Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04975 and AMB_RS04980 are separated by 49 nucleotides AMB_RS04980 and AMB_RS04985 overlap by 4 nucleotides AMB_RS04985 and AMB_RS04990 overlap by 4 nucleotides AMB_RS04990 and AMB_RS04995 are separated by 19 nucleotides
AMB_RS04975: AMB_RS04975 - tetratricopeptide repeat protein, at 1,033,406 to 1,034,071
_RS04975
AMB_RS04980: AMB_RS04980 - LemA family protein, at 1,034,121 to 1,034,939
_RS04980
AMB_RS04985: AMB_RS04985 - DNA-binding protein, at 1,034,936 to 1,035,190
_RS04985
AMB_RS04990: AMB_RS04990 - cation transporter, at 1,035,187 to 1,036,077
_RS04990
AMB_RS04995: AMB_RS04995 - hypothetical protein, at 1,036,097 to 1,036,639
_RS04995
Position (kb)
1034
1035
1036 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1033.991 kb on + strand, within AMB_RS04975 at 1033.991 kb on + strand, within AMB_RS04975 at 1033.992 kb on - strand, within AMB_RS04975 at 1033.992 kb on - strand, within AMB_RS04975 at 1033.992 kb on - strand, within AMB_RS04975 at 1034.078 kb on - strand at 1034.078 kb on - strand at 1034.078 kb on - strand at 1034.078 kb on - strand at 1034.078 kb on - strand at 1034.078 kb on - strand at 1034.109 kb on - strand at 1034.109 kb on - strand at 1036.117 kb on + strand at 1036.118 kb on - strand at 1036.118 kb on - strand at 1036.118 kb on - strand at 1036.118 kb on - strand at 1036.130 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 1,033,991 + AMB_RS04975 0.88 +2.3 1,033,991 + AMB_RS04975 0.88 +0.7 1,033,992 - AMB_RS04975 0.88 +2.0 1,033,992 - AMB_RS04975 0.88 +3.7 1,033,992 - AMB_RS04975 0.88 +3.5 1,034,078 - +2.2 1,034,078 - +2.4 1,034,078 - +2.9 1,034,078 - +3.0 1,034,078 - +2.4 1,034,078 - +3.7 1,034,109 - +2.2 1,034,109 - +1.9 1,036,117 + +2.5 1,036,118 - +2.6 1,036,118 - +2.5 1,036,118 - +2.3 1,036,118 - +4.8 1,036,130 - +2.1
Or see this region's nucleotide sequence