Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00235 and AMB_RS00240 are separated by 186 nucleotides AMB_RS00240 and AMB_RS00245 overlap by 1 nucleotides AMB_RS00245 and AMB_RS00250 are separated by 69 nucleotides
AMB_RS00235: AMB_RS00235 - radical SAM protein, at 53,514 to 54,716
_RS00235
AMB_RS00240: AMB_RS00240 - hypothetical protein, at 54,903 to 55,520
_RS00240
AMB_RS00245: AMB_RS00245 - transcriptional activator RfaH, at 55,520 to 56,023
_RS00245
AMB_RS00250: AMB_RS00250 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 56,093 to 56,677
_RS00250
Position (kb) 54
55
56 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 54.759 kb on - strand at 55.089 kb on + strand, within AMB_RS00240 at 55.089 kb on + strand, within AMB_RS00240 at 55.089 kb on + strand, within AMB_RS00240 at 55.089 kb on + strand, within AMB_RS00240 at 55.089 kb on + strand, within AMB_RS00240 at 55.089 kb on + strand, within AMB_RS00240 at 55.092 kb on + strand, within AMB_RS00240 at 55.604 kb on + strand, within AMB_RS00245 at 55.604 kb on + strand, within AMB_RS00245 at 55.604 kb on + strand, within AMB_RS00245 at 55.604 kb on + strand, within AMB_RS00245 at 55.616 kb on + strand, within AMB_RS00245 at 55.732 kb on + strand, within AMB_RS00245 at 56.089 kb on + strand at 56.116 kb on - strand at 56.398 kb on + strand, within AMB_RS00250 at 56.482 kb on + strand, within AMB_RS00250 at 56.482 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 54,759 - -3.4 55,089 + AMB_RS00240 0.30 -1.6 55,089 + AMB_RS00240 0.30 -3.6 55,089 + AMB_RS00240 0.30 +0.5 55,089 + AMB_RS00240 0.30 +0.8 55,089 + AMB_RS00240 0.30 -0.6 55,089 + AMB_RS00240 0.30 -3.5 55,092 + AMB_RS00240 0.31 -2.9 55,604 + AMB_RS00245 0.17 -3.4 55,604 + AMB_RS00245 0.17 +1.2 55,604 + AMB_RS00245 0.17 +0.9 55,604 + AMB_RS00245 0.17 +1.0 55,616 + AMB_RS00245 0.19 -1.8 55,732 + AMB_RS00245 0.42 -4.3 56,089 + +1.0 56,116 - -2.3 56,398 + AMB_RS00250 0.52 -2.3 56,482 + AMB_RS00250 0.66 +1.8 56,482 + AMB_RS00250 0.66 +0.4 56,513 + AMB_RS00250 0.72 -1.6 56,513 + AMB_RS00250 0.72 +1.2 56,513 + AMB_RS00250 0.72 +0.1 56,513 + AMB_RS00250 0.72 -1.8 56,513 + AMB_RS00250 0.72 -3.6
Or see this region's nucleotide sequence