Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10495 and AMB_RS10500 are separated by 107 nucleotides AMB_RS10500 and AMB_RS10505 are separated by 62 nucleotides AMB_RS10505 and AMB_RS10510 overlap by 4 nucleotides AMB_RS10510 and AMB_RS10515 are separated by 114 nucleotides
AMB_RS10490: AMB_RS10490 - HU family DNA-binding protein, at 2,257,465 to 2,257,743
_RS10490
AMB_RS10495: AMB_RS10495 - transcriptional regulator, at 2,257,733 to 2,258,146
_RS10495
AMB_RS10500: AMB_RS10500 - XRE family transcriptional regulator, at 2,258,254 to 2,258,673
_RS10500
AMB_RS10505: AMB_RS10505 - hypothetical protein, at 2,258,736 to 2,259,350
_RS10505
AMB_RS10510: AMB_RS10510 - transcriptional regulator, at 2,259,347 to 2,259,883
_RS10510
AMB_RS10515: AMB_RS10515 - nucleoside-diphosphate sugar epimerase, at 2,259,998 to 2,260,978
_RS10515
Position (kb)
2258
2259
2260 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2257.778 kb on + strand, within AMB_RS10495 at 2257.778 kb on + strand, within AMB_RS10495 at 2257.918 kb on + strand, within AMB_RS10495 at 2257.930 kb on + strand, within AMB_RS10495 at 2257.930 kb on + strand, within AMB_RS10495 at 2257.930 kb on + strand, within AMB_RS10495 at 2257.931 kb on - strand, within AMB_RS10495 at 2258.042 kb on + strand, within AMB_RS10495 at 2258.043 kb on - strand, within AMB_RS10495 at 2258.063 kb on + strand, within AMB_RS10495 at 2258.063 kb on + strand, within AMB_RS10495 at 2258.063 kb on + strand, within AMB_RS10495 at 2258.063 kb on + strand, within AMB_RS10495 at 2258.064 kb on - strand, within AMB_RS10495 at 2258.064 kb on - strand, within AMB_RS10495 at 2258.109 kb on - strand at 2258.178 kb on + strand at 2258.178 kb on + strand at 2258.178 kb on + strand at 2258.178 kb on + strand at 2258.179 kb on - strand at 2258.179 kb on - strand at 2258.179 kb on - strand at 2258.179 kb on - strand at 2258.179 kb on - strand at 2258.485 kb on - strand, within AMB_RS10500 at 2258.592 kb on - strand, within AMB_RS10500 at 2258.599 kb on + strand, within AMB_RS10500 at 2258.600 kb on - strand, within AMB_RS10500 at 2258.672 kb on - strand at 2258.690 kb on + strand at 2258.690 kb on + strand at 2258.690 kb on + strand at 2258.691 kb on - strand at 2258.691 kb on - strand at 2258.691 kb on - strand at 2258.771 kb on + strand at 2258.822 kb on + strand, within AMB_RS10505 at 2258.907 kb on + strand, within AMB_RS10505 at 2258.908 kb on - strand, within AMB_RS10505 at 2259.278 kb on + strand, within AMB_RS10505 at 2259.278 kb on + strand, within AMB_RS10505 at 2259.279 kb on - strand, within AMB_RS10505 at 2259.802 kb on - strand, within AMB_RS10510 at 2259.921 kb on - strand at 2259.921 kb on - strand at 2259.950 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 2,257,778 + AMB_RS10495 0.11 -1.9 2,257,778 + AMB_RS10495 0.11 +2.1 2,257,918 + AMB_RS10495 0.45 +0.3 2,257,930 + AMB_RS10495 0.48 -2.8 2,257,930 + AMB_RS10495 0.48 +0.1 2,257,930 + AMB_RS10495 0.48 +0.7 2,257,931 - AMB_RS10495 0.48 -3.9 2,258,042 + AMB_RS10495 0.75 +0.1 2,258,043 - AMB_RS10495 0.75 -3.6 2,258,063 + AMB_RS10495 0.80 -0.0 2,258,063 + AMB_RS10495 0.80 +0.4 2,258,063 + AMB_RS10495 0.80 +0.7 2,258,063 + AMB_RS10495 0.80 -0.1 2,258,064 - AMB_RS10495 0.80 -2.1 2,258,064 - AMB_RS10495 0.80 -0.1 2,258,109 - -2.1 2,258,178 + -2.2 2,258,178 + +0.8 2,258,178 + +0.2 2,258,178 + -1.2 2,258,179 - -3.8 2,258,179 - +1.1 2,258,179 - +0.2 2,258,179 - +0.2 2,258,179 - +1.6 2,258,485 - AMB_RS10500 0.55 +0.1 2,258,592 - AMB_RS10500 0.80 -2.4 2,258,599 + AMB_RS10500 0.82 +0.0 2,258,600 - AMB_RS10500 0.82 +0.7 2,258,672 - -3.6 2,258,690 + -2.1 2,258,690 + +0.4 2,258,690 + -2.5 2,258,691 - +0.1 2,258,691 - +0.8 2,258,691 - -3.2 2,258,771 + -0.5 2,258,822 + AMB_RS10505 0.14 -0.1 2,258,907 + AMB_RS10505 0.28 -4.8 2,258,908 - AMB_RS10505 0.28 -1.1 2,259,278 + AMB_RS10505 0.88 -2.7 2,259,278 + AMB_RS10505 0.88 -3.7 2,259,279 - AMB_RS10505 0.88 -0.3 2,259,802 - AMB_RS10510 0.85 -0.9 2,259,921 - +1.4 2,259,921 - +1.2 2,259,950 - -0.7
Or see this region's nucleotide sequence