Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03405 and AMB_RS03410 are separated by 75 nucleotides AMB_RS03410 and AMB_RS03415 are separated by 97 nucleotides AMB_RS03415 and AMB_RS03420 are separated by 269 nucleotides
AMB_RS03405: AMB_RS03405 - LysR family transcriptional regulator, at 713,495 to 714,388
_RS03405
AMB_RS03410: AMB_RS03410 - thioredoxin-disulfide reductase, at 714,464 to 715,414
_RS03410
AMB_RS03415: AMB_RS03415 - peroxiredoxin, at 715,512 to 716,135
_RS03415
AMB_RS03420: AMB_RS03420 - Lrp/AsnC family transcriptional regulator, at 716,405 to 716,905
_RS03420
Position (kb)
714
715
716 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 713.468 kb on - strand at 713.583 kb on + strand at 713.620 kb on + strand, within AMB_RS03405 at 713.817 kb on + strand, within AMB_RS03405 at 713.818 kb on - strand, within AMB_RS03405 at 713.881 kb on - strand, within AMB_RS03405 at 713.904 kb on + strand, within AMB_RS03405 at 714.420 kb on - strand at 715.443 kb on - strand at 715.458 kb on - strand at 715.458 kb on - strand at 715.458 kb on - strand at 715.498 kb on - strand at 715.503 kb on - strand at 716.136 kb on + strand at 716.220 kb on + strand at 716.221 kb on - strand at 716.255 kb on + strand at 716.258 kb on + strand at 716.258 kb on + strand at 716.367 kb on + strand at 716.369 kb on + strand at 716.390 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 713,468 - -3.7 713,583 + -3.1 713,620 + AMB_RS03405 0.14 +1.0 713,817 + AMB_RS03405 0.36 -1.6 713,818 - AMB_RS03405 0.36 +1.0 713,881 - AMB_RS03405 0.43 -1.8 713,904 + AMB_RS03405 0.46 +4.2 714,420 - +1.7 715,443 - -0.1 715,458 - +2.2 715,458 - +0.6 715,458 - -0.5 715,498 - -2.3 715,503 - -1.0 716,136 + +1.1 716,220 + -3.2 716,221 - +1.0 716,255 + -1.0 716,258 + +0.8 716,258 + -0.6 716,367 + +1.2 716,369 + -0.9 716,390 + -2.9
Or see this region's nucleotide sequence