Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03035 and AMB_RS03040 are separated by 18 nucleotides AMB_RS03040 and AMB_RS03045 overlap by 1 nucleotides AMB_RS03045 and AMB_RS03050 are separated by 118 nucleotides
AMB_RS03035: AMB_RS03035 - aspartate-semialdehyde dehydrogenase, at 635,295 to 636,311
_RS03035
AMB_RS03040: AMB_RS03040 - ABC-type transport system, at 636,330 to 637,214
_RS03040
AMB_RS03045: AMB_RS03045 - hydrogen peroxide-inducible genes activator, at 637,214 to 638,107
_RS03045
AMB_RS03050: AMB_RS03050 - ribose-phosphate pyrophosphokinase, at 638,226 to 639,158
_RS03050
Position (kb) 637
638
639 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 636.298 kb on - strand at 636.309 kb on + strand at 636.309 kb on + strand at 636.487 kb on + strand, within AMB_RS03040 at 636.488 kb on - strand, within AMB_RS03040 at 636.488 kb on - strand, within AMB_RS03040 at 636.491 kb on + strand, within AMB_RS03040 at 636.492 kb on - strand, within AMB_RS03040 at 636.560 kb on + strand, within AMB_RS03040 at 636.560 kb on + strand, within AMB_RS03040 at 636.561 kb on - strand, within AMB_RS03040 at 636.760 kb on + strand, within AMB_RS03040 at 637.068 kb on + strand, within AMB_RS03040 at 637.170 kb on + strand at 637.170 kb on + strand at 637.170 kb on + strand at 637.170 kb on + strand at 637.171 kb on - strand at 637.171 kb on - strand at 637.577 kb on + strand, within AMB_RS03045 at 637.578 kb on - strand, within AMB_RS03045 at 637.676 kb on + strand, within AMB_RS03045 at 638.167 kb on + strand at 638.168 kb on - strand at 638.227 kb on + strand at 638.227 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 636,298 - -2.3 636,309 + +0.7 636,309 + -1.3 636,487 + AMB_RS03040 0.18 +1.8 636,488 - AMB_RS03040 0.18 +1.0 636,488 - AMB_RS03040 0.18 +0.0 636,491 + AMB_RS03040 0.18 +0.5 636,492 - AMB_RS03040 0.18 +1.0 636,560 + AMB_RS03040 0.26 -3.6 636,560 + AMB_RS03040 0.26 -3.7 636,561 - AMB_RS03040 0.26 -2.0 636,760 + AMB_RS03040 0.49 -1.8 637,068 + AMB_RS03040 0.83 +1.1 637,170 + +0.9 637,170 + -1.4 637,170 + -2.6 637,170 + +0.5 637,171 - -0.6 637,171 - +2.2 637,577 + AMB_RS03045 0.41 -0.6 637,578 - AMB_RS03045 0.41 +1.0 637,676 + AMB_RS03045 0.52 +0.0 638,167 + +0.6 638,168 - -0.8 638,227 + -4.3 638,227 + +1.8
Or see this region's nucleotide sequence