Strain Fitness in Escherichia coli BW25113 around b1816

Experiment: D-Mannose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1815 and yoaE are separated by 3 nucleotidesyoaE and manX are separated by 462 nucleotides b1815: b1815 - orf, hypothetical protein (VIMSS), at 1,896,451 to 1,898,049 b1815 b1816: yoaE - fused predicted membrane protein/conserved protein (NCBI), at 1,898,053 to 1,899,609 yoaE b1817: manX - fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI), at 1,900,072 to 1,901,043 manX Position (kb) 1898 1899 1900Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1897.151 kb on - strand, within b1815at 1897.353 kb on - strand, within b1815at 1897.505 kb on - strand, within b1815at 1897.505 kb on - strand, within b1815at 1897.514 kb on - strand, within b1815at 1897.514 kb on - strand, within b1815at 1897.543 kb on - strand, within b1815at 1897.597 kb on + strand, within b1815at 1898.074 kb on + strandat 1898.454 kb on - strand, within yoaEat 1898.667 kb on + strand, within yoaEat 1898.794 kb on - strand, within yoaEat 1898.843 kb on + strand, within yoaEat 1898.864 kb on + strand, within yoaEat 1898.914 kb on - strand, within yoaEat 1898.925 kb on + strand, within yoaEat 1898.932 kb on - strand, within yoaEat 1898.932 kb on - strand, within yoaEat 1899.020 kb on - strand, within yoaEat 1899.020 kb on - strand, within yoaEat 1899.103 kb on + strand, within yoaEat 1899.103 kb on + strand, within yoaEat 1899.429 kb on + strand, within yoaEat 1899.689 kb on + strandat 1899.769 kb on + strandat 1899.804 kb on + strandat 1899.991 kb on - strandat 1900.072 kb on - strandat 1900.112 kb on - strandat 1900.117 kb on - strandat 1900.225 kb on + strand, within manXat 1900.253 kb on - strand, within manXat 1900.413 kb on + strand, within manX

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Mannose (C)
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1,897,151 - b1815 b1815 0.44 -0.9
1,897,353 - b1815 b1815 0.56 +0.0
1,897,505 - b1815 b1815 0.66 -0.3
1,897,505 - b1815 b1815 0.66 +0.4
1,897,514 - b1815 b1815 0.66 -0.1
1,897,514 - b1815 b1815 0.66 -2.1
1,897,543 - b1815 b1815 0.68 -0.6
1,897,597 + b1815 b1815 0.72 +1.4
1,898,074 + +0.9
1,898,454 - yoaE b1816 0.26 +0.2
1,898,667 + yoaE b1816 0.39 -0.2
1,898,794 - yoaE b1816 0.48 +0.0
1,898,843 + yoaE b1816 0.51 +0.1
1,898,864 + yoaE b1816 0.52 +0.5
1,898,914 - yoaE b1816 0.55 +0.0
1,898,925 + yoaE b1816 0.56 +0.3
1,898,932 - yoaE b1816 0.56 -0.2
1,898,932 - yoaE b1816 0.56 -0.1
1,899,020 - yoaE b1816 0.62 +0.3
1,899,020 - yoaE b1816 0.62 +1.0
1,899,103 + yoaE b1816 0.67 +2.2
1,899,103 + yoaE b1816 0.67 -0.2
1,899,429 + yoaE b1816 0.88 +0.0
1,899,689 + +0.8
1,899,769 + -0.3
1,899,804 + +0.8
1,899,991 - -0.8
1,900,072 - -3.6
1,900,112 - -2.1
1,900,117 - -2.7
1,900,225 + manX b1817 0.16 -1.7
1,900,253 - manX b1817 0.19 -2.1
1,900,413 + manX b1817 0.35 -1.5

Or see this region's nucleotide sequence