,562 - IAI46_01360 0.38 -2.2 297,562 - IAI46_01360 0.38 -1.5 297,569 - IAI46_01360 0.39 -1.6 297,569 - IAI46_01360 0.39 -1.4 297,604 + IAI46_01360 0.41 -0.9 297,604 + IAI46_01360 0.41 -1.0 297,605 - IAI46_01360 0.41 -2.6 297,660 + IAI46_01360 0.46 -1.1 297,660 + IAI46_01360 0.46 -1.7 297,660 + IAI46_01360 0.46 -0.9 297,661 - IAI46_01360 0.46 -1.0 297,661 - IAI46_01360 0.46 -1.0 297,670 + IAI46_01360 0.46 -0.3 297,741 - IAI46_01360 0.52 -1.7 297,766 + IAI46_01360 0.54 -0.7 297,946 + IAI46_01360 0.68 -0.0 297,946 + IAI46_01360 0.68 -2.2 297,947 - IAI46_01360 0.68 -0.4 297,957 + IAI46_01360 0.69 -0.4 297,958 - IAI46_01360 0.69 -1.3 297,958 - IAI46_01360 0.69 -1.2 297,958 - IAI46_01360 0.69 -2.5 298,009 + IAI46_01360 0.73 -1.5 298,010 - IAI46_01360 0.73 -1.2 298,055 + IAI46_01360 0.76 -0.6 298,202 - IAI46_01360 0.88 +0.4 298,228 + IAI46_01360 0.90 -0.0 298,229 - -1.7 298,229 - -2.2 298,273 - -2.0 298,346 + -1.6 298,426 + -0.9 298,427 - -3.0 298,667 + IAI46_01365 0.16 -1.5 298,667 + IAI46_01365 0.16 -1.0 298,667 + IAI46_01365 0.16 -1.8 298,668 - IAI46_01365 0.16 -2.2 298,668 - IAI46_01365 0.16 +1.8 298,668 - IAI46_01365 0.16 -1.8 298,695 + IAI46_01365 0.18 -1.2 298,715 - IAI46_01365 0.19 -0.0 298,715 - IAI46_01365 0.19 -2.3 298,715 - IAI46_01365 0.19 -1.6 298,768 + IAI46_01365 0.23 -2.5 298,768 + IAI46_01365 0.23 +0.2 298,768 + IAI46_01365 0.23 -2.1 298,769 - IAI46_01365 0.23 -3.3 298,787 + IAI46_01365 0.24 -1.7 298,788 - IAI46_01365 0.24 -2.2 298,788 - IAI46_01365 0.24 -3.3 298,957 + IAI46_01365 0.35 +0.8 298,957 + IAI46_01365 0.35 -3.0 298,958 - IAI46_01365 0.35 -1.9 298,958 - IAI46_01365 0.35 -4.1 298,993 + IAI46_01365 0.37 +1.7 299,074 - IAI46_01365 0.42 -0.3 299,074 - IAI46_01365 0.42 -1.9 299,085 + IAI46_01365 0.43 -1.8 299,086 - IAI46_01365 0.43 -1.4 299,098 + IAI46_01365 0.44 -1.0 299,211 + IAI46_01365 0.51 -1.5 299,211 + IAI46_01365 0.51 -2.8 299,276 + IAI46_01365 0.55 -1.1 299,276 + IAI46_01365 0.55 -1.5 299,276 + IAI46_01365 0.55 -2.4 299,277 - IAI46_01365 0.55 -2.7 299,277 - IAI46_01365 0.55 +0.4 299,277 - IAI46_01365 0.55 -2.2 299,277 - IAI46_01365 0.55 -1.5 299,279 - IAI46_01365 0.55 -1.2 299,279 - IAI46_01365 0.55 -1.2 299,280 + IAI46_01365 0.55 -1.1 299,280 + IAI46_01365 0.55 -0.6 299,280 + IAI46_01365 0.55 -0.0 299,280 + IAI46_01365 0.55 -2.6 299,281 - IAI46_01365 0.55 -0.6 299,281 - IAI46_01365 0.55 -2.1 299,281 - IAI46_01365 0.55 -0.9 299,281 - IAI46_01365 0.55 -1.3 299,281 - IAI46_01365 0.55 -1.2 299,313 + IAI46_01365 0.57 -1.7 299,337 + IAI46_01365 0.59 -0.9 299,337 + IAI46_01365 0.59 -1.5 299,337 + IAI46_01365 0.59 -0.9 299,337 + IAI46_01365 0.59 -2.4 299,337 + IAI46_01365 0.59 +0.5 299,337 + IAI46_01365 0.59 -0.9 299,338 - IAI46_01365 0.59 -1.8 299,338 - IAI46_01365 0.59 -0.3 299,338 - IAI46_01365 0.59 -1.5 299,341 + IAI46_01365 0.59 -1.5 299,341 + IAI46_01365 0.59 -1.2 299,341 + IAI46_01365 0.59 -3.6 299,341 + IAI46_01365 0.59 -1.4 299,342 - IAI46_01365 0.59 -1.7
Or see this region's nucleotide sequence