Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS28505 and ABIE40_RS28510 are separated by 137 nucleotides ABIE40_RS28510 and ABIE40_RS28515 overlap by 4 nucleotides ABIE40_RS28515 and ABIE40_RS28520 overlap by 52 nucleotides
ABIE40_RS28505: ABIE40_RS28505 - ABATE domain-containing protein, at 403,485 to 404,099
_RS28505
ABIE40_RS28510: ABIE40_RS28510 - SMP-30/gluconolactonase/LRE family protein, at 404,237 to 404,995
_RS28510
ABIE40_RS28515: ABIE40_RS28515 - hypothetical protein, at 404,992 to 405,351
_RS28515
ABIE40_RS28520: ABIE40_RS28520 - glycoside hydrolase family 15 protein, at 405,300 to 406,778
_RS28520
Position (kb)
404
405
406 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 404.097 kb on + strand at 404.148 kb on + strand at 404.174 kb on + strand at 404.175 kb on - strand at 404.175 kb on - strand at 404.181 kb on - strand at 404.184 kb on + strand at 404.184 kb on + strand at 404.212 kb on - strand at 404.314 kb on + strand, within ABIE40_RS28510 at 404.343 kb on + strand, within ABIE40_RS28510 at 404.344 kb on - strand, within ABIE40_RS28510 at 404.344 kb on - strand, within ABIE40_RS28510 at 404.344 kb on - strand, within ABIE40_RS28510 at 404.381 kb on + strand, within ABIE40_RS28510 at 404.441 kb on + strand, within ABIE40_RS28510 at 404.441 kb on + strand, within ABIE40_RS28510 at 404.478 kb on + strand, within ABIE40_RS28510 at 404.478 kb on + strand, within ABIE40_RS28510 at 404.479 kb on - strand, within ABIE40_RS28510 at 404.479 kb on - strand, within ABIE40_RS28510 at 404.479 kb on - strand, within ABIE40_RS28510 at 404.501 kb on - strand, within ABIE40_RS28510 at 404.520 kb on + strand, within ABIE40_RS28510 at 404.520 kb on + strand, within ABIE40_RS28510 at 404.521 kb on - strand, within ABIE40_RS28510 at 404.521 kb on - strand, within ABIE40_RS28510 at 404.524 kb on + strand, within ABIE40_RS28510 at 404.524 kb on + strand, within ABIE40_RS28510 at 404.525 kb on - strand, within ABIE40_RS28510 at 404.527 kb on - strand, within ABIE40_RS28510 at 404.610 kb on + strand, within ABIE40_RS28510 at 404.611 kb on - strand, within ABIE40_RS28510 at 404.735 kb on + strand, within ABIE40_RS28510 at 404.761 kb on - strand, within ABIE40_RS28510 at 404.982 kb on - strand at 405.019 kb on + strand at 405.185 kb on + strand, within ABIE40_RS28515 at 405.186 kb on - strand, within ABIE40_RS28515 at 405.186 kb on - strand, within ABIE40_RS28515 at 405.186 kb on - strand, within ABIE40_RS28515 at 405.186 kb on - strand, within ABIE40_RS28515 at 405.186 kb on - strand, within ABIE40_RS28515 at 405.249 kb on - strand, within ABIE40_RS28515 at 405.255 kb on - strand, within ABIE40_RS28515 at 405.255 kb on - strand, within ABIE40_RS28515 at 405.380 kb on + strand at 405.381 kb on - strand at 405.381 kb on - strand at 405.437 kb on + strand at 405.504 kb on + strand, within ABIE40_RS28520 at 405.550 kb on + strand, within ABIE40_RS28520 at 405.550 kb on + strand, within ABIE40_RS28520 at 405.550 kb on + strand, within ABIE40_RS28520 at 405.551 kb on - strand, within ABIE40_RS28520 at 405.551 kb on - strand, within ABIE40_RS28520 at 405.770 kb on - strand, within ABIE40_RS28520 at 405.823 kb on + strand, within ABIE40_RS28520 at 405.823 kb on + strand, within ABIE40_RS28520 at 405.824 kb on - strand, within ABIE40_RS28520 at 405.824 kb on - strand, within ABIE40_RS28520 at 405.931 kb on + strand, within ABIE40_RS28520 