Experiment: 150_nonmag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01040 and AMB_RS01045 are separated by 124 nucleotides AMB_RS01045 and AMB_RS01050 overlap by 4 nucleotides AMB_RS01050 and AMB_RS01055 overlap by 1 nucleotides
AMB_RS01040: AMB_RS01040 - DUF1178 domain-containing protein, at 230,625 to 230,867
_RS01040
AMB_RS01045: AMB_RS01045 - NADH-quinone oxidoreductase subunit NuoB, at 230,992 to 231,528
_RS01045
AMB_RS01050: AMB_RS01050 - hydrogenase expression protein HypE, at 231,525 to 233,042
_RS01050
AMB_RS01055: AMB_RS01055 - hydrogenase 4 subunit F, at 233,042 to 234,532
_RS01055
Position (kb) 231
232
233
234 Strain fitness (log2 ratio) -2
-1
0
1
2 at 230.556 kb on + strand at 230.556 kb on + strand at 230.557 kb on - strand at 230.588 kb on + strand at 230.588 kb on + strand at 230.588 kb on + strand at 230.589 kb on - strand at 230.589 kb on - strand at 230.590 kb on + strand at 230.634 kb on + strand at 230.634 kb on + strand at 230.635 kb on - strand at 230.635 kb on - strand at 230.635 kb on - strand at 230.635 kb on - strand at 230.993 kb on + strand at 230.993 kb on + strand at 230.994 kb on - strand at 231.116 kb on + strand, within AMB_RS01045 at 231.116 kb on + strand, within AMB_RS01045 at 231.116 kb on + strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.117 kb on - strand, within AMB_RS01045 at 231.305 kb on + strand, within AMB_RS01045 at 231.305 kb on + strand, within AMB_RS01045 at 231.306 kb on - strand, within AMB_RS01045 at 231.306 kb on - strand, within AMB_RS01045 at 231.528 kb on + strand at 231.528 kb on + strand at 231.528 kb on + strand at 231.529 kb on - strand at 231.529 kb on - strand at 231.529 kb on - strand at 231.898 kb on + strand, within AMB_RS01050 at 231.907 kb on + strand, within AMB_RS01050 at 232.084 kb on + strand, within AMB_RS01050 at 232.084 kb on + strand, within AMB_RS01050 at 232.224 kb on - strand, within AMB_RS01050 at 232.224 kb on - strand, within AMB_RS01050 at 232.486 kb on + strand, within AMB_RS01050 at 233.097 kb on + strand at 233.097 kb on + strand at 233.098 kb on - strand at 233.098 kb on - strand at 233.136 kb on - strand at 233.423 kb on + strand, within AMB_RS01055 at 233.424 kb on - strand, within AMB_RS01055 at 233.743 kb on - strand, within AMB_RS01055 at 233.982 kb on + strand, within AMB_RS01055 at 233.983 kb on - strand, within AMB_RS01055 at 234.040 kb on - strand, within AMB_RS01055 at 234.040 kb on - strand, within AMB_RS01055
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_1 remove 230,556 + -0.2 230,556 + -0.7 230,557 - +0.1 230,588 + -0.6 230,588 + +0.4 230,588 + -2.3 230,589 - -0.6 230,589 - -0.5 230,590 + -0.6 230,634 + -0.8 230,634 + +1.1 230,635 - -0.2 230,635 - -0.1 230,635 - +0.5 230,635 - -0.6 230,993 + -0.2 230,993 + -0.2 230,994 - -0.9 231,116 + AMB_RS01045 0.23 -0.0 231,116 + AMB_RS01045 0.23 +0.5 231,116 + AMB_RS01045 0.23 +0.2 231,117 - AMB_RS01045 0.23 +0.5 231,117 - AMB_RS01045 0.23 +1.5 231,117 - AMB_RS01045 0.23 -0.1 231,117 - AMB_RS01045 0.23 -1.7 231,117 - AMB_RS01045 0.23 -1.7 231,117 - AMB_RS01045 0.23 -0.0 231,305 + AMB_RS01045 0.58 -0.8 231,305 + AMB_RS01045 0.58 +0.8 231,306 - AMB_RS01045 0.58 +0.0 231,306 - AMB_RS01045 0.58 -0.5 231,528 + -1.3 231,528 + -0.5 231,528 + +0.2 231,529 - +0.9 231,529 - -0.0 231,529 - +0.2 231,898 + AMB_RS01050 0.25 -0.2 231,907 + AMB_RS01050 0.25 -0.6 232,084 + AMB_RS01050 0.37 +0.1 232,084 + AMB_RS01050 0.37 +0.3 232,224 - AMB_RS01050 0.46 +1.3 232,224 - AMB_RS01050 0.46 -0.7 232,486 + AMB_RS01050 0.63 +0.5 233,097 + -0.3 233,097 + -0.2 233,098 - -1.1 233,098 - +0.1 233,136 - -0.3 233,423 + AMB_RS01055 0.26 -0.2 233,424 - AMB_RS01055 0.26 -1.5 233,743 - AMB_RS01055 0.47 +1.9 233,982 + AMB_RS01055 0.63 +0.1 233,983 - AMB_RS01055 0.63 +0.0 234,040 - AMB_RS01055 0.67 -0.4 234,040 - AMB_RS01055 0.67 -0.7
Or see this region's nucleotide sequence