Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01515 and AMB_RS01520 are separated by 218 nucleotides AMB_RS01520 and AMB_RS01525 are separated by 15 nucleotides AMB_RS01525 and AMB_RS01530 overlap by 4 nucleotides
AMB_RS01515: AMB_RS01515 - precorrin-3B C(17)-methyltransferase, at 334,516 to 336,309
_RS01515
AMB_RS01520: AMB_RS01520 - Zn-dependent protease, at 336,528 to 337,985
_RS01520
AMB_RS01525: AMB_RS01525 - RNA pyrophosphohydrolase, at 338,001 to 338,507
_RS01525
AMB_RS01530: AMB_RS01530 - divergent polysaccharide deacetylase family protein, at 338,504 to 339,943
_RS01530
Position (kb)
336
337
338 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 336.307 kb on + strand at 336.307 kb on + strand at 336.308 kb on - strand at 336.308 kb on - strand at 336.308 kb on - strand at 336.310 kb on + strand at 336.310 kb on + strand at 336.310 kb on + strand at 336.310 kb on + strand at 336.311 kb on - strand at 336.311 kb on - strand at 336.311 kb on - strand at 336.311 kb on - strand at 336.457 kb on - strand at 336.489 kb on + strand at 336.490 kb on - strand at 336.490 kb on - strand at 336.687 kb on + strand, within AMB_RS01520 at 336.723 kb on + strand, within AMB_RS01520 at 336.723 kb on + strand, within AMB_RS01520 at 336.724 kb on - strand, within AMB_RS01520 at 336.837 kb on + strand, within AMB_RS01520 at 336.837 kb on + strand, within AMB_RS01520 at 336.843 kb on + strand, within AMB_RS01520 at 336.843 kb on + strand, within AMB_RS01520 at 336.843 kb on + strand, within AMB_RS01520 at 336.843 kb on + strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.948 kb on + strand, within AMB_RS01520 at 336.972 kb on - strand, within AMB_RS01520 at 337.059 kb on + strand, within AMB_RS01520 at 337.060 kb on - strand, within AMB_RS01520 at 337.060 kb on - strand, within AMB_RS01520 at 337.072 kb on - strand, within AMB_RS01520 at 337.116 kb on + strand, within AMB_RS01520 at 337.117 kb on - strand, within AMB_RS01520 at 337.335 kb on + strand, within AMB_RS01520 at 337.358 kb on - strand, within AMB_RS01520 at 337.552 kb on - strand, within AMB_RS01520 at 337.825 kb on + strand, within AMB_RS01520 at 337.890 kb on + strand at 337.979 kb on + strand at 337.980 kb on - strand at 338.053 kb on + strand, within AMB_RS01525 at 338.053 kb on + strand, within AMB_RS01525 at 338.054 kb on - strand, within AMB_RS01525 at 338.054 kb on - strand, within AMB_RS01525 at 338.251 kb on + strand, within AMB_RS01525 at 338.492 kb on + strand at 338.492 kb on + strand at 338.492 kb on + strand at 338.493 kb on - strand at 338.493 kb on - strand at 338.781 kb on + strand, within AMB_RS01530 at 338.781 kb on + strand, within AMB_RS01530 at 338.781 kb on + strand, within AMB_RS01530 at 338.781 kb on + strand, within AMB_RS01530 at 338.782 kb on - strand, within AMB_RS01530 at 338.782 kb on - strand, within AMB_RS01530 at 338.782 kb on - strand, within AMB_RS01530 at 338.782 kb on - strand, within AMB_RS01530 at 338.870 kb on - strand, within AMB_RS01530 at 338.871 kb on + strand, within AMB_RS01530 at 338.871 kb on + strand, within AMB_RS01530 at 338.872 kb on - strand, within AMB_RS01530
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 336,307 + +0.0 336,307 + -0.6 336,308 - -0.1 336,308 - -0.4 336,308 - +0.6 336,310 + +1.6 336,310 + -0.0 336,310 + -4.8 336,310 + -3.6 336,311 - -1.0 336,311 - -2.7 336,311 - +2.0 336,311 - -0.4 336,457 - -0.1 336,489 + -1.0 336,490 - -0.6 336,490 - +0.4 336,687 + AMB_RS01520 0.11 +1.2 336,723 + AMB_RS01520 0.13 -1.6 336,723 + AMB_RS01520 0.13 +1.9 336,724 - AMB_RS01520 0.13 +0.2 336,837 + AMB_RS01520 0.21 -0.9 336,837 + AMB_RS01520 0.21 +2.2 336,843 + AMB_RS01520 0.22 -0.0 336,843 + AMB_RS01520 0.22 -0.8 336,843 + AMB_RS01520 0.22 -2.2 336,843 + AMB_RS01520 0.22 -0.6 336,844 - AMB_RS01520 0.22 -0.1 336,844 - AMB_RS01520 0.22 -3.9 336,844 - AMB_RS01520 0.22 +0.6 336,844 - AMB_RS01520 0.22 +2.2 336,844 - AMB_RS01520 0.22 -0.1 336,844 - AMB_RS01520 0.22 +0.3 336,948 + AMB_RS01520 0.29 +1.7 336,972 - AMB_RS01520 0.30 -1.0 337,059 + AMB_RS01520 0.36 +0.1 337,060 - AMB_RS01520 0.36 +0.7 337,060 - AMB_RS01520 0.36 +0.2 337,072 - AMB_RS01520 0.37 +0.7 337,116 + AMB_RS01520 0.40 -1.7 337,117 - AMB_RS01520 0.40 -0.6 337,335 + AMB_RS01520 0.55 +0.5 337,358 - AMB_RS01520 0.57 -1.6 337,552 - AMB_RS01520 0.70 -0.6 337,825 + AMB_RS01520 0.89 -0.3 337,890 + +0.2 337,979 + +1.6 337,980 - -2.2 338,053 + AMB_RS01525 0.10 +0.6 338,053 + AMB_RS01525 0.10 +1.6 338,054 - AMB_RS01525 0.10 -0.6 338,054 - AMB_RS01525 0.10 +0.3 338,251 + AMB_RS01525 0.49 +1.5 338,492 + +1.9 338,492 + +2.2 338,492 + +0.0 338,493 - +1.2 338,493 - +1.4 338,781 + AMB_RS01530 0.19 +0.9 338,781 + AMB_RS01530 0.19 +1.5 338,781 + AMB_RS01530 0.19 +1.0 338,781 + AMB_RS01530 0.19 -0.2 338,782 - AMB_RS01530 0.19 -1.8 338,782 - AMB_RS01530 0.19 +0.4 338,782 - AMB_RS01530 0.19 -2.7 338,782 - AMB_RS01530 0.19 -2.3 338,870 - AMB_RS01530 0.25 -0.6 338,871 + AMB_RS01530 0.25 +1.7 338,871 + AMB_RS01530 0.25 +0.7 338,872 - AMB_RS01530 0.26 -3.4
Or see this region's nucleotide sequence