Experiment: Cobalt chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_08180 and IAI46_08185 overlap by 7 nucleotides IAI46_08185 and IAI46_08190 overlap by 19 nucleotides IAI46_08190 and IAI46_08195 are separated by 5 nucleotides IAI46_08195 and IAI46_08200 overlap by 1 nucleotides
IAI46_08180: IAI46_08180 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 1,743,010 to 1,743,630
_08180
IAI46_08185: IAI46_08185 - imidazole glycerol phosphate synthase subunit HisF, at 1,743,624 to 1,744,400
_08185
IAI46_08190: IAI46_08190 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,744,382 to 1,745,119
_08190
IAI46_08195: IAI46_08195 - imidazole glycerol phosphate synthase subunit HisH, at 1,745,125 to 1,745,715
_08195
IAI46_08200: IAI46_08200 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 1,745,715 to 1,746,782
_08200
Position (kb)
1744
1745
1746 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1743.409 kb on + strand, within IAI46_08180 at 1743.409 kb on + strand, within IAI46_08180 at 1743.409 kb on + strand, within IAI46_08180 at 1743.409 kb on + strand, within IAI46_08180 at 1743.410 kb on - strand, within IAI46_08180 at 1743.463 kb on - strand, within IAI46_08180 at 1743.463 kb on - strand, within IAI46_08180 at 1743.525 kb on - strand, within IAI46_08180 at 1743.839 kb on - strand, within IAI46_08185 at 1743.967 kb on + strand, within IAI46_08185 at 1743.968 kb on - strand, within IAI46_08185 at 1743.968 kb on - strand, within IAI46_08185 at 1744.252 kb on + strand, within IAI46_08185 at 1744.286 kb on - strand, within IAI46_08185 at 1744.350 kb on + strand at 1744.421 kb on - strand at 1744.426 kb on + strand at 1744.641 kb on + strand, within IAI46_08190 at 1744.765 kb on - strand, within IAI46_08190 at 1744.986 kb on - strand, within IAI46_08190 at 1744.986 kb on - strand, within IAI46_08190 at 1744.986 kb on - strand, within IAI46_08190 at 1745.041 kb on - strand, within IAI46_08190 at 1745.113 kb on + strand at 1745.114 kb on - strand at 1745.114 kb on - strand at 1745.127 kb on - strand at 1745.127 kb on - strand at 1745.282 kb on + strand, within IAI46_08195 at 1745.282 kb on + strand, within IAI46_08195 at 1745.283 kb on - strand, within IAI46_08195 at 1745.283 kb on - strand, within IAI46_08195 at 1745.304 kb on - strand, within IAI46_08195 at 1745.304 kb on - strand, within IAI46_08195 at 1745.478 kb on + strand, within IAI46_08195 at 1745.490 kb on + strand, within IAI46_08195 at 1745.663 kb on + strand at 1745.664 kb on - strand at 1745.744 kb on + strand at 1745.744 kb on + strand at 1745.772 kb on + strand at 1745.863 kb on + strand, within IAI46_08200 at 1745.864 kb on - strand, within IAI46_08200 at 1745.941 kb on + strand, within IAI46_08200 at 1745.942 kb on - strand, within IAI46_08200 at 1746.107 kb on + strand, within IAI46_08200 at 1746.108 kb on - strand, within IAI46_08200 at 1746.109 kb on + strand, within IAI46_08200 at 1746.109 kb on + strand, within IAI46_08200 at 1746.109 kb on + strand, within IAI46_08200 at 1746.109 kb on + strand, within IAI46_08200 at 1746.110 kb on - strand, within IAI46_08200
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 80 uM, aerobic remove 1,743,409 + IAI46_08180 0.64 -2.2 1,743,409 + IAI46_08180 0.64 -2.3 1,743,409 + IAI46_08180 0.64 +0.1 1,743,409 + IAI46_08180 0.64 -0.9 1,743,410 - IAI46_08180 0.64 +0.2 1,743,463 - IAI46_08180 0.73 -2.7 1,743,463 - IAI46_08180 0.73 -4.1 1,743,525 - IAI46_08180 0.83 -0.5 1,743,839 - IAI46_08185 0.28 -3.8 1,743,967 + IAI46_08185 0.44 -0.8 1,743,968 - IAI46_08185 0.44 -4.2 1,743,968 - IAI46_08185 0.44 -3.3 1,744,252 + IAI46_08185 0.81 -0.5 1,744,286 - IAI46_08185 0.85 -3.7 1,744,350 + -1.3 1,744,421 - -1.8 1,744,426 + -2.5 1,744,641 + IAI46_08190 0.35 -1.6 1,744,765 - IAI46_08190 0.52 -2.7 1,744,986 - IAI46_08190 0.82 -4.5 1,744,986 - IAI46_08190 0.82 -1.5 1,744,986 - IAI46_08190 0.82 -3.0 1,745,041 - IAI46_08190 0.89 -3.9 1,745,113 + -3.2 1,745,114 - -1.8 1,745,114 - -0.5 1,745,127 - +0.8 1,745,127 - -2.6 1,745,282 + IAI46_08195 0.27 -0.8 1,745,282 + IAI46_08195 0.27 -2.6 1,745,283 - IAI46_08195 0.27 -2.1 1,745,283 - IAI46_08195 0.27 -2.6 1,745,304 - IAI46_08195 0.30 -3.9 1,745,304 - IAI46_08195 0.30 -2.1 1,745,478 + IAI46_08195 0.60 -2.2 1,745,490 + IAI46_08195 0.62 -3.1 1,745,663 + -1.7 1,745,664 - -1.0 1,745,744 + -1.5 1,745,744 + -1.9 1,745,772 + -1.9 1,745,863 + IAI46_08200 0.14 +0.5 1,745,864 - IAI46_08200 0.14 -0.4 1,745,941 + IAI46_08200 0.21 -2.6 1,745,942 - IAI46_08200 0.21 -2.0 1,746,107 + IAI46_08200 0.37 -1.0 1,746,108 - IAI46_08200 0.37 -2.8 1,746,109 + IAI46_08200 0.37 -0.9 1,746,109 + IAI46_08200 0.37 -4.1 1,746,109 + IAI46_08200 0.37 -1.2 1,746,109 + IAI46_08200 0.37 -2.1 1,746,110 - IAI46_08200 0.37 -1.2
Or see this region's nucleotide sequence