Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11595 and AMB_RS11600 are separated by 86 nucleotides AMB_RS11600 and AMB_RS11605 are separated by 146 nucleotides AMB_RS11605 and AMB_RS11610 are separated by 55 nucleotides AMB_RS11610 and AMB_RS11615 are separated by 60 nucleotides AMB_RS11615 and AMB_RS11620 are separated by 63 nucleotides
AMB_RS11595: AMB_RS11595 - PAS domain S-box protein, at 2,496,319 to 2,499,909
_RS11595
AMB_RS11600: AMB_RS11600 - 50S ribosomal protein L36, at 2,499,996 to 2,500,121
_RS11600
AMB_RS11605: AMB_RS11605 - N-formylglutamate amidohydrolase, at 2,500,268 to 2,501,059
_RS11605
AMB_RS11610: AMB_RS11610 - DUF2312 domain-containing protein, at 2,501,115 to 2,501,378
_RS11610
AMB_RS11615: AMB_RS11615 - diguanylate cyclase, at 2,501,439 to 2,501,849
_RS11615
AMB_RS11620: AMB_RS11620 - hypothetical protein, at 2,501,913 to 2,502,446
_RS11620
Position (kb)
2500
2501
2502 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2499.292 kb on + strand, within AMB_RS11595 at 2499.292 kb on + strand, within AMB_RS11595 at 2499.548 kb on + strand, within AMB_RS11595 at 2499.548 kb on + strand, within AMB_RS11595 at 2499.548 kb on + strand, within AMB_RS11595 at 2499.548 kb on + strand, within AMB_RS11595 at 2499.549 kb on - strand, within AMB_RS11595 at 2499.549 kb on - strand, within AMB_RS11595 at 2499.549 kb on - strand, within AMB_RS11595 at 2499.853 kb on + strand at 2499.964 kb on + strand at 2500.135 kb on - strand at 2500.255 kb on + strand at 2500.255 kb on + strand at 2500.255 kb on + strand at 2500.256 kb on - strand at 2500.256 kb on - strand at 2500.722 kb on + strand, within AMB_RS11605 at 2500.723 kb on - strand, within AMB_RS11605 at 2500.723 kb on - strand, within AMB_RS11605 at 2500.869 kb on - strand, within AMB_RS11605 at 2500.889 kb on + strand, within AMB_RS11605 at 2500.889 kb on + strand, within AMB_RS11605 at 2500.889 kb on + strand, within AMB_RS11605 at 2500.889 kb on + strand, within AMB_RS11605 at 2500.890 kb on - strand, within AMB_RS11605 at 2500.890 kb on - strand, within AMB_RS11605 at 2501.054 kb on + strand at 2501.054 kb on + strand at 2501.055 kb on - strand at 2501.079 kb on + strand at 2501.376 kb on + strand at 2501.377 kb on - strand at 2501.377 kb on - strand at 2501.391 kb on + strand at 2501.391 kb on + strand at 2501.392 kb on - strand at 2501.392 kb on - strand at 2501.440 kb on + strand at 2501.440 kb on + strand at 2501.441 kb on - strand at 2501.885 kb on - strand at 2501.885 kb on - strand at 2501.885 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 2,499,292 + AMB_RS11595 0.83 -1.0 2,499,292 + AMB_RS11595 0.83 -2.2 2,499,548 + AMB_RS11595 0.90 -4.4 2,499,548 + AMB_RS11595 0.90 -2.8 2,499,548 + AMB_RS11595 0.90 +0.0 2,499,548 + AMB_RS11595 0.90 +0.0 2,499,549 - AMB_RS11595 0.90 +1.0 2,499,549 - AMB_RS11595 0.90 -2.9 2,499,549 - AMB_RS11595 0.90 -0.2 2,499,853 + +0.0 2,499,964 + +0.9 2,500,135 - -1.4 2,500,255 + +0.9 2,500,255 + -0.6 2,500,255 + -2.4 2,500,256 - -1.5 2,500,256 - -2.9 2,500,722 + AMB_RS11605 0.57 -0.4 2,500,723 - AMB_RS11605 0.57 -1.9 2,500,723 - AMB_RS11605 0.57 -5.0 2,500,869 - AMB_RS11605 0.76 +1.4 2,500,889 + AMB_RS11605 0.78 -2.2 2,500,889 + AMB_RS11605 0.78 -3.2 2,500,889 + AMB_RS11605 0.78 -4.5 2,500,889 + AMB_RS11605 0.78 -2.3 2,500,890 - AMB_RS11605 0.79 -0.7 2,500,890 - AMB_RS11605 0.79 -2.9 2,501,054 + +2.8 2,501,054 + -0.2 2,501,055 - -3.6 2,501,079 + +0.2 2,501,376 + +0.4 2,501,377 - +1.9 2,501,377 - -2.4 2,501,391 + +1.9 2,501,391 + +2.0 2,501,392 - -0.7 2,501,392 - +0.0 2,501,440 + -4.7 2,501,440 + -1.3 2,501,441 - -2.4 2,501,885 - -3.0 2,501,885 - -1.6 2,501,885 - -1.3
Or see this region's nucleotide sequence