Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00865 and AMB_RS00870 are separated by 200 nucleotides AMB_RS00870 and AMB_RS00875 are separated by 10 nucleotides AMB_RS00875 and AMB_RS00880 are separated by 4 nucleotides
AMB_RS00865: AMB_RS00865 - formate dehydrogenase subunit alpha, at 193,733 to 196,408
_RS00865
AMB_RS00870: AMB_RS00870 - DUF4863 domain-containing protein, at 196,609 to 197,079
_RS00870
AMB_RS00875: AMB_RS00875 - alpha/beta hydrolase, at 197,090 to 197,878
_RS00875
AMB_RS00880: AMB_RS00880 - 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase, at 197,883 to 199,430
_RS00880
Position (kb) 196
197
198 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 195.733 kb on + strand, within AMB_RS00865 at 195.845 kb on + strand, within AMB_RS00865 at 195.845 kb on + strand, within AMB_RS00865 at 195.845 kb on + strand, within AMB_RS00865 at 195.845 kb on + strand, within AMB_RS00865 at 195.845 kb on + strand, within AMB_RS00865 at 195.845 kb on + strand, within AMB_RS00865 at 195.846 kb on - strand, within AMB_RS00865 at 195.846 kb on - strand, within AMB_RS00865 at 195.846 kb on - strand, within AMB_RS00865 at 195.846 kb on - strand, within AMB_RS00865 at 196.099 kb on + strand, within AMB_RS00865 at 196.406 kb on + strand at 196.593 kb on - strand at 196.593 kb on - strand at 196.646 kb on + strand at 196.647 kb on - strand at 196.979 kb on - strand, within AMB_RS00870 at 196.979 kb on - strand, within AMB_RS00870 at 197.035 kb on + strand at 197.035 kb on + strand at 197.035 kb on + strand at 197.035 kb on + strand at 197.036 kb on - strand at 197.036 kb on - strand at 197.036 kb on - strand at 197.036 kb on - strand at 197.036 kb on - strand at 197.144 kb on + strand at 197.144 kb on + strand at 197.145 kb on - strand at 197.276 kb on + strand, within AMB_RS00875 at 197.276 kb on + strand, within AMB_RS00875 at 197.276 kb on + strand, within AMB_RS00875 at 197.291 kb on + strand, within AMB_RS00875 at 197.333 kb on + strand, within AMB_RS00875 at 197.333 kb on + strand, within AMB_RS00875 at 197.333 kb on + strand, within AMB_RS00875 at 197.333 kb on + strand, within AMB_RS00875 at 197.334 kb on - strand, within AMB_RS00875 at 197.334 kb on - strand, within AMB_RS00875 at 197.334 kb on - strand, within AMB_RS00875 at 197.334 kb on - strand, within AMB_RS00875 at 197.334 kb on - strand, within AMB_RS00875 at 197.334 kb on - strand, within AMB_RS00875 at 197.334 kb on - strand, within AMB_RS00875 at 197.334 kb on - strand, within AMB_RS00875 at 197.549 kb on + strand, within AMB_RS00875 at 197.550 kb on - strand, within AMB_RS00875 at 197.550 kb on - strand, within AMB_RS00875 at 197.550 kb on - strand, within AMB_RS00875 at 197.550 kb on - strand, within AMB_RS00875 at 197.727 kb on - strand, within AMB_RS00875 at 197.745 kb on - strand, within AMB_RS00875 at 197.745 kb on - strand, within AMB_RS00875 at 197.871 kb on + strand at 197.871 kb on + strand at 197.871 kb on + strand at 197.872 kb on - strand at 197.872 kb on - strand at 197.872 kb on - strand at 197.901 kb on + strand at 197.901 kb on + strand at 197.901 kb on + strand at 197.901 kb on + strand at 197.901 kb on + strand at 197.901 kb on + strand at 197.902 kb on - strand at 197.902 kb on - strand at 197.902 kb on - strand at 198.004 kb on + strand at 198.004 kb on + strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.005 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 195,733 + AMB_RS00865 0.75 +2.8 195,845 + AMB_RS00865 0.79 +0.6 195,845 + AMB_RS00865 0.79 +0.0 195,845 + AMB_RS00865 0.79 +0.5 195,845 + AMB_RS00865 0.79 -0.6 195,845 + AMB_RS00865 0.79 -1.3 195,845 + AMB_RS00865 0.79 +2.0 195,846 - AMB_RS00865 0.79 +0.9 195,846 - AMB_RS00865 0.79 -0.0 195,846 - AMB_RS00865 0.79 +1.7 195,846 - AMB_RS00865 0.79 -3.0 196,099 + AMB_RS00865 0.88 -0.4 196,406 + +0.2 196,593 - -0.5 196,593 - -3.1 196,646 + +0.0 196,647 - -3.0 196,979 - AMB_RS00870 0.79 +0.6 196,979 - AMB_RS00870 0.79 +3.2 197,035 + -3.6 197,035 + +0.5 197,035 + -2.6 197,035 + -3.4 197,036 - -0.5 197,036 - -1.3 197,036 - -3.0 197,036 - +0.6 197,036 - -2.1 197,144 + -1.4 197,144 + -1.1 197,145 - +0.6 197,276 + AMB_RS00875 0.24 -1.6 197,276 + AMB_RS00875 0.24 +0.4 197,276 + AMB_RS00875 0.24 -2.8 197,291 + AMB_RS00875 0.25 -1.1 197,333 + AMB_RS00875 0.31 -3.9 197,333 + AMB_RS00875 0.31 -0.7 197,333 + AMB_RS00875 0.31 +1.0 197,333 + AMB_RS00875 0.31 -1.0 197,334 - AMB_RS00875 0.31 +1.9 197,334 - AMB_RS00875 0.31 -1.0 197,334 - AMB_RS00875 0.31 +0.1 197,334 - AMB_RS00875 0.31 -0.3 197,334 - AMB_RS00875 0.31 -0.6 197,334 - AMB_RS00875 0.31 +1.3 197,334 - AMB_RS00875 0.31 -0.1 197,334 - AMB_RS00875 0.31 +0.8 197,549 + AMB_RS00875 0.58 +0.0 197,550 - AMB_RS00875 0.58 -4.7 197,550 - AMB_RS00875 0.58 +0.0 197,550 - AMB_RS00875 0.58 -0.3 197,550 - AMB_RS00875 0.58 -1.8 197,727 - AMB_RS00875 0.81 -0.9 197,745 - AMB_RS00875 0.83 -3.1 197,745 - AMB_RS00875 0.83 -2.8 197,871 + -0.3 197,871 + +0.8 197,871 + +1.7 197,872 - -0.9 197,872 - -0.3 197,872 - -2.4 197,901 + -1.3 197,901 + -0.3 197,901 + -0.2 197,901 + +2.4 197,901 + -1.7 197,901 + +0.6 197,902 - -2.0 197,902 - -4.5 197,902 - -0.5 198,004 + +2.0 198,004 + +0.4 198,005 - -0.8 198,005 - +1.5 198,005 - -0.2 198,005 - -3.4
Or see this region's nucleotide sequence