Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12315 and AMB_RS12320 are separated by 18 nucleotides AMB_RS12320 and AMB_RS12325 are separated by 40 nucleotides
AMB_RS12315: AMB_RS12315 - D-alanyl-D-alanine carboxypeptidase, at 2,627,277 to 2,628,440
_RS12315
AMB_RS12320: AMB_RS12320 - septal ring lytic transglycosylase RlpA family protein, at 2,628,459 to 2,629,433
_RS12320
AMB_RS12325: AMB_RS12325 - lytic murein transglycosylase, at 2,629,474 to 2,630,544
_RS12325
Position (kb)
2628
2629
2630 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2627.459 kb on - strand, within AMB_RS12315 at 2627.459 kb on - strand, within AMB_RS12315 at 2627.459 kb on - strand, within AMB_RS12315 at 2627.606 kb on - strand, within AMB_RS12315 at 2627.606 kb on - strand, within AMB_RS12315 at 2627.606 kb on - strand, within AMB_RS12315 at 2627.606 kb on - strand, within AMB_RS12315 at 2628.320 kb on - strand, within AMB_RS12315 at 2628.461 kb on - strand at 2628.677 kb on - strand, within AMB_RS12320 at 2628.677 kb on - strand, within AMB_RS12320 at 2629.286 kb on - strand, within AMB_RS12320 at 2629.286 kb on - strand, within AMB_RS12320 at 2629.476 kb on - strand at 2629.565 kb on + strand at 2629.565 kb on + strand at 2629.565 kb on + strand at 2629.788 kb on - strand, within AMB_RS12325 at 2629.869 kb on + strand, within AMB_RS12325 at 2629.870 kb on - strand, within AMB_RS12325 at 2629.945 kb on - strand, within AMB_RS12325 at 2630.120 kb on + strand, within AMB_RS12325 at 2630.213 kb on + strand, within AMB_RS12325
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 2,627,459 - AMB_RS12315 0.16 +0.1 2,627,459 - AMB_RS12315 0.16 +1.3 2,627,459 - AMB_RS12315 0.16 +1.2 2,627,606 - AMB_RS12315 0.28 +0.1 2,627,606 - AMB_RS12315 0.28 -2.2 2,627,606 - AMB_RS12315 0.28 -1.6 2,627,606 - AMB_RS12315 0.28 -3.2 2,628,320 - AMB_RS12315 0.90 -2.4 2,628,461 - -1.3 2,628,677 - AMB_RS12320 0.22 -2.6 2,628,677 - AMB_RS12320 0.22 -0.8 2,629,286 - AMB_RS12320 0.85 -0.8 2,629,286 - AMB_RS12320 0.85 -2.3 2,629,476 - -0.0 2,629,565 + +0.1 2,629,565 + -1.0 2,629,565 + -0.7 2,629,788 - AMB_RS12325 0.29 -1.6 2,629,869 + AMB_RS12325 0.37 -0.7 2,629,870 - AMB_RS12325 0.37 +1.0 2,629,945 - AMB_RS12325 0.44 -2.3 2,630,120 + AMB_RS12325 0.60 -4.7 2,630,213 + AMB_RS12325 0.69 +0.8
Or see this region's nucleotide sequence