at 405.932 kb on - strand, within ABIE40_RS28520 at 405.932 kb on - strand, within ABIE40_RS28520 at 405.932 kb on - strand, within ABIE40_RS28520 at 405.961 kb on + strand, within ABIE40_RS28520 at 405.962 kb on - strand, within ABIE40_RS28520 at 405.967 kb on + strand, within ABIE40_RS28520 at 406.052 kb on + strand, within ABIE40_RS28520 at 406.103 kb on + strand, within ABIE40_RS28520 at 406.103 kb on + strand, within ABIE40_RS28520 at 406.103 kb on + strand, within ABIE40_RS28520 at 406.104 kb on - strand, within ABIE40_RS28520 at 406.104 kb on - strand, within ABIE40_RS28520 at 406.104 kb on - strand, within ABIE40_RS28520 at 406.243 kb on + strand, within ABIE40_RS28520 at 406.243 kb on + strand, within ABIE40_RS28520 at 406.328 kb on - strand, within ABIE40_RS28520 at 406.328 kb on - strand, within ABIE40_RS28520
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 404,097 + -1.8 404,148 + -1.7 404,174 + -0.8 404,175 - +2.5 404,175 - +0.4 404,181 - -0.8 404,184 + +1.8 404,184 + -2.4 404,212 - -1.7 404,314 + ABIE40_RS28510 0.10 +2.1 404,343 + ABIE40_RS28510 0.14 -1.2 404,344 - ABIE40_RS28510 0.14 -3.7 404,344 - ABIE40_RS28510 0.14 +0.1 404,344 - ABIE40_RS28510 0.14 -0.8 404,381 + ABIE40_RS28510 0.19 +0.2 404,441 + ABIE40_RS28510 0.27 -1.2 404,441 + ABIE40_RS28510 0.27 +0.1 404,478 + ABIE40_RS28510 0.32 -0.0 404,478 + ABIE40_RS28510 0.32 -1.6 404,479 - ABIE40_RS28510 0.32 -0.0 404,479 - ABIE40_RS28510 0.32 +0.2 404,479 - ABIE40_RS28510 0.32 +0.4 404,501 - ABIE40_RS28510 0.35 -0.7 404,520 + ABIE40_RS28510 0.37 +0.2 404,520 + ABIE40_RS28510 0.37 -1.8 404,521 - ABIE40_RS28510 0.37 -0.1 404,521 - ABIE40_RS28510 0.37 +0.4 404,524 + ABIE40_RS28510 0.38 -0.2 404,524 + ABIE40_RS28510 0.38 +0.5 404,525 - ABIE40_RS28510 0.38 -0.7 404,527 - ABIE40_RS28510 0.38 -0.4 404,610 + ABIE40_RS28510 0.49 -3.1 404,611 - ABIE40_RS28510 0.49 +0.1 404,735 + ABIE40_RS28510 0.66 -1.6 404,761 - ABIE40_RS28510 0.69 -3.1 404,982 - -0.9 405,019 + +0.4 405,185 + ABIE40_RS28515 0.54 +0.4 405,186 - ABIE40_RS28515 0.54 +1.5 405,186 - ABIE40_RS28515 0.54 +0.5 405,186 - ABIE40_RS28515 0.54 -1.1 405,186 - ABIE40_RS28515 0.54 -0.4 405,186 - ABIE40_RS28515 0.54 -0.3 405,249 - ABIE40_RS28515 0.71 +0.5 405,255 - ABIE40_RS28515 0.73 -2.1 405,255 - ABIE40_RS28515 0.73 -1.2 405,380 + +0.4 405,381 - -0.9 405,381 - -0.5 405,437 + -1.2 405,504 + ABIE40_RS28520 0.14 -3.3 405,550 + ABIE40_RS28520 0.17 +0.7 405,550 + ABIE40_RS28520 0.17 -3.0 405,550 + ABIE40_RS28520 0.17 +1.2 405,551 - ABIE40_RS28520 0.17 -2.3 405,551 - ABIE40_RS28520 0.17 -2.5 405,770 - ABIE40_RS28520 0.32 -1.2 405,823 + ABIE40_RS28520 0.35 -2.9 405,823 + ABIE40_RS28520 0.35 -2.9 405,824 - ABIE40_RS28520 0.35 +1.3 405,824 - ABIE40_RS28520 0.35 -0.6 405,931 + ABIE40_RS28520 0.43 -4.5 405,932 - ABIE40_RS28520 0.43 -2.6 405,932 - ABIE40_RS28520 0.43 -1.8 405,932 - ABIE40_RS28520 0.43 +0.5 405,961 + ABIE40_RS28520 0.45 +0.1 405,962 - ABIE40_RS28520 0.45 -0.9 405,967 + ABIE40_RS28520 0.45 -1.1 406,052 + ABIE40_RS28520 0.51 +0.0 406,103 + ABIE40_RS28520 0.54 -0.8 406,103 + ABIE40_RS28520 0.54 -0.3 406,103 + ABIE40_RS28520 0.54 +0.8 406,104 - ABIE40_RS28520 0.54 +2.6 406,104 - ABIE40_RS28520 0.54 +1.8 406,104 - ABIE40_RS28520 0.54 -0.4 406,243 + ABIE40_RS28520 0.64 +0.1 406,243 + ABIE40_RS28520 0.64 -1.1 406,328 - ABIE40_RS28520 0.70 +0.7 406,328 - ABIE40_RS28520 0.70 -4.0
Or see this region's nucleotide sequence