Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00010 and AMB_RS00015 are separated by 39 nucleotides AMB_RS00015 and AMB_RS00020 overlap by 20 nucleotides AMB_RS00020 and AMB_RS00025 are separated by 2 nucleotides
AMB_RS00010: AMB_RS00010 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 1,352 to 3,265
_RS00010
AMB_RS00015: AMB_RS00015 - 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG, at 3,305 to 3,937
_RS00015
AMB_RS00020: AMB_RS00020 - ParA family protein, at 3,918 to 4,715
_RS00020
AMB_RS00025: AMB_RS00025 - chromosome partitioning protein ParB, at 4,718 to 5,662
_RS00025
Position (kb) 3
4
5 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 3.009 kb on - strand, within AMB_RS00010 at 3.386 kb on + strand, within AMB_RS00015 at 3.453 kb on + strand, within AMB_RS00015 at 3.453 kb on + strand, within AMB_RS00015 at 3.453 kb on + strand, within AMB_RS00015 at 3.847 kb on + strand, within AMB_RS00015 at 3.847 kb on + strand, within AMB_RS00015 at 5.623 kb on + strand at 5.682 kb on + strand at 5.705 kb on + strand at 5.706 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 3,009 - AMB_RS00010 0.87 -2.3 3,386 + AMB_RS00015 0.13 -2.6 3,453 + AMB_RS00015 0.23 -0.0 3,453 + AMB_RS00015 0.23 -4.9 3,453 + AMB_RS00015 0.23 -0.7 3,847 + AMB_RS00015 0.86 -3.9 3,847 + AMB_RS00015 0.86 -0.3 5,623 + -2.6 5,682 + +1.7 5,705 + +2.4 5,706 - -2.0
Or see this region's nucleotide sequence