Experiment: Manganese (II) chloride tetrahydrate 50 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_15335 and IAI46_15340 are separated by 740 nucleotides IAI46_15340 and IAI46_15345 are separated by 61 nucleotides
IAI46_15335: IAI46_15335 - aspartate aminotransferase family protein, at 3,209,351 to 3,210,565
_15335
IAI46_15340: IAI46_15340 - extracellular solute-binding protein, at 3,211,306 to 3,212,580
_15340
IAI46_15345: IAI46_15345 - sugar ABC transporter permease, at 3,212,642 to 3,213,583
_15345
IAI46_15350: IAI46_15350 - carbohydrate ABC transporter permease, at 3,213,573 to 3,214,406
_15350
Position (kb)
3211
3212
3213 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3210.438 kb on + strand, within IAI46_15335 at 3210.439 kb on - strand, within IAI46_15335 at 3210.439 kb on - strand, within IAI46_15335 at 3210.544 kb on + strand at 3210.577 kb on - strand at 3210.577 kb on - strand at 3210.605 kb on + strand at 3210.606 kb on - strand at 3210.682 kb on + strand at 3210.683 kb on - strand at 3210.722 kb on - strand at 3210.746 kb on + strand at 3210.746 kb on + strand at 3210.746 kb on + strand at 3210.746 kb on + strand at 3210.747 kb on - strand at 3210.747 kb on - strand at 3210.747 kb on - strand at 3210.747 kb on - strand at 3210.747 kb on - strand at 3210.747 kb on - strand at 3210.747 kb on - strand at 3210.767 kb on + strand at 3210.824 kb on - strand at 3211.110 kb on - strand at 3211.180 kb on + strand at 3211.180 kb on + strand at 3211.181 kb on - strand at 3211.188 kb on - strand at 3211.226 kb on + strand at 3211.230 kb on - strand at 3211.302 kb on + strand at 3211.330 kb on - strand at 3211.332 kb on + strand at 3211.333 kb on - strand at 3211.417 kb on + strand at 3211.418 kb on - strand at 3211.418 kb on - strand at 3211.432 kb on + strand at 3211.621 kb on - strand, within IAI46_15340 at 3211.650 kb on + strand, within IAI46_15340 at 3211.710 kb on + strand, within IAI46_15340 at 3211.711 kb on - strand, within IAI46_15340 at 3211.711 kb on - strand, within IAI46_15340 at 3211.711 kb on - strand, within IAI46_15340 at 3211.711 kb on - strand, within IAI46_15340 at 3211.711 kb on - strand, within IAI46_15340 at 3211.714 kb on + strand, within IAI46_15340 at 3211.714 kb on + strand, within IAI46_15340 at 3211.714 kb on + strand, within IAI46_15340 at 3211.714 kb on + strand, within IAI46_15340 at 3211.715 kb on - strand, within IAI46_15340 at 3211.715 kb on - strand, within IAI46_15340 at 3211.753 kb on + strand, within IAI46_15340 at 3211.758 kb on + strand, within IAI46_15340 at 3211.759 kb on - strand, within IAI46_15340 at 3211.784 kb on + strand, within IAI46_15340 at 3211.785 kb on - strand, within IAI46_15340 at 3211.937 kb on - strand, within IAI46_15340 at 3211.995 kb on - strand, within IAI46_15340 at 3212.070 kb on - strand, within IAI46_15340 at 3212.275 kb on + strand, within IAI46_15340 at 3212.347 kb on + strand, within IAI46_15340 at 3212.359 kb on + strand, within IAI46_15340 at 3212.359 kb on + strand, within IAI46_15340 at 3212.359 kb on + strand, within IAI46_15340 at 3212.464 kb on - strand at 3212.548 kb on - strand at 3212.647 kb on + strand at 3212.647 kb on + strand at 3212.832 kb on - strand, within IAI46_15345 at 3212.834 kb on + strand, within IAI46_15345 at 3212.881 kb on + strand, within IAI46_15345 at 3213.000 kb on - strand, within IAI46_15345 at 3213.203 kb on - strand, within IAI46_15345 at 3213.203 kb on - strand, within IAI46_15345 at 3213.203 kb on - strand, within IAI46_15345 at 3213.213 kb on - strand, within IAI46_15345 at 3213.249 kb on - strand, within IAI46_15345 at 3213.298 kb on + strand, within IAI46_15345 at 3213.326 kb on - strand, within IAI46_15345 at 3213.441 kb on - strand, within IAI46_15345 at 3213.447 kb on - strand, within IAI46_15345 at 3213.447 kb on - strand, within IAI46_15345 at 3213.465 kb on - strand, within IAI46_15345 at 3213.473 kb on - strand, within IAI46_15345
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 50 uM, aerobic remove 3,210,438 + IAI46_15335 0.89 +1.0 3,210,439 - IAI46_15335 0.90 +0.5 3,210,439 - IAI46_15335 0.90 +0.6 3,210,544 + +0.3 3,210,577 - -0.7 3,210,577 - +0.3 3,210,605 + -0.7 3,210,606 - +0.4 3,210,682 + +0.1 3,210,683 - +0.1 3,210,722 - +1.3 3,210,746 + +0.0 3,210,746 + +0.4 3,210,746 + +0.9 3,210,746 + +1.0 3,210,747 - -1.1 3,210,747 - +0.5 3,210,747 - +0.6 3,210,747 - -0.1 3,210,747 - +1.2 3,210,747 - +0.5 3,210,747 - -0.2 3,210,767 + +0.4 3,210,824 - +0.8 3,211,110 - +0.5 3,211,180 + +1.0 3,211,180 + +0.4 3,211,181 - -0.3 3,211,188 - -0.3 3,211,226 + +0.6 3,211,230 - +0.4 3,211,302 + -0.4 3,211,330 - -0.8 3,211,332 + +0.2 3,211,333 - +1.9 3,211,417 + -1.9 3,211,418 - +0.5 3,211,418 - -0.6 3,211,432 + +0.2 3,211,621 - IAI46_15340 0.25 +0.2 3,211,650 + IAI46_15340 0.27 +0.5 3,211,710 + IAI46_15340 0.32 -1.3 3,211,711 - IAI46_15340 0.32 +0.2 3,211,711 - IAI46_15340 0.32 -0.9 3,211,711 - IAI46_15340 0.32 +0.5 3,211,711 - IAI46_15340 0.32 -1.2 3,211,711 - IAI46_15340 0.32 +0.4 3,211,714 + IAI46_15340 0.32 +0.5 3,211,714 + IAI46_15340 0.32 +0.1 3,211,714 + IAI46_15340 0.32 +0.6 3,211,714 + IAI46_15340 0.32 +0.3 3,211,715 - IAI46_15340 0.32 -0.9 3,211,715 - IAI46_15340 0.32 +0.2 3,211,753 + IAI46_15340 0.35 +0.5 3,211,758 + IAI46_15340 0.35 +0.4 3,211,759 - IAI46_15340 0.36 +0.4 3,211,784 + IAI46_15340 0.37 +0.4 3,211,785 - IAI46_15340 0.38 -0.9 3,211,937 - IAI46_15340 0.49 +0.2 3,211,995 - IAI46_15340 0.54 +0.6 3,212,070 - IAI46_15340 0.60 +0.6 3,212,275 + IAI46_15340 0.76 +0.2 3,212,347 + IAI46_15340 0.82 -0.9 3,212,359 + IAI46_15340 0.83 -0.2 3,212,359 + IAI46_15340 0.83 +0.2 3,212,359 + IAI46_15340 0.83 -1.2 3,212,464 - +0.0 3,212,548 - -0.8 3,212,647 + +1.4 3,212,647 + +0.6 3,212,832 - IAI46_15345 0.20 -0.4 3,212,834 + IAI46_15345 0.20 -0.1 3,212,881 + IAI46_15345 0.25 -3.2 3,213,000 - IAI46_15345 0.38 +0.5 3,213,203 - IAI46_15345 0.60 -0.7 3,213,203 - IAI46_15345 0.60 -0.0 3,213,203 - IAI46_15345 0.60 -0.4 3,213,213 - IAI46_15345 0.61 -0.4 3,213,249 - IAI46_15345 0.64 +1.4 3,213,298 + IAI46_15345 0.70 -0.2 3,213,326 - IAI46_15345 0.73 -0.6 3,213,441 - IAI46_15345 0.85 +0.6 3,213,447 - IAI46_15345 0.85 -0.9 3,213,447 - IAI46_15345 0.85 -0.3 3,213,465 - IAI46_15345 0.87 -0.1 3,213,473 - IAI46_15345 0.88 -0.3
Or see this region's nucleotide sequence